FastQCFastQC Report
Thu 26 May 2016
SRR1545814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545814_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3416786
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391271.1451404916784371No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43620.12766383379000032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT219550.042.076071
TAATGCG1700.041.4117621
CGGTCTA3500.038.97142831
CGTTAGG5850.037.9829062
ATAGGGC15500.037.7548374
TAGGGAC32300.037.7337465
AGCGCTA357.2944276E-637.71428340
TAGGGAT38450.037.133945
GGGCGAT48100.037.002087
GTAGGGA40450.036.8751534
AGGGCGA28250.036.8353966
CGAACGT1200.036.66666837
CGTTGAT5000.036.5225
ATAGGGA41900.036.491654
ACGGGCG3800.036.4736825
TAATCGT5100.036.23529421
ATCGTAG1100.036.01
TAAGGGA40400.035.9405944
GTACGGG10550.035.86733
CGGGATC5800.035.275866
Produced by FastQC (version 0.11.5)