##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545815_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2676709
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.773987011662456	31.0	31.0	33.0	30.0	34.0
2	32.18867870956462	33.0	31.0	34.0	30.0	34.0
3	32.21578849251077	34.0	31.0	34.0	30.0	34.0
4	35.86475033333844	37.0	35.0	37.0	35.0	37.0
5	35.86015999497891	37.0	35.0	37.0	35.0	37.0
6	35.90263939785759	37.0	35.0	37.0	35.0	37.0
7	36.14834858776206	37.0	35.0	37.0	35.0	37.0
8	36.12091527319556	37.0	35.0	37.0	35.0	37.0
9	37.95113402316053	39.0	39.0	39.0	35.0	39.0
10	37.6962138954963	39.0	38.0	39.0	35.0	39.0
11	37.29652270754871	39.0	37.0	39.0	35.0	39.0
12	35.83719335945745	35.0	35.0	39.0	33.0	39.0
13	35.36573306997511	35.0	35.0	39.0	32.0	39.0
14	36.122845628717954	36.0	35.0	41.0	31.0	41.0
15	36.48901617620742	36.0	35.0	41.0	33.0	41.0
16	36.69055022417454	36.0	35.0	41.0	33.0	41.0
17	36.670601473675326	36.0	35.0	41.0	33.0	41.0
18	36.657987476412266	36.0	35.0	40.0	33.0	41.0
19	36.53758365216391	36.0	35.0	40.0	33.0	41.0
20	36.3102937226273	35.0	35.0	40.0	33.0	41.0
21	36.11482533215228	35.0	35.0	40.0	32.0	41.0
22	36.1100026188876	35.0	35.0	40.0	32.0	41.0
23	36.14482784643381	35.0	35.0	40.0	32.0	41.0
24	36.1213927251711	35.0	35.0	40.0	32.0	41.0
25	36.02286016148935	35.0	35.0	40.0	32.0	41.0
26	35.932716630758144	35.0	35.0	40.0	32.0	41.0
27	35.81886189346694	35.0	35.0	40.0	32.0	41.0
28	35.874188415700026	36.0	35.0	40.0	32.0	41.0
29	35.916818376596034	36.0	35.0	40.0	32.0	41.0
30	35.856681469670406	36.0	35.0	40.0	32.0	41.0
31	35.606455165653045	35.0	35.0	40.0	31.0	41.0
32	35.356659988067435	35.0	35.0	40.0	31.0	41.0
33	35.168811028767045	35.0	35.0	40.0	30.0	41.0
34	34.98355069602262	35.0	35.0	40.0	30.0	41.0
35	34.802053192932064	35.0	34.0	40.0	29.0	41.0
36	34.565454817837875	35.0	34.0	40.0	27.0	41.0
37	34.447653443090005	35.0	34.0	40.0	27.0	41.0
38	34.408470625682504	35.0	34.0	40.0	27.0	41.0
39	34.44901257476999	35.0	34.0	40.0	27.0	41.0
40	34.22469943501516	35.0	34.0	40.0	25.0	41.0
41	34.23631294996953	35.0	34.0	40.0	25.0	41.0
42	34.195308866223414	35.0	34.0	40.0	25.0	41.0
43	34.10516234674744	35.0	34.0	39.0	26.0	41.0
44	33.98730082351126	35.0	34.0	39.0	25.0	41.0
45	33.88981843001985	35.0	34.0	39.0	24.0	41.0
46	33.91388567079948	35.0	34.0	39.0	24.0	41.0
47	33.86262010551016	35.0	34.0	39.0	24.0	41.0
48	33.84050974536268	35.0	34.0	39.0	24.0	41.0
49	33.867649789349535	35.0	34.0	39.0	24.0	41.0
50	33.619882848677236	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	3.0
13	8.0
14	13.0
15	40.0
16	135.0
17	393.0
18	910.0
19	1839.0
20	3395.0
21	5810.0
22	8879.0
23	12974.0
24	18744.0
25	28478.0
26	41128.0
27	50553.0
28	53388.0
29	54409.0
30	61351.0
31	72043.0
32	92660.0
33	127266.0
34	286920.0
35	716789.0
36	118887.0
37	139745.0
38	237576.0
39	542365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.384587939891862	22.6682840757064	31.18874707710102	15.75838090730072
2	30.03348514911408	25.591986278672803	31.357274922301976	13.01725364991114
