FastQCFastQC Report
Thu 26 May 2016
SRR1545807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545807_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4577309
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314050.6861018122219845No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA132180.28877228957013823No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA97410.21281062737953677No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA94420.20627840506288736No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA64070.13997307151428928No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA207.8589446E-444.00000428
TAATCGC408.3218765E-944.00000425
ATCGTTC600.044.014
CGGTAGT1200.044.012
ACCTAGT650.043.99999614
CGGTCTA5850.043.62393631
ACGTAGG2350.043.0638282
TCGTAAG2800.042.4285741
TAATGCG1350.042.370371
CTATGCG850.041.4117661
CGTTTTT175150.041.186411
ACGAACT700.040.85714310
CGACGGT6250.040.83228
CGTATGG6200.040.806452
CAATCGT650.040.61538312
TATCGCG650.040.61538332
TACGTAG650.040.6153831
CACGACG6300.040.50793526
CGTAATC603.6379788E-1240.33333230
AGGGCGA31700.040.321776