FastQCFastQC Report
Thu 26 May 2016
SRR1545816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545816_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1390191
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.5589160050669296No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44180.3177980579646969No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA32430.23327729786770307No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA24900.17911207884384234No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23190.16681161077866277No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA16610.11947998512434622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.857051E-444.0000041
AACCGTA207.857051E-444.00000421
TCGTTAG408.314601E-944.0000041
GATCGAC207.857051E-444.0000048
TAGCGTA207.857051E-444.00000442
CGCATCG207.857051E-444.00000421
TATATCG408.314601E-944.0000041
TAACGCG408.314601E-944.0000041
TCTAGCG408.314601E-944.0000041
GAGCGTA207.857051E-444.00000420
CGTAAAG207.857051E-444.00000412
CGGTAAT207.857051E-444.0000046
CGATACA408.314601E-944.00000410
CCCGAAC207.857051E-444.00000412
AGCGTTG502.7284841E-1144.01
CTATACG254.4433225E-544.01
TATTGCG302.5281006E-644.01
TAACCGG750.044.02
TATTCGA302.5281006E-644.026
CATCGTA302.5281006E-644.033
Produced by FastQC (version 0.11.5)