FastQCFastQC Report
Thu 26 May 2016
SRR1545809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545809_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1959445
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA95010.4848821987858807No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94870.48416771075483106No Hit
TTAGGAGGGACTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATG20440.10431525253324282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC650.044.00000421
ATCCGAT750.044.00000426
ACTACGG1300.044.0000042
ACACTCG650.044.0000041
GCATAAC351.4467878E-744.09
TCACGAC2000.044.025
TCTGCGA207.857844E-444.040
TCGCCTA207.857844E-444.012
AACGTCG254.4439927E-544.016
AATCCGT254.4439927E-544.035
GATACGA351.4467878E-744.018
ATCTCGA302.5286336E-644.042
TCGTTAG1250.044.01
TCGTTAC254.4439927E-544.040
AAATCGT551.8189894E-1244.012
CGAACGT351.4467878E-744.037
ATCTACG254.4439927E-544.043
GTCGAAG254.4439927E-544.09
CGAGTAA207.857844E-444.039
CAACGGC351.4467878E-744.024
Produced by FastQC (version 0.11.5)