##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545809_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1959445
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77132759531398	31.0	31.0	33.0	30.0	34.0
2	32.18736734126245	33.0	31.0	34.0	31.0	34.0
3	32.25810573912511	34.0	31.0	34.0	30.0	34.0
4	35.947324369910866	37.0	35.0	37.0	35.0	37.0
5	35.93553735879292	37.0	35.0	37.0	35.0	37.0
6	35.992110010742834	37.0	35.0	37.0	35.0	37.0
7	36.15308722622988	37.0	35.0	37.0	35.0	37.0
8	36.11096305331357	37.0	35.0	37.0	35.0	37.0
9	37.93474019428971	39.0	39.0	39.0	35.0	39.0
10	37.691902043690945	39.0	38.0	39.0	35.0	39.0
11	37.390292659401005	39.0	37.0	39.0	35.0	39.0
12	36.220054147985785	37.0	35.0	39.0	33.0	39.0
13	35.854083681858896	37.0	35.0	39.0	33.0	39.0
14	36.7156939847763	38.0	35.0	41.0	33.0	41.0
15	36.97276830939373	38.0	35.0	41.0	33.0	41.0
16	37.13785587245368	38.0	35.0	41.0	33.0	41.0
17	37.0900617266624	38.0	35.0	41.0	33.0	41.0
18	37.04361541150683	38.0	35.0	41.0	33.0	41.0
19	36.97374715799627	37.0	35.0	41.0	33.0	41.0
20	36.81214068269331	37.0	35.0	41.0	33.0	41.0
21	36.662910671133915	36.0	35.0	41.0	33.0	41.0
22	36.65319414426024	37.0	35.0	41.0	33.0	41.0
23	36.66998971647584	36.0	35.0	41.0	33.0	41.0
24	36.652494966686994	36.0	35.0	41.0	33.0	41.0
25	36.543836137273566	36.0	35.0	41.0	33.0	41.0
26	36.4486392830623	36.0	35.0	40.0	33.0	41.0
27	36.35349040161883	36.0	35.0	40.0	32.0	41.0
28	36.39190944374555	36.0	35.0	40.0	32.0	41.0
29	36.39493274881408	36.0	35.0	40.0	32.0	41.0
30	36.33320353467436	36.0	35.0	40.0	32.0	41.0
31	36.11490600654777	36.0	35.0	40.0	32.0	41.0
32	35.91637223805721	36.0	35.0	40.0	31.0	41.0
33	35.75870106075955	36.0	35.0	40.0	31.0	41.0
34	35.58019132968774	36.0	35.0	40.0	30.0	41.0
35	35.39518690241369	36.0	35.0	40.0	30.0	41.0
36	35.21537986521694	36.0	35.0	40.0	29.0	41.0
37	35.13328161800918	35.0	34.0	40.0	29.0	41.0
38	35.12317977794733	35.0	34.0	40.0	29.0	41.0
39	35.121543600356226	35.0	35.0	40.0	29.0	41.0
40	34.94571881323538	35.0	34.0	40.0	28.0	41.0
41	34.88763093631105	35.0	34.0	40.0	28.0	41.0
42	34.87692484351436	35.0	34.0	40.0	28.0	41.0
43	34.80472072449086	35.0	34.0	40.0	28.0	41.0
44	34.71098244656012	35.0	34.0	40.0	27.0	41.0
45	34.59455815294637	35.0	34.0	40.0	27.0	41.0
46	34.56923210398863	35.0	34.0	40.0	27.0	41.0
47	34.533852698085425	35.0	34.0	40.0	27.0	41.0
48	34.516462569758275	35.0	34.0	40.0	27.0	41.0
49	34.507438075577525	35.0	34.0	40.0	27.0	41.0
50	34.2957929413686	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	10.0
13	13.0
14	14.0
15	58.0
16	81.0
17	204.0
18	436.0
19	958.0
20	1852.0
21	3021.0
22	4846.0
23	7336.0
24	11477.0
25	17252.0
26	25538.0
27	31520.0
28	33484.0
29	35460.0
30	39791.0
31	48704.0
32	63068.0
33	88419.0
34	193315.0
35	439136.0
36	98445.0
37	122637.0
38	211901.0
39	480464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.749477530627296	23.731056498141058	29.927198773121983	13.59226719810967
2	31.021437192674455	25.46838518049754	30.623722533676627	12.886455093151378
3	28.486944007104054	23.36554483539982	33.738073791303144	14.409437366192979
4	25.541722273398843	26.57089124726644	32.24356896978481	15.643817509549898
5	22.201286588804482	33.299990558551016	30.905894271081863	13.592828581562635
6	19.792900540714335	40.39715327554486	28.338228426927014	11.471717756813792
7	86.44483514464555	4.914707991293453	6.970290056623177	1.6701668074378204
8	89.08915534756015	3.186718688199975	6.147761228306995	1.5763647359328792
9	85.46996726113773	4.3664915320409605	7.854315890468985	2.3092253163523346
10	59.3392516758572	20.07726677707208	12.466846479487813	8.116635067582912
