##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545811_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2808279
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.636047914042727	31.0	31.0	33.0	30.0	34.0
2	32.04594344080485	33.0	31.0	34.0	30.0	34.0
3	32.085781006801675	33.0	31.0	34.0	30.0	34.0
4	35.80168743917538	37.0	35.0	37.0	35.0	37.0
5	35.78704039021764	37.0	35.0	37.0	35.0	37.0
6	35.84206519366487	37.0	35.0	37.0	35.0	37.0
7	36.040325053173135	37.0	35.0	37.0	35.0	37.0
8	35.974013265775945	37.0	35.0	37.0	35.0	37.0
9	37.70391154155267	39.0	38.0	39.0	35.0	39.0
10	37.47931882836428	39.0	37.0	39.0	35.0	39.0
11	37.23701704851975	39.0	37.0	39.0	34.0	39.0
12	36.55351195518679	39.0	35.0	39.0	33.0	39.0
13	36.31187784404612	39.0	35.0	39.0	33.0	39.0
14	37.29123922516246	39.0	35.0	41.0	33.0	41.0
15	37.465799516358594	39.0	35.0	41.0	33.0	41.0
16	37.549513064763154	39.0	35.0	41.0	33.0	41.0
17	37.47441511331317	39.0	35.0	41.0	33.0	41.0
18	37.39473820086964	39.0	35.0	41.0	33.0	41.0
19	37.313786486314214	39.0	35.0	41.0	33.0	41.0
20	37.14320692495297	39.0	35.0	41.0	33.0	41.0
21	37.02971962543608	38.0	35.0	41.0	32.0	41.0
22	37.059630827278916	39.0	35.0	41.0	32.0	41.0
23	37.07870977207037	39.0	35.0	41.0	33.0	41.0
24	37.035046375377945	38.0	35.0	41.0	33.0	41.0
25	36.93278588060517	38.0	35.0	41.0	32.0	41.0
26	36.829258773789924	38.0	35.0	41.0	32.0	41.0
27	36.72589653663329	38.0	35.0	41.0	32.0	41.0
28	36.688376404196305	38.0	35.0	41.0	32.0	41.0
29	36.63194006008662	38.0	35.0	41.0	32.0	41.0
30	36.501951195020155	38.0	35.0	41.0	31.0	41.0
31	36.34416309775489	38.0	35.0	41.0	31.0	41.0
32	36.15039602546613	38.0	35.0	41.0	30.0	41.0
33	35.96029240684419	38.0	35.0	41.0	30.0	41.0
34	35.74081706269213	38.0	35.0	41.0	29.0	41.0
35	35.52490546701378	38.0	35.0	41.0	28.0	41.0
36	35.36425796724613	38.0	35.0	40.0	27.0	41.0
37	35.28876689246332	38.0	34.0	40.0	27.0	41.0
38	35.195879398022775	38.0	34.0	40.0	27.0	41.0
39	35.13131138323507	38.0	34.0	40.0	26.0	41.0
40	34.97581686150129	37.0	34.0	40.0	25.0	41.0
41	34.87567723862195	37.0	34.0	40.0	24.0	41.0
42	34.87143691919499	37.0	34.0	40.0	24.0	41.0
43	34.79177674298031	37.0	34.0	40.0	24.0	41.0
44	34.64884115858859	37.0	34.0	40.0	24.0	41.0
45	34.556693975206876	37.0	34.0	40.0	23.0	41.0
46	34.503303624746685	36.0	34.0	40.0	23.0	41.0
47	34.42714488126002	36.0	34.0	40.0	23.0	41.0
48	34.3611720915194	36.0	34.0	40.0	23.0	41.0
49	34.315050249636876	36.0	34.0	40.0	24.0	41.0
50	34.13914571878364	36.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	6.0
13	30.0
14	50.0
15	119.0
16	230.0
17	516.0
18	1142.0
19	2223.0
20	3942.0
21	6292.0
22	9347.0
23	13739.0
24	20082.0
25	29189.0
26	41626.0
27	51455.0
28	55408.0
29	57269.0
30	63007.0
31	73518.0
32	90363.0
33	118512.0
34	219890.0
35	409334.0
36	169123.0
37	219938.0
38	368156.0
39	783764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.504299608407855	22.269154880978707	28.071284939993497	15.15526057061994
2	33.116189666340134	24.72304211939056	28.597977622593767	13.562790591675542
3	29.456795425240866	24.30609636720568	32.653664397305256	13.583443810248196
4	25.928691558068127	26.62516794093464	32.237573261061314	15.208567239935919
5	22.797414359470693	31.02672490874304	31.730501136105065	14.445359595681198
6	20.48136955053255	39.70620440490421	28.84816643930322	10.964259605260018
7	83.54066672150452	4.738097603550075	9.400419260337024	2.3208164146083776
8	83.95978462253927	4.0883758344523455	9.372893505239329	2.578946037769039
9	79.1424569994648	5.631954659775613	11.239445938241891	3.9861424025176984
10	47.09795572306028	24.5521545402006	17.33367660406961	11.01621313266951
11	39.54211814424421	21.715221315261054	24.678210391488882	14.06445014900585
12	36.45431953164198	20.78137535479915	28.08983722771135	14.674467885847525
