##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545812_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2153027
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72724865967775	31.0	31.0	33.0	30.0	34.0
2	32.13319201291949	33.0	31.0	34.0	30.0	34.0
3	32.16721666751044	33.0	31.0	34.0	30.0	34.0
4	35.86124233462934	37.0	35.0	37.0	35.0	37.0
5	35.83713998941955	37.0	35.0	37.0	35.0	37.0
6	35.88165917101829	37.0	35.0	37.0	35.0	37.0
7	36.12191161559981	37.0	35.0	37.0	35.0	37.0
8	36.087397882144536	37.0	35.0	37.0	35.0	37.0
9	37.91513901126182	39.0	39.0	39.0	35.0	39.0
10	37.625842592777516	39.0	37.0	39.0	35.0	39.0
11	37.26824977113617	39.0	37.0	39.0	34.0	39.0
12	36.113656261626076	37.0	35.0	39.0	33.0	39.0
13	35.738984230109516	37.0	35.0	39.0	32.0	39.0
14	36.58873994613165	38.0	35.0	41.0	32.0	41.0
15	36.876422357917484	38.0	35.0	41.0	33.0	41.0
16	37.04815685079657	38.0	35.0	41.0	33.0	41.0
17	37.02103132009027	38.0	35.0	41.0	33.0	41.0
18	36.97459948249604	38.0	35.0	41.0	33.0	41.0
19	36.890190880095794	37.0	35.0	41.0	33.0	41.0
20	36.69844409754267	36.0	35.0	41.0	33.0	41.0
21	36.51749467145558	36.0	35.0	41.0	32.0	41.0
22	36.498384832145625	36.0	35.0	41.0	32.0	41.0
23	36.51260620512423	36.0	35.0	40.0	32.0	41.0
24	36.49191812271746	36.0	35.0	41.0	33.0	41.0
25	36.41059726608166	36.0	35.0	40.0	32.0	41.0
26	36.287412094692726	35.0	35.0	40.0	32.0	41.0
27	36.19706673441624	36.0	35.0	40.0	32.0	41.0
28	36.24803079571227	36.0	35.0	40.0	32.0	41.0
29	36.257856496922706	36.0	35.0	40.0	32.0	41.0
30	36.18795537631437	36.0	35.0	40.0	32.0	41.0
31	35.965765408422655	36.0	35.0	40.0	31.0	41.0
32	35.753679819156936	35.0	35.0	40.0	31.0	41.0
33	35.58379388646775	36.0	35.0	40.0	30.0	41.0
34	35.41670633949319	36.0	35.0	40.0	30.0	41.0
35	35.20149956317315	35.0	35.0	40.0	29.0	41.0
36	35.01751719788001	35.0	35.0	40.0	28.0	41.0
37	34.9204320243081	35.0	34.0	40.0	27.0	41.0
38	34.87700293586657	35.0	34.0	40.0	27.0	41.0
39	34.873172514789644	35.0	34.0	40.0	27.0	41.0
40	34.69353798164166	35.0	34.0	40.0	26.0	41.0
41	34.63191311581323	35.0	34.0	40.0	26.0	41.0
42	34.623118056578015	35.0	34.0	40.0	26.0	41.0
43	34.53276665829086	35.0	34.0	40.0	26.0	41.0
44	34.408661851430566	35.0	34.0	40.0	26.0	41.0
45	34.29581421877199	35.0	34.0	40.0	24.0	41.0
46	34.26657399094391	35.0	34.0	40.0	25.0	41.0
47	34.217056265434664	35.0	34.0	40.0	24.0	41.0
48	34.18429169722442	35.0	34.0	40.0	24.0	41.0
49	34.17406562946029	35.0	34.0	40.0	24.0	41.0
50	33.96357268162452	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	7.0
13	11.0
14	27.0
15	55.0
16	112.0
17	309.0
18	629.0
19	1423.0
20	2559.0
21	4387.0
22	6745.0
23	10042.0
24	14877.0
25	22410.0
26	31974.0
27	38873.0
28	40753.0
29	42191.0
30	47201.0
31	56135.0
32	70822.0
33	96382.0
34	204509.0
35	480164.0
36	107965.0
37	131502.0
38	225449.0
39	515510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.75857664581076	23.380756488423042	29.492709566577663	14.36795729918854
2	29.850206244510634	25.288860752791305	31.088509340570276	13.772423662127785
3	27.579496216257386	24.76295002338568	33.707798369458445	13.949755390898488
4	25.286724225938645	27.06115622330793	32.60502538983487	15.047094160918558
5	22.511515183042295	32.073726897061675	30.88786160136403	14.526896318532001
6	19.976015163767105	39.99401772481255	29.20539315113094	10.824573960289397
7	86.54587239268248	4.4435578374075195	7.3661407869014175	1.6444289830085734
8	88.5774307521457	3.1923891339959973	6.785841515224844	1.444338598633459
9	85.70598510840783	4.123914841755352	7.996044638548424	2.174055411288386
10	58.07400464555251	22.300231255808683	11.728139034020474	7.8976250646183255
