FastQCFastQC Report
Thu 26 May 2016
SRR1545812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545812_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2153027
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115730.5375222883874656No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA100740.4678993807323364No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA22020.10227461151207115No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC207.858014E-444.00000420
GACCGAC207.858014E-444.00000442
ACCGACA207.858014E-444.00000443
CACGAAC207.858014E-444.00000436
TCTACGT207.858014E-444.00000439
AAGTTCG408.3182385E-944.0000041
CGTAGCG207.858014E-444.0000042
AACGCGT650.044.00000439
AACGATC207.858014E-444.00000414
ACCCGTA408.3182385E-944.00000433
CGCACGG650.044.0000042
CACCGCT207.858014E-444.00000424
CGATACA207.858014E-444.00000410
CGCCCGC408.3182385E-944.00000426
CCCGATA207.858014E-444.00000430
GCGAGAC502.7284841E-1144.021
TCCGGCA454.802132E-1044.034
TCACGAG302.5287518E-644.044
TCACGAC2450.044.025
AACGTCG502.7284841E-1144.02
Produced by FastQC (version 0.11.5)