##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545810_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2109127
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719489627699044	31.0	31.0	33.0	30.0	34.0
2	32.14258174116589	33.0	31.0	34.0	30.0	34.0
3	32.18667723660074	33.0	31.0	34.0	30.0	34.0
4	35.86640823430737	37.0	35.0	37.0	35.0	37.0
5	35.85727507162916	37.0	35.0	37.0	35.0	37.0
6	35.923329415440605	37.0	35.0	37.0	35.0	37.0
7	36.101468522284335	37.0	35.0	37.0	35.0	37.0
8	36.04987893095105	37.0	35.0	37.0	35.0	37.0
9	37.804869977009446	39.0	38.0	39.0	35.0	39.0
10	37.59292114699589	39.0	37.0	39.0	35.0	39.0
11	37.30644148029019	39.0	37.0	39.0	35.0	39.0
12	36.38797853329837	38.0	35.0	39.0	33.0	39.0
13	36.08802646782294	38.0	35.0	39.0	33.0	39.0
14	37.04319275226195	39.0	35.0	41.0	33.0	41.0
15	37.27361557649208	39.0	35.0	41.0	33.0	41.0
16	37.37623433771414	39.0	35.0	41.0	33.0	41.0
17	37.323470800952244	38.0	35.0	41.0	33.0	41.0
18	37.245853379146915	38.0	35.0	41.0	33.0	41.0
19	37.13989816639776	38.0	35.0	41.0	33.0	41.0
20	36.96821196637282	38.0	35.0	41.0	33.0	41.0
21	36.831568701173516	38.0	35.0	41.0	33.0	41.0
22	36.81745195998155	38.0	35.0	41.0	33.0	41.0
23	36.80905938807857	38.0	35.0	41.0	33.0	41.0
24	36.773504393049826	38.0	35.0	41.0	33.0	41.0
25	36.68245534763909	38.0	35.0	41.0	33.0	41.0
26	36.5638181105263	37.0	35.0	41.0	32.0	41.0
27	36.456009998449595	37.0	35.0	41.0	32.0	41.0
28	36.43441291112389	37.0	35.0	41.0	32.0	41.0
29	36.423336290322965	37.0	35.0	41.0	32.0	41.0
30	36.326445965558264	37.0	35.0	40.0	32.0	41.0
31	36.15228101484643	37.0	35.0	40.0	31.0	41.0
32	35.92098247284303	37.0	35.0	40.0	31.0	41.0
33	35.71978927774382	37.0	35.0	40.0	30.0	41.0
34	35.550432477513205	37.0	35.0	40.0	30.0	41.0
35	35.33294723361846	36.0	35.0	40.0	29.0	41.0
36	35.15057556989219	36.0	35.0	40.0	27.0	41.0
37	35.05583163081218	36.0	34.0	40.0	27.0	41.0
38	34.99420992666634	36.0	34.0	40.0	27.0	41.0
39	34.97176604348624	36.0	34.0	40.0	27.0	41.0
40	34.78509307405386	36.0	34.0	40.0	25.0	41.0
41	34.72246384404543	36.0	34.0	40.0	25.0	41.0
42	34.705088882746274	36.0	34.0	40.0	25.0	41.0
43	34.61084515062393	35.0	34.0	40.0	25.0	41.0
44	34.47335319305096	35.0	34.0	40.0	24.0	41.0
45	34.36197488344704	35.0	34.0	40.0	24.0	41.0
46	34.32275202014862	35.0	34.0	40.0	24.0	41.0
47	34.28838614270264	35.0	34.0	40.0	24.0	41.0
48	34.232992133712195	35.0	34.0	40.0	24.0	41.0
49	34.19154844634771	35.0	34.0	40.0	24.0	41.0
50	34.00790279580129	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	7.0
12	9.0
13	15.0
14	36.0
15	46.0
16	118.0
17	306.0
18	670.0
19	1473.0
20	2580.0
21	4532.0
22	6491.0
23	9700.0
24	14431.0
25	21219.0
26	30494.0
27	38429.0
28	40668.0
29	42241.0
30	45572.0
31	53579.0
32	67003.0
33	90241.0
34	186909.0
35	405569.0
36	114977.0
37	144883.0
38	244189.0
39	542734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.645592228443334	23.419263041059168	28.82543346133258	15.109711269164919
2	32.49315949205525	25.574420127379717	29.91716477955097	12.015255601014069
3	29.7201638402998	24.060950336323987	33.127165884273445	13.091719939102767
4	27.168397161479607	25.704426523390957	32.293029296007305	14.83414701912213
5	22.6314015229998	32.534740677066864	30.706021970227493	14.127835829705845
6	21.430478107766863	40.35133967750638	27.760016347996114	10.458165866730642
7	84.2080159231758	5.563344454838424	8.199790719098472	2.0288489028873085
8	86.27128665082758	4.044943713678693	7.7312082202731265	1.95256141522061
9	81.7849280768773	5.569555555450193	9.932498137855141	2.71301822981736
10	53.59174672743746	21.855535489328048	14.549432063597878	10.003285719636608
11	46.95900246879396	19.249291294455002	21.1006259935983	12.691080243152737