3	27.929707711970188	22.804794992657026	34.52710025632222	14.738397039050566
4	24.533484962317534	26.004433055666492	33.935067278512534	15.527014703503445
5	23.001715913085807	33.042067703287884	30.649764318795953	13.306452064830356
6	20.29585584387395	38.27016683546848	29.517627803395886	11.916349517261683
7	85.00214255640041	5.805263104805192	7.6567531248260465	1.535841213968347
8	88.65819930369719	2.8407271765440325	7.052316856258936	1.4487566634998426
9	85.5136288629059	4.024718413544393	8.235299391902519	2.226353331647183
10	65.7635551716679	16.166718160248276	11.710014050836307	6.359712617247523
11	61.2875362992391	14.1997878738406	16.563175152771556	7.949500674148741
12	51.80936739854799	19.245872450087028	19.692129402187536	9.252630749177442
13	18.559656653001877	52.87350997063932	20.05384223686624	8.512991139492563
14	11.326483379403589	52.488260770969134	27.664904926161192	8.520350923466093
15	9.319205038724792	19.559578572044998	61.894886593948016	9.226329795282192
16	9.91217199927224	13.250076866779317	59.33061830778019	17.507132826168252
17	11.754695785010624	13.464594021987446	29.421689096573438	45.359021096428485
18	20.075921588786827	19.749513301595353	39.78441436853988	20.390150741077942
19	31.824714602894822	20.27175908923981	27.111352037147107	20.79217427071826
20	32.93921752420603	20.231560472206727	25.31156730148851	21.517654702098735
21	19.337290680458725	28.61865073864959	31.06706780602598	20.976990774865705
22	21.379574694148673	23.290802250076492	25.13949032188407	30.190132733890756
23	15.273382351238032	32.68143828858497	25.574464762512473	26.470714597664518
24	17.229814671673314	21.018907920136257	46.2435401083943	15.507737299796132
25	12.196581697898427	22.734297975611096	43.976054176976284	21.093066149514197
26	12.13546186753958	37.2986753509627	30.657385617936058	19.90847716356167
27	15.145351997546241	38.27203480094399	31.05634568419653	15.526267517313238
28	9.899656630586291	28.773094124165162	46.55358501802026	14.773664227228286
29	11.382484984359525	22.387865098522102	44.972688476782494	21.256961440335875
30	14.412735938049298	32.66914707575609	34.932971794842096	17.985145191352515
31	31.646548055840213	25.373845270442175	25.66323048190894	17.316376191808672
32	33.22778083086357	24.97339083180129	28.054450446425065	13.74437789091007
33	30.77712220491656	26.375336280484728	25.335439900265587	17.512101614333123
34	16.703422000673214	28.154125084198544	29.3333716888911	25.809081226237147
35	17.971509043381257	25.051957459701445	34.69689831804653	22.27963517887077
36	35.35677580192692	21.817500520228386	27.0224368805126	15.803286797332097
37	18.785269523134566	33.87932718872317	30.882475457735598	16.452927830406665
38	18.80682584472201	35.852870072914165	22.70743663207319	22.632867450290636
39	19.035987849258174	32.91848310742782	28.836418153785115	19.209110889528898
40	24.556797171451958	24.28470932028846	24.754465278070946	26.404028230188636