11	52.819752531966955	18.090632806738643	19.02589763938258	10.063717021911817
12	48.82964308771106	20.178979251777925	21.465159777385946	9.526217883125069
13	20.584604313976662	47.63068113675046	20.015412527526927	11.769302021745954
14	12.951677643414333	48.30612750038914	25.91631814110628	12.825876715090242
15	10.68481126033137	23.07561579937176	54.76734483488948	11.4722281054074
16	14.110526194917439	16.334370191559344	52.73722916438073	16.817874449142487
17	14.265570097655203	16.419037023238722	29.35076003664303	39.96463284246305
18	20.459007525090012	21.314862116568722	38.0338310082702	20.192299350071067
19	29.4843692984493	23.108686388237484	26.56150083314408	20.84544348016913
20	32.8688991015313	21.751516373258756	26.103156761225755	19.276427763984188
21	21.28332257348382	29.230675012567335	28.930283830370335	20.555718583578514
22	22.464830602543067	24.692706353074467	25.185090676186366	27.6573723681961
23	16.783987302526988	32.336656553258706	25.64547614247912	25.233880001735187
24	17.435447282266153	21.93697705217549	44.63539420601242	15.992181459545943
25	14.436741015950947	25.153602167960827	40.93174342734805	19.477913388740177
26	14.784798756790826	36.49987624046605	28.801675984781404	19.913649017961717
27	15.700262064002818	37.336898968840664	29.285282312083265	17.67755665507325
28	12.506755739507872	28.994536718305437	41.87037656070979	16.628330981476896
29	13.37225591940575	23.906463309763733	41.394323392593314	21.326957378237203
30	16.41444388589626	32.446075291728015	33.22772519769629	17.911755624679436
31	29.8052764941093	26.04191492999293	25.702839324400532	18.449969251497237
32	31.915976207548567	25.78755719093927	27.118546323065974	15.17792027844619
33	29.73974773469018	27.242867240468605	24.87423734782043	18.14314767702079
34	18.113343319154147	26.66801058462983	30.371151014700594	24.84749508151543
35	18.756433581958156	25.695235130355787	34.07709836203619	21.471232925649865
36	32.04672751723064	24.45202595632947	26.108566456317988	17.392680070121898
37	18.763986741143537	34.389482736182956	30.031462990795866	16.81506753187765
38	19.695730168491586	34.35263556772453	24.819017629992164	21.132616633791713
39	18.910303682930625	33.93093452482718	28.3852315323982	18.773530259843987
40	25.065464965844917	25.19483833432426	25.688447494060817	24.051249205770002
41	15.355827798177547	24.86152966783962	27.36999507513607	32.41264745884676
42	21.195950894258324	25.489564647132223	25.12175641571976	28.192728042889698
43	21.809696112929934	26.37353944611867	26.20971754757087	25.607046893380524
44	18.56566527766791	31.3920013064924	29.626654486346897	20.41567892949279
45	15.799933144334238	41.53196440828908	21.143640163413618	21.52446228396306
46	20.89290589937457	34.883296035356956	26.61748607386275	17.60631199140573
47	21.385239187627107	27.090630254995673	27.208265605822056	24.31586495155516
48	22.689894332323693	23.511606602890105	32.75621413206291	21.042284932723295
49	20.386435955079115	23.64725725907081	34.562950223149926	21.40335656270015
50	17.85985317270962	36.11583892377689	26.105198155600185	19.919109747913314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1301.0
1	2266.5
2	3232.0
3	10579.5
4	17927.0
5	13406.0
6	8885.0
7	9253.0
8	9621.0
9	9961.5
10	10302.0
11	10036.0
12	9770.0
13	8973.5
14	8177.0
15	8196.0
16	8215.0
17	7219.0
18	6223.0
19	5936.0
20	5649.0
21	5866.5
22	6084.0
23	7115.5
24	8147.0
25	9836.5
26	11526.0
27	13920.0
28	16314.0
29	18385.0
30	20456.0
31	25935.5
32	31415.0
33	37794.5
34	44174.0
35	51082.0
36	57990.0
37	63228.5
38	68467.0
39	88155.5
40	107844.0
41	146219.5
42	184595.0
43	216136.0
44	247677.0
45	240742.5
46	233808.0
