FastQCFastQC Report
Thu 26 May 2016
SRR1545813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545813_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2672381
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185560.6943620688816453No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA58000.2170349212930342No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA49770.18623841435783295No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33740.12625445249012024No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA31640.11839629154675176No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA26940.10080897895921277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA551.8189894E-1244.00000431
CGTATTA207.858357E-444.014
GCGATAC2150.044.09
TCGTAAG900.044.01
TCGAATA454.802132E-1044.013
ACGACTC207.858357E-444.021
CGTTTTT110000.041.01
CGGTTAT650.040.61538742
CGGTCTA4650.040.21505731
TCACGAC4700.039.7872325
ATATGCG2050.039.707321
GGTACGC501.3496901E-939.610
AACGATC452.3528628E-839.1111112
AATTCGC900.039.1111113
ACGGTCG452.3528628E-839.1111123
CGACGGT4800.038.95833628
CACGACG4750.038.90526226
TCGTAAT404.1272688E-738.536
ACGCATG1150.038.260871
GTATGCG1150.038.260871
Produced by FastQC (version 0.11.5)