11	51.55708683634715	17.176468293244813	20.996206735911812	10.270238134496223
12	47.588906223656274	19.553958217895083	22.717132669492766	10.140002888955875
13	20.956216526778345	45.96184813288453	22.026570033724614	11.055365306612504
14	12.652558467682942	47.13939955235118	27.063153411452806	13.144888568513075
15	11.238038352514854	22.766923034406908	54.115020387575264	11.880018225502978
16	12.60169983934247	16.61261098908653	52.56636354304892	18.219325628522075
17	13.415252107846301	16.46858121147575	30.827899510781798	39.28826716989615
18	19.46910094485578	21.853697143602936	37.388941244118165	21.28826066742312
19	29.24361840329917	23.506718680258075	26.511790144758983	20.737872771683776
20	32.054776832803306	20.43936281337856	27.080292072510005	20.42556828130813
21	20.600809929462102	28.851519279600303	28.959042315772166	21.588628475165432
22	21.96279935179633	23.508437190987387	26.524330628459374	28.004432828756908
23	17.26466969527089	31.401185400833338	26.36627408759853	24.967870816297243
24	17.861410934465756	21.80845850980968	43.68923380895827	16.6408967467663
25	15.111840213801313	24.21195832657928	40.11333810490997	20.56286335470944
26	14.455554900147561	36.43892993445972	28.915289961528583	20.19022520386414
27	14.965859694281585	37.40217842135747	29.554065044237714	18.07789684012323
28	12.124557657660587	28.538007187090546	42.272112704578255	17.065322450670614
29	12.726082859155971	22.73854438425528	42.40411290708384	22.131259849504907
30	15.707002280974647	32.262577292342364	33.364653578427024	18.665766848255966
31	29.238184193695666	26.960228552637748	25.43219383686317	18.369393416803412
32	30.75261016234353	26.4906106611761	26.347463362047947	16.409315814432425
33	28.046652457214886	28.27488926056199	25.924663276401084	17.753795005822035
34	18.000006502473028	27.69839858023146	28.949474391171126	25.352120526124384
35	19.635564254419474	26.001717581804595	32.54320544981554	21.819512713960393
36	30.49618049378851	24.65022500878995	27.02604286894684	17.8275516284747
37	19.249503141391166	32.97496965899638	30.273377900044913	17.50214929956754
38	18.90264265148556	34.02168203185562	25.933302276283577	21.142373040375247
39	19.270636178738123	33.357872427981626	27.36900187503454	20.002489518245707
40	24.757608706254032	26.644301255859776	24.530718843748826	24.067371194137372
41	15.752751823363106	24.62161412745869	28.303918158016593	31.321715891161606
42	21.51250309448047	25.01877589087364	24.33007110454258	29.13864991010331
43	21.92907938451306	26.477373483936802	25.11919265294862	26.474354478601526
44	18.552438032593184	31.967179231844284	28.502940278965383	20.977442456597153
45	15.946897089539519	40.75824409076152	21.737163537661164	21.557695282037802
46	21.15737517457979	35.38269608323537	25.5914115336222	17.868517208562643
47	20.739033927581957	27.975311038830448	27.13133648579419	24.15431854779341
48	22.912021075444013	23.58103265774187	31.715301294410146	21.79164497240397
49	20.336437954563504	23.846426449830865	33.9919564408621	21.82517915474353
50	18.52893623721393	34.54522400322894	26.95883516555993	19.967004593997196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1470.0
1	2457.5
2	3445.0
3	11981.0
4	20517.0
5	15547.5
6	10578.0
7	10746.0
8	10914.0
9	11632.5
10	12351.0
11	12207.0
12	12063.0
13	11325.0
14	10587.0
15	9873.5
16	9160.0
17	8975.5
18	8791.0
19	7918.0
20	7045.0
21	7584.0
22	8123.0
23	7461.0
24	6799.0
25	8731.5
26	10664.0
27	14521.5
28	18379.0
29	18859.5
30	19340.0
31	25356.5
32	31373.0
33	38630.5
34	45888.0
35	52671.5
36	59455.0
37	63503.5
38	67552.0
39	89590.0
40	111628.0
41	149859.5
42	188091.0
43	233796.5
44	279502.0
45	277273.0
46	275044.0
47	240061.5
48	205079.0
49	190806.0
