##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545814_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3416786
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.668451287262357	31.0	31.0	33.0	30.0	34.0
2	32.08462748325473	33.0	31.0	34.0	30.0	34.0
3	32.06879213389425	33.0	31.0	34.0	30.0	34.0
4	35.82010989274716	37.0	35.0	37.0	35.0	37.0
5	35.831489885524	37.0	35.0	37.0	35.0	37.0
6	35.89930332189373	37.0	35.0	37.0	35.0	37.0
7	36.07366689046373	37.0	35.0	37.0	35.0	37.0
8	36.014158042089846	37.0	35.0	37.0	35.0	37.0
9	37.73927427705452	39.0	38.0	39.0	35.0	39.0
10	37.52022426924016	39.0	37.0	39.0	35.0	39.0
11	37.37124039960361	39.0	37.0	39.0	35.0	39.0
12	36.94720125872677	39.0	35.0	39.0	33.0	39.0
13	36.80554093817992	39.0	35.0	39.0	33.0	39.0
14	37.89777966779307	40.0	36.0	41.0	33.0	41.0
15	38.01304588581199	40.0	36.0	41.0	33.0	41.0
16	38.02853880810797	40.0	36.0	41.0	33.0	41.0
17	37.934681305765125	40.0	36.0	41.0	33.0	41.0
18	37.785383690989136	39.0	36.0	41.0	33.0	41.0
19	37.689712495895265	39.0	36.0	41.0	33.0	41.0
20	37.514073752350896	39.0	35.0	41.0	33.0	41.0
21	37.41944154535871	39.0	35.0	41.0	33.0	41.0
22	37.44017272372341	39.0	35.0	41.0	33.0	41.0
23	37.41250520225733	39.0	35.0	41.0	33.0	41.0
24	37.38101537526787	39.0	35.0	41.0	33.0	41.0
25	37.28058239526854	39.0	35.0	41.0	33.0	41.0
26	37.17935802827569	39.0	35.0	41.0	33.0	41.0
27	37.0755522880274	39.0	35.0	41.0	32.0	41.0
28	36.99097894922304	39.0	35.0	41.0	32.0	41.0
29	36.89340889362108	39.0	35.0	41.0	32.0	41.0
30	36.73367837494066	39.0	35.0	41.0	31.0	41.0
31	36.568548922876644	39.0	35.0	41.0	31.0	41.0
32	36.36034741420739	39.0	35.0	41.0	30.0	41.0
33	36.110178103047716	39.0	35.0	41.0	30.0	41.0
34	35.81974990532038	39.0	35.0	41.0	28.0	41.0
35	35.54187502524302	38.0	35.0	41.0	26.0	41.0
36	35.38310739976106	38.0	35.0	41.0	25.0	41.0
37	35.31438901938839	38.0	35.0	41.0	24.0	41.0
38	35.18069027442749	38.0	35.0	41.0	24.0	41.0
39	35.09500946210854	38.0	35.0	41.0	23.0	41.0
40	34.96890732987082	38.0	34.0	41.0	23.0	41.0
41	34.80473755160551	38.0	34.0	40.0	23.0	41.0
42	34.765085960900095	38.0	34.0	40.0	23.0	41.0
43	34.67842791442016	38.0	34.0	40.0	23.0	41.0
44	34.54093203378848	38.0	34.0	40.0	22.0	41.0
45	34.45052221590699	38.0	34.0	40.0	22.0	41.0
46	34.40420997978802	37.0	34.0	40.0	22.0	41.0
47	34.31618222505009	37.0	34.0	40.0	22.0	41.0
48	34.22290655604419	37.0	34.0	40.0	22.0	41.0
49	34.14324309453387	37.0	34.0	40.0	22.0	41.0
50	34.02108999510066	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	16.0
12	21.0
13	36.0
14	64.0
15	128.0
16	277.0
17	599.0
18	1321.0
19	2712.0
20	4754.0
21	7488.0
22	11702.0
23	17359.0
24	25726.0
25	39007.0
26	57966.0
27	71905.0
28	73531.0
29	72268.0
30	75831.0
31	85374.0
32	100983.0
33	128649.0
34	221770.0
35	357372.0
36	215457.0
37	290105.0
38	499145.0
39	1055212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.520540648433936	21.662140971076326	27.260150328408038	16.557168052081693
2	35.91372125734536	23.272894468661484	27.694857096698478	13.118527177294686
3	29.44433745631128	23.544845945868428	33.96923307459115	13.04158352322914
4	26.708052538262567	25.812327725529194	33.03554861205823	14.444071124150003
5	22.649530874921638	30.354871507902452	32.37437170487119	14.621225912304721
6	20.942136850244644	39.55240392579459	29.75585828319362	9.749600940767142
7	82.94013731032614	4.051087776641557	10.75791694299848	2.250857970033827
8	83.19643079783164	3.4854099729980166	11.063642850327764	2.254516378842573
9	77.62883013451822	5.879033688384347	12.89480816182225	3.5973280152751737
10	38.43096992319683	30.4888278048435	19.206002365966146	11.874199905993526
11	33.36422005943597	23.380978498507076	27.74376270565379	15.511038736403156