41	15.229186288087348	23.3416109110105	26.819127518157558	34.610075282744596
42	22.12074603552347	23.03040786278972	25.198443312291325	29.650402789395486
43	23.276381556605518	25.033016289779724	25.39536423272011	26.29523792089465
44	17.691388940673043	32.47611152351638	29.933362199626483	19.899137336184097
45	14.15338761142881	43.57339553907429	21.149030395160626	21.12418645433628
46	22.097321748460516	35.3882696998441	24.976827888276237	17.537580663419146
47	21.913140352574747	27.453488593642415	26.768132060676002	23.865238993106832
48	24.30376256813871	22.303208903171768	33.06676220687419	20.326266321815332
49	20.984948307791395	21.939478665779507	35.249704020870404	21.825869005558694
50	17.779370114569794	37.69483346900989	25.30446903268155	19.22132738373876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4023.0
1	4402.0
2	4781.0
3	15847.0
4	26913.0
5	20487.0
6	14061.0
7	14901.0
8	15741.0
9	17028.0
10	18315.0
11	17588.5
12	16862.0
13	15434.0
14	14006.0
15	12650.5
16	11295.0
17	11503.0
18	11711.0
19	10254.0
20	8797.0
21	9020.5
22	9244.0
23	9236.5
24	9229.0
25	10492.5
26	11756.0
27	14500.0
28	17244.0
29	17232.0
30	17220.0
31	23711.0
32	30202.0
33	37766.0
34	45330.0
35	46361.0
36	47392.0
37	55069.0
38	62746.0
39	92907.0
40	123068.0
41	199269.5
42	275471.0
43	325039.0
44	374607.0
45	384377.5
46	394148.0
47	339809.0
48	285470.0
49	249891.5
50	214313.0
51	196920.5
52	179528.0
53	160763.5
54	141999.0
55	121211.5
56	100424.0
57	83678.5
58	66933.0
59	56412.0
60	45891.0
61	39636.5
62	33382.0
63	26176.5
64	18971.0
65	15005.0
66	11039.0
67	8296.0
68	5553.0
69	4597.0
70	3641.0
71	2960.5
72	2280.0
73	1918.5
74	1557.0
75	1116.0
76	675.0
77	518.0
78	361.0
79	337.5
80	314.0
81	219.0
82	124.0
83	88.0
84	52.0
85	32.0
86	12.0
87	13.0
88	14.0
89	10.0
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2676709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.45428304742897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.941593223688	9.04219352999123
2	9.81988533552925	2.2495949225289804
3	3.427661607241448	1.1778421872044658
4	1.623821767313757	0.7439885656555245
5	0.9210293961664685	0.527486569934666
6	0.5713123377897207	0.3926383935319087
7	0.3878276903704301	0.31096016973934837
8	0.2959309651043135	0.27117416294429053
9	0.21957059581328484	0.2263521378004188
>10	1.6805331030745032	4.046081306823384
>50	0.44996352949609847	3.7639017210700647
>100	1.1640288612807257	36.14427768543573
>500	0.47373267610261927	36.67711074923482
>1k	0.022448656428556174	3.1358157912664315
>5k	0.0	0.0
>10k+	6.602546008398875E-4	1.2905821068387435
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	19203	0.7174108205262507	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14927	0.5576624130602168	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3060	0.11431948710151159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02772060765664105	0.0
2	0.0	0.0	0.0	0.13680979142671093	0.0
3	0.0	0.0	0.0	0.19479143978669328	0.0
