FastQCFastQC Report
Thu 26 May 2016
SRR1545815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545815_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2676709
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA192030.7174108205262507No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149270.5576624130602168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30600.11431948710151159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA408.3200575E-944.0000048
CAACGTT551.8189894E-1244.00000431
CCAGGTA207.858358E-444.00000413
CTATACG800.044.0000041
CCGGATG207.858358E-444.0000045
GCACGAC408.3200575E-944.00000420
TGACGTA207.858358E-444.00000427
TCGATCT207.858358E-444.00000418
TAGTTCG2200.044.0000041
GTTACTA207.858358E-444.00000427
ATCCGGT408.3200575E-944.00000437
ATCCGGA408.3200575E-944.00000443
CTGCGAC207.858358E-444.00000423
ACGTGTA408.3200575E-944.0000041
CTCGCTA408.3200575E-944.00000428
GCGATAT408.3200575E-944.0000049
ACCACGT207.858358E-444.00000418
GTTCACG207.858358E-444.00000414
AGTTAGT408.3200575E-944.00000410
CGTCGGT207.858358E-444.00000441
Produced by FastQC (version 0.11.5)