12	30.7719301120995	21.953818588580027	31.607188744041913	15.667062555278557
13	23.279508871787698	27.458728758546773	31.788997028201354	17.47276534146417
14	18.612637724458015	29.387207744353905	34.57395341704163	17.42620111414645
15	19.30150146950965	23.363505938036504	40.61489364566584	16.720098946788006
16	22.413783011286046	21.54062326408502	37.77125637953328	18.274337345095653
17	21.611420791351872	21.773766340648784	32.50528420568335	24.10952866231599
18	23.996996007358963	22.898244139375425	34.71742157688541	18.3873382763802
19	25.81987868131045	25.198036985635035	29.62825298394456	19.353831349109953
20	26.730676138335852	24.311794768533936	30.453941218443298	18.503587874686914
21	24.68565488151731	25.057495552838255	31.85127777976145	18.40557178588299
22	23.427747596718085	22.890195639996183	32.01415013992682	21.66790662335891
23	20.813507196529134	25.9527520892441	31.897607868915408	21.336132845311354
24	20.913776865159246	23.709386540450588	36.075218055798636	19.30161853859153
25	20.74630954353009	25.062705126981903	33.16719279463215	21.023792534855858
26	19.781660308839946	27.248619023842874	31.162589638332634	21.80713102898455
27	19.43141888312584	27.01211021117506	32.67201984555076	20.884451060148336
28	18.64491952378639	25.997296874899394	35.111359037411184	20.246424563903037
29	20.751402048591864	24.461467589717355	34.72239701286531	20.064733348825474
30	21.76080093983059	25.722447937915927	33.650307628279904	18.866443493973577
31	25.27518551059387	24.93006585721201	30.535128626726987	19.259620005467127
32	26.453076077928205	24.548713322988327	30.53302138325315	18.465189215830318
33	24.86640954393983	25.91130963425863	29.760745917362108	19.461534904439436
34	20.666848904204127	26.438413175422752	32.06686049404323	20.82787742632989
35	21.48732756455921	26.70164300602964	31.744861984332644	20.066167445078502
36	24.312731321188977	28.161406655260237	28.64788722501204	18.877974798538744
37	22.250764314768322	29.34275076051002	29.185351379922537	19.22113354479912
38	21.88954766262798	29.792032629494503	27.76310837143444	20.555311336443076
39	21.640395389117142	28.573460556206914	28.306425980438927	21.479718074237017
40	23.007411058228406	26.59347117437264	28.17756218855966	22.221555578839293
41	20.154788740061566	26.653732484270304	29.03670291320557	24.15477586246256
42	21.702646873406763	26.67691216248252	27.722368330940245	23.898072633170468
43	21.3767265494532	26.610826665761333	29.32273194750856	22.689714837276902
44	21.245696979559153	27.56394459588631	29.689041104710682	21.501317319843853
45	20.44307135419075	29.321356385796477	28.233609011509646	22.001963248503127
46	21.464616162674513	28.97930979581396	28.92604336355862	20.630030677952906
47	20.98714991222746	27.75757685731562	29.96810452864183	21.287168701815098
48	21.142412782070636	26.469904758448436	31.405566517774307	20.98211594170662
49	21.12104767462756	25.780338598905523	31.842000055022467	21.256613671444452
50	20.38965858558306	27.95794059095302	30.94416799881526	20.70823282464866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2008.0
1	5336.5
2	8665.0
3	42260.5
4	75856.0
5	52258.5
6	28661.0
7	26901.0
8	25141.0
9	24714.5
10	24288.0
11	23688.0
12	23088.0
13	22057.0
14	21026.0
15	19438.5
16	17851.0
17	17296.5
18	16742.0
19	16498.0
20	16254.0
21	16690.5
22	17127.0
23	19343.5
24	21560.0
25	25499.5
26	29439.0
27	32144.5
28	34850.0
29	42037.0
30	49224.0
31	59643.0
32	70062.0
33	79550.5
34	89039.0
35	101684.5
36	114330.0
37	123764.0
38	133198.0
39	146988.0
40	160778.0
41	177697.0
42	194616.0
43	221957.5
44	249299.0
45	256437.0
46	263575.0
47	259065.5
48	254556.0
49	248105.0
50	241654.0
51	240689.0
52	239724.0
53	225186.5
54	210649.0
55	199127.5
56	187606.0
57	178712.0
58	169818.0
59	155746.5
60	141675.0
61	121303.5