12	42.7275360848351	19.94621471348098	25.09905757216137	12.227191629522546
13	20.900969927368056	40.463234314481774	25.139026715792838	13.496769042357334
14	14.339771858214323	42.22021717990429	28.683716058824338	14.756294903057046
15	13.334332166815937	22.912228614018975	49.84441430032426	13.909024918840828
16	15.31339743884555	16.560927815157644	48.9834893773585	19.142185368638305
17	15.679947200903502	17.627245775147728	30.18305678131284	36.50975024263594
18	21.277476415597544	21.328777261871856	36.09867969069667	21.29506663183393
19	27.986365922962435	22.782269630989504	28.066399036188905	21.16496540985915
20	29.850549540165193	21.221576510091616	28.121350682059447	20.806523267683737
21	21.755636336740274	26.723473740557115	30.45236251776209	21.068527404940525
22	21.891711594417977	23.64319455395526	27.812502518814657	26.652591332812108
23	18.262484904891927	29.42748350383832	26.416901400437244	25.893130190832508
24	18.832531184703434	23.206378752915306	40.520746261367854	17.440343801013405
25	16.813876072896512	23.928383639297206	36.87710602538396	22.38063426242232
26	16.595159987995032	32.19047501644045	28.937802228125665	22.27656276743885
27	17.916796854812443	33.35787745356254	29.884212757221352	18.841112934403665
28	14.601491517580497	27.520865267952093	39.152075716635366	18.725567497832042
29	15.097905436704382	22.812424287394737	39.55902133916071	22.530648936740178
30	17.17630090554054	30.167789801183144	33.82939955725758	18.826509736018743
31	28.809787177348735	26.421690111595936	25.836613916563582	18.93190879449175
32	29.13195838847068	25.912853991248515	28.01533525482344	16.939852365457366
33	27.364070537241236	27.46036630321455	26.264516077030926	18.911047082513285
34	19.241894869299003	26.906487850186355	29.768714733631498	24.08290254688314
35	20.638918377129496	25.479736402786557	32.45333258736909	21.428012632714864
36	30.40523401388347	24.46196933612817	26.70213789876096	18.4306587512274
37	20.350789686917857	31.161660725029833	30.396984155055623	18.090565432996687
38	20.00140342426037	32.481732963448856	25.77810629706035	21.738757315230426
39	21.077346219549607	30.895484245377354	28.593015024699792	19.43415451037325
40	24.260890880444848	26.09397158160699	26.110708364171526	23.534429173776637
41	17.274445777802853	25.100479961614454	28.24818040829215	29.376893852290547
42	22.2891272076077	25.393207711057702	25.166431419255453	27.151233662079143
43	22.704654579833267	26.082260575109988	26.026645147494676	25.186439697562076
44	19.360901453539782	30.067843235613594	28.299481254566462	22.271774056280158
45	17.400279831418402	37.8767613330065	23.02649390008283	21.69646493549227
46	21.772230880359505	32.92385901844697	26.725844389645577	18.578065711547953
47	21.884315169261974	27.031658122057134	28.611980217407485	22.472046491273403
48	23.38450932542232	24.098596243848757	32.332382070875774	20.18451235985315
49	21.584997015352798	24.51199003189471	32.71922458913095	21.183788363621538
50	19.761446323526275	32.382687244532924	27.87195839795328	19.98390803398752
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1408.0
1	3167.0
2	4926.0
3	15765.0
4	26604.0
5	19380.0
6	12156.0
7	12157.0
8	12158.0
9	12224.0
10	12290.0
11	11962.0
12	11634.0
13	10982.5
14	10331.0
15	9465.0
16	8599.0
17	7549.5
18	6500.0
19	6950.5
20	7401.0
21	7272.0
22	7143.0
23	7283.0
24	7423.0
25	8933.0
26	10443.0
27	14863.5
28	19284.0
29	27275.0
30	35266.0
31	35245.5
32	35225.0
33	39954.0
34	44683.0
35	46111.5
36	47540.0
37	54358.5
38	61177.0
39	79576.5
40	97976.0
41	132531.0
42	167086.0
43	197657.0
44	228228.0
45	234702.0
46	241176.0
47	217843.5
48	194511.0
49	180828.0
50	167145.0
51	151392.0
52	135639.0
53	127818.0
