##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545807_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4577309
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66235423476982	31.0	31.0	33.0	30.0	34.0
2	32.07965837569629	33.0	31.0	34.0	30.0	34.0
3	32.162627430221555	33.0	31.0	34.0	30.0	34.0
4	35.8554056542829	37.0	35.0	37.0	35.0	37.0
5	35.83170395531523	37.0	35.0	37.0	35.0	37.0
6	35.88243769428719	37.0	35.0	37.0	35.0	37.0
7	36.04454975619955	37.0	35.0	37.0	35.0	37.0
8	35.978927575132026	37.0	35.0	37.0	35.0	37.0
9	37.69303863907811	39.0	38.0	39.0	35.0	39.0
10	37.50901457603146	39.0	37.0	39.0	35.0	39.0
11	37.29431200733881	39.0	37.0	39.0	34.0	39.0
12	36.56318810899592	39.0	35.0	39.0	33.0	39.0
13	36.32949337700382	38.0	35.0	39.0	33.0	39.0
14	37.35957764704109	39.0	35.0	41.0	33.0	41.0
15	37.50588478951279	39.0	35.0	41.0	33.0	41.0
16	37.56155461647881	39.0	35.0	41.0	33.0	41.0
17	37.46519865711491	39.0	35.0	41.0	33.0	41.0
18	37.401246889821074	39.0	35.0	41.0	33.0	41.0
19	37.32363578687827	39.0	35.0	41.0	33.0	41.0
20	37.18594265757457	39.0	35.0	41.0	33.0	41.0
21	37.07951069067	38.0	35.0	41.0	33.0	41.0
22	37.074045689290365	39.0	35.0	41.0	33.0	41.0
23	37.06775159815516	38.0	35.0	41.0	33.0	41.0
24	37.0331159639867	38.0	35.0	41.0	33.0	41.0
25	36.919018576198376	38.0	35.0	41.0	32.0	41.0
26	36.81040366730758	38.0	35.0	41.0	32.0	41.0
27	36.70901789675987	38.0	35.0	41.0	32.0	41.0
28	36.667489566467985	38.0	35.0	41.0	32.0	41.0
29	36.60944738491546	38.0	35.0	41.0	31.0	41.0
30	36.51555619251398	38.0	35.0	41.0	31.0	41.0
31	36.38839283080954	38.0	35.0	41.0	31.0	41.0
32	36.198973021047955	38.0	35.0	41.0	31.0	41.0
33	36.02856700301422	38.0	35.0	41.0	30.0	41.0
34	35.83223680114233	38.0	35.0	41.0	30.0	41.0
35	35.62179328509393	38.0	35.0	41.0	29.0	41.0
36	35.50024654223694	38.0	35.0	40.0	28.0	41.0
37	35.41266757389549	38.0	35.0	40.0	28.0	41.0
38	35.34913854406596	38.0	35.0	40.0	27.0	41.0
39	35.29806006105334	38.0	35.0	40.0	27.0	41.0
40	35.148523510210914	37.0	34.0	40.0	26.0	41.0
41	35.06865671511362	37.0	34.0	40.0	26.0	41.0
42	35.01763678178598	37.0	34.0	40.0	26.0	41.0
43	34.93499237215578	37.0	34.0	40.0	26.0	41.0
44	34.80103615464894	37.0	34.0	40.0	25.0	41.0
45	34.6981287476987	36.0	34.0	40.0	24.0	41.0
46	34.635086903680744	36.0	34.0	40.0	24.0	41.0
47	34.57932531974573	36.0	34.0	40.0	24.0	41.0
48	34.503521173685236	36.0	34.0	40.0	24.0	41.0
49	34.433888557665654	36.0	34.0	40.0	24.0	41.0
50	34.290133132807945	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	11.0
11	12.0
12	18.0
13	31.0
14	47.0
15	130.0
16	296.0
17	652.0
18	1599.0
19	3092.0
20	5775.0
21	9309.0
22	13951.0
23	20719.0
24	30063.0
25	43991.0
26	62992.0
27	79522.0
28	86222.0
29	91016.0
30	100510.0
31	118297.0
32	144168.0
33	190564.0
34	365004.0
35	715931.0
36	267516.0
37	346522.0
38	582122.0
39	1297225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.901097784746455	23.371024328923394	27.668352737383472	15.059525148946683
2	30.951941413612232	26.39618605604297	29.566869966611385	13.085002563733408
3	29.53442732400194	24.801865025935545	31.93561981504854	13.72808783501398
4	27.092971001083825	27.098563806813132	30.500540819944643	15.307924372158402
5	22.948985965334654	33.11744957572233	29.572812322698773	14.360752136244242
6	20.62205107848301	41.40764803075344	27.742632188475806	10.227668702287742
7	84.10747450084754	5.155671159626759	8.65930178626787	2.0775525532578203
8	85.05193772148658	4.486216683208409	8.005533382168432	2.456312213136583
9	79.6121913552264	6.410950189292443	10.032379286607043	3.944479168874113