4	0.0	0.0	0.0	0.30982075376890056	0.0
5	0.0	0.0	0.0	0.543391156827283	0.0
6	0.0	0.0	0.0	0.7484190474197979	0.0
7	0.0	0.0	0.0	0.9001725626506281	0.0
8	0.0	0.0	0.0	1.2686847916602066	0.0
9	0.0	0.0	0.0	1.412704929822405	0.0
10	0.0	0.0	0.0	1.6690645116820693	0.0
11	3.7359309510297904E-5	0.0	0.0	1.8578037433280943	0.0
12	3.7359309510297904E-5	0.0	0.0	2.069332153775401	0.0
13	3.7359309510297904E-5	0.0	0.0	2.137923846036308	0.0
14	3.7359309510297904E-5	0.0	0.0	2.1595175269332603	0.0
15	3.7359309510297904E-5	0.0	0.0	2.197885537800336	0.0
16	3.7359309510297904E-5	0.0	0.0	2.2879588330296645	0.0
17	3.7359309510297904E-5	0.0	0.0	2.4057153766061234	0.0
18	3.7359309510297904E-5	0.0	0.0	2.623706947598712	3.7359309510297904E-5
19	3.7359309510297904E-5	0.0	0.0	2.686096994480909	3.7359309510297904E-5
20	3.7359309510297904E-5	0.0	0.0	2.756183059122228	3.7359309510297904E-5
21	3.7359309510297904E-5	0.0	0.0	2.8504032377071993	3.7359309510297904E-5
22	3.7359309510297904E-5	0.0	0.0	2.9349473551290037	3.7359309510297904E-5
23	3.7359309510297904E-5	0.0	0.0	3.0456429892080163	3.7359309510297904E-5
24	3.7359309510297904E-5	0.0	0.0	3.12010009306204	3.7359309510297904E-5
25	3.7359309510297904E-5	0.0	0.0	3.1740842953044206	3.7359309510297904E-5
26	3.7359309510297904E-5	0.0	0.0	3.242377113089245	3.7359309510297904E-5
27	3.7359309510297904E-5	0.0	0.0	3.3018904931391497	3.7359309510297904E-5
28	3.7359309510297904E-5	0.0	0.0	3.367269284782171	3.7359309510297904E-5
29	3.7359309510297904E-5	0.0	0.0	3.4339182929485426	3.7359309510297904E-5
30	3.7359309510297904E-5	0.0	0.0	3.545361113217761	3.7359309510297904E-5
31	3.7359309510297904E-5	0.0	0.0	3.6581488686293504	3.7359309510297904E-5
32	3.7359309510297904E-5	0.0	0.0	3.7399657564569027	3.7359309510297904E-5
33	3.7359309510297904E-5	0.0	0.0	3.8181587912619563	3.7359309510297904E-5
34	3.7359309510297904E-5	0.0	0.0	3.9010964583748176	3.7359309510297904E-5
35	3.7359309510297904E-5	0.0	0.0	4.050496337106499	3.7359309510297904E-5
36	3.7359309510297904E-5	0.0	0.0	4.14972266316585	3.7359309510297904E-5
37	3.7359309510297904E-5	0.0	0.0	4.242336391441879	3.7359309510297904E-5
38	3.7359309510297904E-5	0.0	0.0	4.326955227482704	3.7359309510297904E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	40	8.3200575E-9	44.000004	8
CAACGTT	55	1.8189894E-12	44.000004	31
CCAGGTA	20	7.858358E-4	44.000004	13
CTATACG	80	0.0	44.000004	1
CCGGATG	20	7.858358E-4	44.000004	5
GCACGAC	40	8.3200575E-9	44.000004	20
TGACGTA	20	7.858358E-4	44.000004	27
TCGATCT	20	7.858358E-4	44.000004	18
TAGTTCG	220	0.0	44.000004	1
GTTACTA	20	7.858358E-4	44.000004	27
ATCCGGT	40	8.3200575E-9	44.000004	37
ATCCGGA	40	8.3200575E-9	44.000004	43
CTGCGAC	20	7.858358E-4	44.000004	23
ACGTGTA	40	8.3200575E-9	44.000004	1
CTCGCTA	40	8.3200575E-9	44.000004	28
GCGATAT	40	8.3200575E-9	44.000004	9
ACCACGT	20	7.858358E-4	44.000004	18
GTTCACG	20	7.858358E-4	44.000004	14
AGTTAGT	40	8.3200575E-9	44.000004	10
CGTCGGT	20	7.858358E-4	44.000004	41
>>END_MODULE