13	23.313281906819086	33.64017606512743	28.053551659219046	14.992990368834436
14	16.952624721404106	36.07946361454827	30.91776137627351	16.050150287774116
15	16.175778831091925	23.643377314006194	44.78885466864225	15.391989186259627
16	18.570946832561862	19.93729967713322	42.47042405686899	19.021329433435923
17	19.04151973504057	19.26186821181229	31.588599280911904	30.108012772235238
18	21.080633370117425	22.684961145242337	35.5680828008898	20.66632268375044
19	26.720137137371324	23.808816716572675	29.318276424813916	20.152769721242084
20	27.783955938850806	23.560586394727874	28.781542004907635	19.87391566151369
21	22.267623694084527	26.726155057955424	31.137504500087065	19.868716747872988
22	21.84437514933523	23.532882594642484	29.956282833721293	24.66645942230099
23	19.140370312209008	27.401942613251745	29.468368349441064	23.989318725098183
24	19.162982025646315	23.384784773877524	38.302390894921764	19.149842305554397
25	18.807924711184324	24.271092722624783	35.47952322401015	21.441459342180742
26	17.656116076785818	28.982554796015634	30.854733450629375	22.506595676569173
27	17.974816604760424	30.26928592208965	30.915553618426088	20.840343854723837
28	16.40713048810321	26.254015359584997	37.2486138307483	20.090240321563492
29	17.62944493762906	23.096886028774207	37.28664424012001	21.987024793476717
30	19.226686522243693	28.126407668183965	33.58405628500587	19.06284952456647
31	26.5105425778564	25.779561076374534	28.443220919289004	19.266675426480063
32	28.234374148722402	24.33643523310896	28.69800329668099	18.731187321487646
33	26.426718997649452	25.73989265311602	28.074169268794165	19.759219080440367
34	19.351602885610724	26.192732274820273	31.588100754946357	22.867564084622646
35	20.517548292032238	26.357103407460585	32.0209637290312	21.104384571475983
36	26.40364436724414	26.048622661779685	28.722253024005095	18.82547994697108
37	20.892154946143172	30.773188846264922	29.59955189637497	18.73510431121694
38	20.670453327464973	31.40731387444054	27.061769859761085	20.860462938333406
39	20.39601478343142	29.98252666490758	28.79713874582974	20.82431980583126
40	23.2741832275212	26.53087531545121	28.11365964706498	22.081281809962615
41	17.954946784133625	25.56245301837887	29.14037387310876	27.34222632437874
42	21.02672134784329	25.487994604524694	27.215244639154445	26.270039408477576
43	21.414930639014145	25.7557386570209	28.599081501517475	24.230249202447478
44	19.936195798209507	27.72463134895073	29.398147406294033	22.941025446545734
45	18.476689816075968	32.62610303321002	26.47938470500972	22.417822445704292
46	21.4569136471127	31.432738698683426	27.532164717252094	19.578182936951777
47	21.057238258734266	27.033211443734757	29.454089141427897	22.45546115610308
48	21.300091621950667	25.88450079212215	31.27175042080933	21.543657165117853
49	20.846041294330085	24.797144443269346	33.22547367978751	21.131340582613053
50	19.80016942761029	30.062326428392623	29.37083530518157	20.766668838815516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2131.0
1	4534.0
2	6937.0
3	24205.0
4	41473.0
5	29005.5
6	16538.0
7	16445.5
8	16353.0
9	16289.0
10	16225.0
11	15436.0
12	14647.0
13	14156.5
14	13666.0
15	12729.0
16	11792.0
17	11429.0
18	11066.0
19	10818.5
20	10571.0
21	11466.0
22	12361.0
23	13310.0
24	14259.0
25	15597.0
26	16935.0
27	24653.5
28	32372.0
29	35714.5
30	39057.0
31	45006.5
32	50956.0
33	61575.0
34	72194.0
35	80470.5
36	88747.0
37	93313.0
38	97879.0
39	119597.5
40	141316.0
41	173099.0
42	204882.0
43	226569.0
44	248256.0
45	260650.0
46	273044.0
47	257018.5
48	240993.0
49	232497.5
50	224002.0
51	208255.5
52	192509.0
53	176393.0
54	160277.0
55	149030.5
56	137784.0
57	130496.0
58	123208.0
59	107809.5
60	92411.0
61	80325.0
62	68239.0
63	55973.0
