FastQCFastQC Report
Thu 26 May 2016
SRR1545810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545810_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2109127
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140780.6674799573472816No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA127490.6044681045759691No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA30860.1463164617398573No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21710.10293358342100783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACG1300.044.0000041
GTTACGT302.5287263E-644.00000444
CGTAGCA302.5287263E-644.00000425
CTATTAG1200.044.0000041
TACTACC600.044.00000426
ATCGCAC302.5287263E-644.00000443
AGCCGAC207.85798E-444.024
ATCTCGA254.4441098E-544.043
ACCGGAT207.85798E-444.016
CGAGTTA351.4468606E-744.029
ATCTACG502.7284841E-1144.01
GATCCGT207.85798E-444.030
CGCGACA207.85798E-444.026
TATGCGA351.4468606E-744.039
CCGTCGA207.85798E-444.035
ACAATCG207.85798E-444.01
TCCGTTA351.4468606E-744.034
CGTACTG207.85798E-444.019
ACTCGAT254.4441098E-544.025
TCGTAAT408.3182385E-944.032
Produced by FastQC (version 0.11.5)