10	47.00338124430752	25.91424349983801	14.968707596537618	12.11366765931686
11	41.91244244161799	21.036333793501814	22.57450829734239	14.476715467537804
12	38.23488866493391	20.68608433470408	26.292107436924184	14.78691956343782
13	21.944902561745337	34.72376455249143	26.716286796456174	16.615046089307057
14	15.729766113670717	37.70339734547088	29.869405801530984	16.69743073932741
15	16.93184794821586	23.10838092861985	44.54800844775828	15.411762675406008
16	18.876309202634122	19.623538633725623	41.95635470535199	19.543797458288264
17	17.904406278885695	20.287072600953966	30.83383708637542	30.974684033784918
18	22.87907589371834	22.68599301467303	33.54477488847705	20.89015620313158
19	27.27036780781022	24.328573841093096	27.606788180566355	20.794270170530325
20	27.89287330175874	23.455637362476512	27.589048499893714	21.062440835871033
21	22.547199675617268	26.342114985027226	30.331926465965047	20.77875887339046
22	22.333012693702784	24.178289034015403	28.1012708558675	25.387427416414315
23	20.69467016537446	27.489055250585004	28.140507883562154	23.67576670047838
24	20.730717546051622	23.694664266712167	36.37213480671722	19.202483380518988
25	18.940779396802792	25.811759704227967	33.32648068985511	21.920980209114134
26	17.789098354513534	30.523064971143526	29.64333410744173	22.04450256690121
27	19.316611572432624	30.331904619067668	29.32083020831672	21.030653600182987
28	16.39445796646021	28.227021597187342	35.96899400936227	19.40952642699018
29	18.155973302217525	25.00875514412507	35.10304853790732	21.732223015750087
30	18.620460187415794	29.256862492787793	31.316435049501795	20.80624227029462
31	27.025944719921686	27.068502476018114	25.869326278824524	20.03622652523568
32	27.715694964006143	26.75307697164426	27.197683180226633	18.33354488412296
33	25.632178207763555	28.03278083258089	26.42841022967862	19.90663072997694
34	19.690695996272044	28.3070904760854	29.99410789177659	22.008105635865963
35	20.996201043014576	26.834849908537965	30.494882473523198	21.67406657492426
36	27.26132319229486	27.274911962465282	26.242798989537302	19.220965855702556
37	20.467353198134536	31.997249912557795	28.12180693940479	19.413589949902878
38	20.61774723969913	32.624845733595876	24.928795499713914	21.828611526991075
39	21.677015906070576	30.08617071733632	26.96545066107619	21.27136271551691
40	22.909989253511178	26.783138302439273	26.749078989423698	23.55779345462585
41	18.218564663211506	26.964314622412427	27.390307274426963	27.426813439949104
42	21.290981229364238	27.56117185883671	24.855236996235124	26.292609915563926
43	22.53874492633117	26.138676676623756	26.456680114888464	24.86589828215661
44	19.865514869107592	29.476052414202318	28.314824277757957	22.343608438932133
45	19.02888793393673	33.87435718235321	25.00705108612943	22.089703797580633
46	21.4467933014791	32.467373297280126	26.38185449136163	19.703978909879144
47	22.059205528838014	27.19425321733796	27.989261812999732	22.757279440824295
48	22.177659406432905	25.374210043499357	30.313968316318608	22.13416223374913
49	21.054597799711576	25.008471134459132	32.22793130199425	21.708999763835042
50	19.583689892904324	30.151536634297578	28.789950601980337	21.474822870817768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3645.0
1	7854.5
2	12064.0
3	37218.0
4	62372.0
5	44155.5
6	25939.0
7	24598.0
8	23257.0
9	22751.5
10	22246.0
11	21628.0
12	21010.0
13	19452.0
14	17894.0
15	17684.5
16	17475.0
17	15309.5
18	13144.0
19	14853.5
20	16563.0
21	17884.0
22	19205.0
23	20135.5
24	21066.0
25	23677.0
26	26288.0
27	35513.0
28	44738.0
29	60004.0
30	75270.0
31	84383.0
32	93496.0
33	92126.5
34	90757.0