47	204520.5
48	175233.0
49	166619.0
50	158005.0
51	151491.5
52	144978.0
53	126663.5
54	108349.0
55	93617.5
56	78886.0
57	65985.0
58	53084.0
59	50208.0
60	47332.0
61	38756.5
62	30181.0
63	23203.0
64	16225.0
65	12425.0
66	8625.0
67	6535.0
68	4445.0
69	3414.0
70	2383.0
71	2091.5
72	1800.0
73	1410.5
74	1021.0
75	792.5
76	564.0
77	402.5
78	241.0
79	195.5
80	150.0
81	102.5
82	55.0
83	42.5
84	30.0
85	24.5
86	19.0
87	20.0
88	21.0
89	16.5
90	12.0
91	7.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1959445.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.085761284260546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6256362455252	9.744221930550424
2	8.273216118237595	1.9997623011623247
3	2.5250870801816587	0.9155279901913798
4	1.2349723235773826	0.5970232278169927
5	0.725673244684372	0.4385156802815056
6	0.48112321920887235	0.34888442254040336
7	0.32798660443009037	0.2774777463904085
8	0.27430660986728284	0.26521633644406145
9	0.20734829021107357	0.22553657443715514
>10	2.397593253792749	6.787379172331652
>50	0.8199517741257099	7.176768921085378
>100	1.9742901310820091	60.66836592831679
>500	0.12769040648789115	8.923206004109922
>1k	0.004270582156785657	0.6520816298365673
>5k	8.541164313571315E-4	0.9800321345050451
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	9501	0.4848821987858807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9487	0.48416771075483106	No Hit
TTAGGAGGGACTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATG	2044	0.10431525253324282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02577260397714659	0.0
2	0.0	0.0	0.0	0.09579243102000821	0.0
3	0.0	0.0	0.0	0.13784515513321374	0.0
4	0.0	0.0	0.0	0.19439177930485418	0.0
5	0.0	0.0	0.0	0.3295831217513122	0.0
6	0.0	0.0	0.0	0.4472184725776942	0.0
7	0.0	0.0	0.0	0.52647560916484	0.0
8	0.0	0.0	0.0	0.7283695127957152	0.0
9	0.0	0.0	0.0	0.8094639043198457	0.0
10	0.0	0.0	0.0	0.9459311182503208	0.0
11	0.0	0.0	0.0	1.0683637458566073	0.0
12	0.0	0.0	0.0	1.184417016042808	0.0
13	0.0	0.0	0.0	1.225602147546882	0.0
14	0.0	0.0	0.0	1.239279489855546	0.0
15	0.0	0.0	0.0	1.2679100459568908	0.0
16	0.0	0.0	0.0	1.3375726289842276	0.0
17	0.0	0.0	0.0	1.4249953430690834	0.0
18	0.0	0.0	0.0	1.5866227426643769	0.0
19	0.0	0.0	0.0	1.6379638111812274	0.0
20	0.0	0.0	0.0	1.6978277012113123	0.0
21	0.0	0.0	0.0	1.7711647941126187	0.0
22	0.0	0.0	0.0	1.8417460046084477	0.0
23	0.0	0.0	0.0	1.9314142525051736	0.0
24	0.0	0.0	0.0	1.996432663330688	0.0
25	0.0	0.0	0.0	2.0483861501598666	0.0
26	0.0	0.0	0.0	2.11034246942374	0.0
27	0.0	0.0	0.0	2.1643883854867068	0.0
28	0.0	0.0	0.0	2.2228743343140533	0.0
29	0.0	0.0	0.0	2.289321721201667	0.0
30	0.0	0.0	0.0	2.382970688128526	0.0
31	0.0	0.0	0.0	2.4732003194782197	0.0
32	0.0	0.0	0.0	2.548170527879068	0.0
33	0.0	0.0	0.0	2.61895587781234	0.0
34	0.0	0.0	0.0	2.697702665805879	0.0
35	0.0	0.0	0.0	2.815593190929064	0.0
36	0.0	0.0	0.0	2.9080683560906277	0.0
37	0.0	0.0	0.0	3.001564218439405	0.0
38	0.0	0.0	0.0	3.0816889476356826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAC	65	0.0	44.000004	21
ATCCGAT	75	0.0	44.000004	26
ACTACGG	130	0.0	44.000004	2
ACACTCG	65	0.0	44.000004	1
GCATAAC	35	1.4467878E-7	44.0	9
TCACGAC	200	0.0	44.0	25
TCTGCGA	20	7.857844E-4	44.0	40
TCGCCTA	20	7.857844E-4	44.0	12
AACGTCG	25	4.4439927E-5	44.0	16
AATCCGT	25	4.4439927E-5	44.0	35
GATACGA	35	1.4467878E-7	44.0	18
ATCTCGA	30	2.5286336E-6	44.0	42
TCGTTAG	125	0.0	44.0	1
TCGTTAC	25	4.4439927E-5	44.0	40
AAATCGT	55	1.8189894E-12	44.0	12
CGAACGT	35	1.4467878E-7	44.0	37
ATCTACG	25	4.4439927E-5	44.0	43
GTCGAAG	25	4.4439927E-5	44.0	9
CGAGTAA	20	7.857844E-4	44.0	39
CAACGGC	35	1.4467878E-7	44.0	24
>>END_MODULE