54	119997.0
55	107744.5
56	95492.0
57	88869.5
58	82247.0
59	74274.0
60	66301.0
61	57675.0
62	49049.0
63	39651.0
64	30253.0
65	24885.0
66	19517.0
67	15466.0
68	11415.0
69	9595.0
70	7775.0
71	6588.5
72	5402.0
73	4244.5
74	3087.0
75	2586.0
76	2085.0
77	1666.0
78	1247.0
79	928.5
80	610.0
81	458.5
82	307.0
83	259.5
84	212.0
85	150.5
86	89.0
87	63.0
88	37.0
89	28.0
90	19.0
91	16.5
92	14.0
93	14.0
94	14.0
95	10.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2109127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.546786368420017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92256920625306	11.771633266270474
2	7.6038963016014804	2.2122451013403164
3	2.461443350730997	1.0741827174315528
4	1.2249983670129758	0.7127915818640456
5	0.7167671366342638	0.5213329206261379
6	0.5058022800520401	0.4414678627546069
7	0.3504458497434604	0.35685026369422773
8	0.30601240530130497	0.3561197668803555
9	0.24148800846282886	0.3161587022679579
>10	2.9503709778035074	10.25290124226386
>50	0.9202339282457556	9.533288037243082
>100	1.7590573624887558	57.26905493792492
>500	0.032300472460913734	2.972778581476543
>1k	0.00395515989317311	0.9229551423870368
>5k	0.0	0.0
>10k+	6.591933155288517E-4	1.2862398755749112
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14078	0.6674799573472816	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	12749	0.6044681045759691	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3086	0.1463164617398573	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2171	0.10293358342100783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03271495742077172	0.0
2	0.0	0.0	0.0	0.10843348930623903	0.0
3	0.0	0.0	0.0	0.15935503172639676	0.0
4	0.0	0.0	0.0	0.23123311208855607	0.0
5	0.0	0.0	0.0	0.3955665069007224	0.0
6	0.0	0.0	0.0	0.5383269950078872	0.0
7	0.0	0.0	0.0	0.6407390356294335	0.0
8	0.0	0.0	0.0	0.8853426085769136	0.0
9	0.0	0.0	0.0	1.0014570009297685	0.0
10	0.0	0.0	0.0	1.2267634902971702	0.0
11	0.0	0.0	0.0	1.4137602903950308	0.0
12	0.0	0.0	0.0	1.5937399691910445	0.0
13	0.0	0.0	0.0	1.659217297014357	0.0
14	0.0	0.0	0.0	1.6845358292791284	0.0
15	0.0	0.0	0.0	1.7221343238221312	0.0
16	0.0	0.0	0.0	1.813309487764369	0.0
17	0.0	0.0	0.0	1.9193249150003768	0.0
18	0.0	0.0	0.0	2.0942314047470827	0.0
19	0.0	0.0	0.0	2.163312119184857	0.0
20	0.0	0.0	0.0	2.2327721374767853	0.0
21	0.0	0.0	0.0	2.327929991887639	0.0
22	0.0	0.0	0.0	2.418773264957492	0.0
23	0.0	0.0	0.0	2.530715314914654	0.0
24	0.0	0.0	0.0	2.6092786257062754	0.0
25	0.0	0.0	0.0	2.6731913251312034	0.0
26	0.0	0.0	0.0	2.7419875616783624	0.0
27	0.0	0.0	0.0	2.8075597154652137	0.0
28	0.0	0.0	0.0	2.8800541645903732	0.0
29	0.0	0.0	0.0	2.956531304184148	0.0
30	0.0	0.0	0.0	3.0552451322276943	0.0
31	0.0	0.0	0.0	3.144002234099701	0.0
32	0.0	0.0	0.0	3.2258844536151687	0.0
33	0.0	0.0	0.0	3.305301198078636	0.0
34	0.0	0.0	0.0	3.396239297112028	0.0
35	0.0	0.0	0.0	3.5176639434230372	0.0
36	0.0	0.0	0.0	3.61225284205266	0.0
37	0.0	0.0	0.0	3.702716811268359	0.0
38	0.0	0.0	0.0	3.795598842554289	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGACG	130	0.0	44.000004	1
GTTACGT	30	2.5287263E-6	44.000004	44
CGTAGCA	30	2.5287263E-6	44.000004	25
CTATTAG	120	0.0	44.000004	1
TACTACC	60	0.0	44.000004	26
ATCGCAC	30	2.5287263E-6	44.000004	43
AGCCGAC	20	7.85798E-4	44.0	24
ATCTCGA	25	4.4441098E-5	44.0	43
ACCGGAT	20	7.85798E-4	44.0	16
CGAGTTA	35	1.4468606E-7	44.0	29
ATCTACG	50	2.7284841E-11	44.0	1
GATCCGT	20	7.85798E-4	44.0	30
CGCGACA	20	7.85798E-4	44.0	26
TATGCGA	35	1.4468606E-7	44.0	39
CCGTCGA	20	7.85798E-4	44.0	35
ACAATCG	20	7.85798E-4	44.0	1
TCCGTTA	35	1.4468606E-7	44.0	34
CGTACTG	20	7.85798E-4	44.0	19
ACTCGAT	25	4.4441098E-5	44.0	25
TCGTAAT	40	8.3182385E-9	44.0	32
>>END_MODULE