64	43707.0
65	36017.0
66	28327.0
67	22518.0
68	16709.0
69	13611.0
70	10513.0
71	8857.0
72	7201.0
73	5486.5
74	3772.0
75	3002.0
76	2232.0
77	1738.0
78	1244.0
79	968.0
80	692.0
81	560.5
82	429.0
83	289.0
84	149.0
85	107.0
86	65.0
87	76.0
88	87.0
89	60.5
90	34.0
91	27.5
92	21.0
93	15.5
94	10.0
95	8.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2808279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.687520265590848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77805859275813	13.312979695161452
2	7.8459303658318795	2.618582439644682
3	2.6610471668815383	1.3321883557508634
4	1.3509403468748453	0.9017537766431324
5	0.8347349136144214	0.696482789366844
6	0.5856625310038398	0.5863953212954282
7	0.4286716594678318	0.500742690325773
8	0.3456642555718861	0.4614623415956979
9	0.28256347175207247	0.4243755295060574
>10	3.371193435876002	13.294133261420685
>50	0.9667963677637167	11.51719087849456
>100	1.53381816533381	51.394629637446045
>500	0.012756592883089286	1.3257117417980573
>1k	0.0017297075095714285	0.5294837060361562
>5k	2.1621343869642856E-4	0.31130394655400045
>10k+	2.1621343869642856E-4	0.7925838889605618
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21967	0.7822228489405789	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	8628	0.3072344307670285	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC	3227	0.11491023505855365	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	2970	0.10575872269101468	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04878432662851519	0.0
2	0.0	0.0	0.0	0.18513117820558428	0.0
3	0.0	0.0	0.0	0.2563135642861696	0.0
4	0.0	0.0	0.0	0.38350890349569966	0.0
5	0.0	0.0	0.0	0.7219368161069466	0.0
6	0.0	0.0	0.0	0.9694905669985069	0.0
7	0.0	0.0	0.0	1.1671917213353802	0.0
8	0.0	0.0	0.0	1.6031170692085794	0.0
9	0.0	0.0	0.0	1.7631082951515857	0.0
10	0.0	0.0	0.0	2.1306643677497856	0.0
11	0.0	0.0	0.0	2.358918041975174	0.0
12	0.0	0.0	0.0	2.620751000879898	0.0
13	0.0	0.0	0.0	2.716824076240288	0.0
14	0.0	0.0	0.0	2.7530384267375143	0.0
15	0.0	0.0	0.0	2.809692341822162	0.0
16	0.0	0.0	0.0	2.9221455560505207	0.0
17	0.0	0.0	0.0	3.050587210173918	0.0
18	0.0	0.0	0.0	3.2529175341908694	0.0
19	0.0	0.0	0.0	3.339767879188642	0.0
20	0.0	0.0	0.0	3.437692622421063	0.0
21	0.0	0.0	0.0	3.5617543698471557	0.0
22	0.0	0.0	0.0	3.695644200594029	0.0
23	0.0	0.0	0.0	3.8413206095263326	0.0
24	0.0	0.0	0.0	3.963388253090238	0.0
25	0.0	0.0	0.0	4.061918349280823	0.0
26	0.0	0.0	0.0	4.164044954222853	0.0
27	0.0	0.0	0.0	4.274788936569337	0.0
28	0.0	0.0	0.0	4.376879932513828	0.0
29	3.560899753906218E-5	0.0	0.0	4.504253316711053	0.0
30	3.560899753906218E-5	0.0	0.0	4.648327320754099	0.0
31	3.560899753906218E-5	0.0	0.0	4.776092403924254	0.0
32	3.560899753906218E-5	0.0	0.0	4.89662886059398	0.0
33	3.560899753906218E-5	0.0	0.0	5.020049646064369	0.0
34	3.560899753906218E-5	0.0	0.0	5.155826753680813	0.0
35	3.560899753906218E-5	0.0	0.0	5.317028685540148	0.0
36	3.560899753906218E-5	0.0	0.0	5.446004474626631	0.0
37	3.560899753906218E-5	0.0	0.0	5.577508502538388	0.0
38	3.560899753906218E-5	0.0	0.0	5.727279946187683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCGA	20	7.858425E-4	44.000004	14
ATACGCC	20	7.858425E-4	44.000004	13
CGAACTA	25	4.4444874E-5	44.0	30
CGCTAAT	35	1.4470788E-7	44.0	24
AACGCGT	25	4.4444874E-5	44.0	14
TATTACG	25	4.4444874E-5	44.0	1
TATAGCG	150	0.0	44.0	1
CGCAACT	30	2.5290283E-6	44.0	13
ATCGAGT	30	2.5290283E-6	44.0	30
TAATCCG	55	1.8189894E-12	44.0	1
CGTTTTT	12230	0.0	41.625507	1
TTACGAG	165	0.0	41.333332	1
TCACGAC	440	0.0	41.0	25
ACCCACG	65	0.0	40.615383	25
CGGTCTA	440	0.0	40.0	31
GTGCGTA	55	7.8216544E-11	40.0	16
CGGGTAT	270	0.0	39.925926	6
CATGACG	105	0.0	39.809525	1
CGAATAT	465	0.0	39.741936	14
CGTAAAC	50	1.3496901E-9	39.6	43
>>END_MODULE