FastQCFastQC Report
Thu 26 May 2016
SRR1545811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545811_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2808279
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219670.7822228489405789No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA86280.3072344307670285No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC32270.11491023505855365No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA29700.10575872269101468No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGA207.858425E-444.00000414
ATACGCC207.858425E-444.00000413
CGAACTA254.4444874E-544.030
CGCTAAT351.4470788E-744.024
AACGCGT254.4444874E-544.014
TATTACG254.4444874E-544.01
TATAGCG1500.044.01
CGCAACT302.5290283E-644.013
ATCGAGT302.5290283E-644.030
TAATCCG551.8189894E-1244.01
CGTTTTT122300.041.6255071
TTACGAG1650.041.3333321
TCACGAC4400.041.025
ACCCACG650.040.61538325
CGGTCTA4400.040.031
GTGCGTA557.8216544E-1140.016
CGGGTAT2700.039.9259266
CATGACG1050.039.8095251
CGAATAT4650.039.74193614
CGTAAAC501.3496901E-939.643
Produced by FastQC (version 0.11.5)