62	100932.0
63	85609.0
64	70286.0
65	56928.0
66	43570.0
67	35310.0
68	27050.0
69	21999.5
70	16949.0
71	13685.5
72	10422.0
73	8456.0
74	6490.0
75	5307.5
76	4125.0
77	3058.0
78	1991.0
79	1635.0
80	1279.0
81	953.0
82	627.0
83	476.5
84	326.0
85	253.0
86	180.0
87	148.5
88	117.0
89	76.0
90	35.0
91	27.0
92	19.0
93	16.0
94	13.0
95	11.5
96	10.0
97	6.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3416786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.6265541624449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17319983245258	13.95550695060799
2	6.462203904334202	2.2781277425701947
3	2.369807852535246	1.2531463940189926
4	1.2862585969836982	0.9068922730657418
5	0.8921018015582619	0.7862340361790688
6	0.6726770127900566	0.7114186679865344
7	0.5018106251207932	0.6191634514087397
8	0.4301110213487758	0.6065100170934954
9	0.3799061165832674	0.6026792166539163
>10	5.0834399099159375	21.500823850290402
>50	1.453082447425684	18.340929383664463
>100	1.2898319102471119	36.38796950807065
>500	0.0043876723127420645	0.5144269493029272
>1k	0.001012539764478938	0.37230066482453084
>5k	0.0	0.0
>10k+	1.6875662741315634E-4	1.1638708942624976
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39127	1.1451404916784371	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4362	0.12766383379000032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017121353224931267	0.0
2	0.0	0.0	0.0	0.05756872101442701	0.0
3	0.0	0.0	0.0	0.08979198580186175	0.0
4	0.0	0.0	0.0	0.13217099344237537	0.0
5	0.0	0.0	0.0	0.24110377413159617	0.0
6	0.0	0.0	0.0	0.3828451650176511	0.0
7	0.0	0.0	0.0	0.48112465925580356	0.0
8	0.0	0.0	0.0	0.6932831028926014	0.0
9	0.0	0.0	0.0	0.8057279560382183	0.0
10	0.0	0.0	0.0	0.9790487317613688	0.0
11	0.0	0.0	0.0	1.1064198928466693	0.0
12	0.0	0.0	0.0	1.2220548784735128	0.0
13	0.0	0.0	0.0	1.2725409200342075	0.0
14	0.0	0.0	0.0	1.2973888326632104	0.0
15	0.0	0.0	0.0	1.32516347233921	0.0
16	0.0	0.0	0.0	1.3789567154630111	0.0
17	0.0	0.0	0.0	1.442261821489552	0.0
18	0.0	0.0	0.0	1.5356536815592197	0.0
19	0.0	0.0	0.0	1.5806667435420305	0.0
20	0.0	0.0	0.0	1.6363916265168494	0.0
21	2.9267270469967975E-5	0.0	0.0	1.7033551413521362	0.0
22	2.9267270469967975E-5	0.0	0.0	1.7748550831102679	0.0
23	2.9267270469967975E-5	0.0	0.0	1.8529694279946125	0.0
24	2.9267270469967975E-5	0.0	0.0	1.9135819451379161	0.0
25	2.9267270469967975E-5	0.0	0.0	1.965794755656339	0.0
26	2.9267270469967975E-5	0.0	0.0	2.023334209400296	0.0
27	2.9267270469967975E-5	0.0	0.0	2.084532071952999	0.0
28	2.9267270469967975E-5	0.0	0.0	2.1483347215775295	0.0
29	2.9267270469967975E-5	0.0	0.0	2.2240198830128666	0.0
30	2.9267270469967975E-5	0.0	0.0	2.3129923852415692	0.0
31	2.9267270469967975E-5	0.0	0.0	2.3902872465527545	0.0
32	2.9267270469967975E-5	0.0	0.0	2.4708600421565765	0.0
33	2.9267270469967975E-5	0.0	0.0	2.5528376667429566	0.0
34	2.9267270469967975E-5	0.0	0.0	2.639527321875002	0.0
35	2.9267270469967975E-5	0.0	0.0	2.7421091048722395	0.0
36	2.9267270469967975E-5	0.0	0.0	2.8326035051653804	0.0
37	2.9267270469967975E-5	0.0	0.0	2.9248539416867194	0.0
38	2.9267270469967975E-5	0.0	0.0	3.046196045055207	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21955	0.0	42.07607	1
TAATGCG	170	0.0	41.411762	1
CGGTCTA	350	0.0	38.971428	31
CGTTAGG	585	0.0	37.982906	2
ATAGGGC	1550	0.0	37.754837	4
TAGGGAC	3230	0.0	37.733746	5
AGCGCTA	35	7.2944276E-6	37.714283	40
TAGGGAT	3845	0.0	37.13394	5
GGGCGAT	4810	0.0	37.00208	7
GTAGGGA	4045	0.0	36.875153	4
AGGGCGA	2825	0.0	36.835396	6
CGAACGT	120	0.0	36.666668	37
CGTTGAT	500	0.0	36.52	25
ATAGGGA	4190	0.0	36.49165	4
ACGGGCG	380	0.0	36.473682	5
TAATCGT	510	0.0	36.235294	21
ATCGTAG	110	0.0	36.0	1
TAAGGGA	4040	0.0	35.940594	4
GTACGGG	1055	0.0	35.8673	3
CGGGATC	580	0.0	35.27586	6
>>END_MODULE