FastQCFastQC Report
Thu 26 May 2016
SRR1545808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545808_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences731003
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.6514337150463131No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19160.26210562747348504No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14930.20423992787991294No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14050.19220167359094287No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8130.11121705382878046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT254.441246E-544.032
ACACGAT207.854599E-444.043
GTCGAAA254.441246E-544.042
GGTCGAA254.441246E-544.041
ACGTTAG351.4451143E-744.01
GACCGGT207.854599E-444.026
TACGGGT302.5264471E-644.04
TAACGCG207.854599E-444.01
ACTTACG408.305506E-944.01
GCGTTAG351.4451143E-744.01
TTAACGG502.7284841E-1144.02
CTCGATC254.441246E-544.016
AGTTACC351.4451143E-744.030
TTACGAG454.783942E-1044.01
ACGTATC254.441246E-544.033
CCGGTGT207.854599E-444.029
GACGCTT207.854599E-444.036
CCGGTAC207.854599E-444.014
CCAAACG254.441246E-544.029
TATCGAA207.854599E-444.025
Produced by FastQC (version 0.11.5)