50	176533.0
51	165406.0
52	154279.0
53	137024.0
54	119769.0
55	103917.5
56	88066.0
57	77800.0
58	67534.0
59	55375.0
60	43216.0
61	35851.0
62	28486.0
63	23374.0
64	18262.0
65	14372.5
66	10483.0
67	7980.0
68	5477.0
69	4167.0
70	2857.0
71	2329.5
72	1802.0
73	1467.0
74	1132.0
75	860.0
76	588.0
77	435.5
78	283.0
79	264.0
80	245.0
81	176.0
82	107.0
83	73.0
84	39.0
85	27.5
86	16.0
87	11.5
88	7.0
89	6.5
90	6.0
91	3.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2153027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.221855181621734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3398203045242	11.283594345068346
2	8.96086327505386	2.548801996002573
3	3.074400965828007	1.3117105591873173
4	1.4260877227282793	0.8112645227572126
5	0.8374521544084336	0.5955061630766932
6	0.5653696589053708	0.48243632478210363
7	0.38793234186347086	0.386198231037476
8	0.2930745898271251	0.3334451499147651
9	0.2466980384838768	0.3157653399217057
>10	2.432564749183499	7.928036007233572
>50	0.7342566890800989	7.5018637982677845
>100	1.591440147739107	56.13675810032899
>500	0.1057435314112355	8.703407958280884
>1k	0.0036349338922612206	0.6438909929339691
>5k	0.0	0.0
>10k+	6.60897071320222E-4	1.0173205112066002
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11573	0.5375222883874656	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	10074	0.4678993807323364	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	2202	0.10227461151207115	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04379880047951094	0.0
2	0.0	0.0	0.0	0.15540910541298367	0.0
3	0.0	0.0	0.0	0.21904044863348207	0.0
4	0.0	0.0	0.0	0.3298611675561895	0.0
5	0.0	0.0	0.0	0.5714744868503739	0.0
6	0.0	0.0	0.0	0.7713790862817791	0.0
7	0.0	0.0	0.0	0.924698111078031	0.0
8	0.0	0.0	0.0	1.2951532888347428	0.0
9	0.0	0.0	0.0	1.4331915020108899	0.0
10	0.0	0.0	0.0	1.696170089831665	0.0
11	0.0	0.0	0.0	1.9280296995811015	0.0
12	0.0	0.0	0.0	2.1507858470887733	0.0
13	0.0	0.0	0.0	2.2317880825461085	0.0
14	0.0	0.0	0.0	2.2576586359576543	0.0
15	0.0	0.0	0.0	2.3007607428982544	0.0
16	0.0	0.0	0.0	2.411070553225761	0.0
17	0.0	0.0	0.0	2.5524528953886785	0.0
18	0.0	0.0	0.0	2.7721435913251438	0.0
19	0.0	0.0	0.0	2.861924165372752	0.0
20	0.0	0.0	0.0	2.954909529699349	0.0
21	0.0	0.0	0.0	3.0770166839524076	0.0
22	0.0	0.0	0.0	3.2057192037071527	0.0
23	0.0	0.0	0.0	3.362568142433885	0.0
24	0.0	0.0	0.0	3.471995474278771	0.0
25	0.0	0.0	0.0	3.5605219999563404	0.0
26	0.0	0.0	0.0	3.662843057704339	0.0
27	0.0	0.0	0.0	3.754992389784243	0.0
28	0.0	0.0	0.0	3.8476061842234213	0.0
29	0.0	0.0	0.0	3.95698706983238	0.0
30	0.0	0.0	0.0	4.1033391592395265	0.0
31	0.0	0.0	0.0	4.245046625053936	0.0
32	0.0	0.0	0.0	4.3588863493119225	0.0
33	0.0	0.0	0.0	4.473701444524384	0.0
34	0.0	0.0	0.0	4.60467982983957	0.0
35	0.0	0.0	0.0	4.782568913441401	0.0
36	0.0	0.0	0.0	4.924694395379157	0.0
37	0.0	0.0	0.0	5.064636904228326	0.0
38	0.0	0.0	0.0	5.19584752072315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCCGC	20	7.858014E-4	44.000004	20
GACCGAC	20	7.858014E-4	44.000004	42
ACCGACA	20	7.858014E-4	44.000004	43
CACGAAC	20	7.858014E-4	44.000004	36
TCTACGT	20	7.858014E-4	44.000004	39
AAGTTCG	40	8.3182385E-9	44.000004	1
CGTAGCG	20	7.858014E-4	44.000004	2
AACGCGT	65	0.0	44.000004	39
AACGATC	20	7.858014E-4	44.000004	14
ACCCGTA	40	8.3182385E-9	44.000004	33
CGCACGG	65	0.0	44.000004	2
CACCGCT	20	7.858014E-4	44.000004	24
CGATACA	20	7.858014E-4	44.000004	10
CGCCCGC	40	8.3182385E-9	44.000004	26
CCCGATA	20	7.858014E-4	44.000004	30
GCGAGAC	50	2.7284841E-11	44.0	21
TCCGGCA	45	4.802132E-10	44.0	34
TCACGAG	30	2.5287518E-6	44.0	44
TCACGAC	245	0.0	44.0	25
AACGTCG	50	2.7284841E-11	44.0	2
>>END_MODULE