35	103741.0
36	116725.0
37	132875.0
38	149025.0
39	180159.0
40	211293.0
41	275854.5
42	340416.0
43	372895.0
44	405374.0
45	428428.5
46	451483.0
47	421698.5
48	391914.0
49	382626.0
50	373338.0
51	349044.5
52	324751.0
53	298136.5
54	271522.0
55	247731.0
56	223940.0
57	212351.5
58	200763.0
59	191087.0
60	181411.0
61	154246.5
62	127082.0
63	105924.5
64	84767.0
65	65552.0
66	46337.0
67	36350.0
68	26363.0
69	22032.0
70	17701.0
71	14544.0
72	11387.0
73	9162.5
74	6938.0
75	5457.0
76	3976.0
77	3148.5
78	2321.0
79	1672.5
80	1024.0
81	770.0
82	516.0
83	374.5
84	233.0
85	174.0
86	115.0
87	101.0
88	87.0
89	59.0
90	31.0
91	23.0
92	15.0
93	14.0
94	13.0
95	13.5
96	14.0
97	9.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4577309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.700402667708744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43783300255731	10.88330299185238
2	8.831547148608758	2.4199150422958993
3	3.0514933569065708	1.2542006318237486
4	1.4518294209079203	0.7956259068505965
5	0.8449549405280494	0.5788111460652086
6	0.5750643136190786	0.47271675938465535
7	0.38422185693328365	0.36847959076045367
8	0.3060150165667221	0.33540231594637243
9	0.24896828892302883	0.3069869228762351
>10	2.3367559410772665	7.423838687337475
>50	0.8520419905398732	8.432258670607432
>100	1.5050046153878278	49.61824777774251
>500	0.16439965567637305	13.546526055205263
>1k	0.009061398344367018	2.0071554892495636
>5k	4.854320541625188E-4	0.56729742621931
>10k+	3.2362136944167925E-4	0.9892345857828946
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31405	0.6861018122219845	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	13218	0.28877228957013823	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9741	0.21281062737953677	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9442	0.20627840506288736	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6407	0.13997307151428928	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05206115645677405	0.0
2	0.0	0.0	0.0	0.17599860529407124	0.0
3	0.0	0.0	0.0	0.2390050573382745	0.0
4	0.0	0.0	0.0	0.3426467385094605	0.0
5	0.0	0.0	0.0	0.626809332732398	0.0
6	0.0	0.0	0.0	0.8313181391074974	0.0
7	0.0	0.0	0.0	0.9887468816284852	0.0
8	0.0	0.0	0.0	1.314964753308112	0.0
9	0.0	0.0	0.0	1.4625405451106752	0.0
10	0.0	0.0	0.0	1.7966232998471372	0.0
11	0.0	0.0	0.0	2.024333511239901	0.0
12	0.0	0.0	0.0	2.290144711663556	0.0
13	0.0	0.0	0.0	2.38006654128004	0.0
14	0.0	0.0	0.0	2.41111098245716	0.0
15	0.0	0.0	0.0	2.4635435361693956	0.0
16	0.0	0.0	0.0	2.570331170563316	0.0
17	0.0	0.0	0.0	2.7031384597369326	0.0
18	0.0	0.0	0.0	2.9043920784023975	0.0
19	0.0	0.0	0.0	2.9933307976367773	0.0
20	0.0	0.0	0.0	3.0885832702140057	0.0
21	0.0	0.0	0.0	3.2061632719136943	0.0
22	0.0	0.0	0.0	3.326867379938737	0.0
23	0.0	0.0	0.0	3.4529676716166637	0.0
24	0.0	0.0	0.0	3.554664978921021	0.0
25	0.0	0.0	0.0	3.6444120333584644	0.0
26	0.0	0.0	0.0	3.7299863303963092	0.0
27	0.0	0.0	0.0	3.8183570302988064	0.0
28	0.0	0.0	0.0	3.9148329291293202	0.0
29	0.0	0.0	0.0	4.011964234881237	0.0
30	2.184689738009822E-5	0.0	0.0	4.132144017369157	0.0
31	2.184689738009822E-5	0.0	0.0	4.255207590311251	0.0
32	2.184689738009822E-5	0.0	0.0	4.363043875779415	0.0
33	2.184689738009822E-5	0.0	0.0	4.474747062083858	0.0
34	4.369379476019644E-5	0.0	0.0	4.58214640960442	0.0
35	4.369379476019644E-5	0.0	0.0	4.719323078254057	0.0
36	4.369379476019644E-5	0.0	0.0	4.841927866351169	0.0
37	4.369379476019644E-5	0.0	0.0	4.962610127478831	0.0
38	4.369379476019644E-5	0.0	0.0	5.082134503045348	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATA	20	7.8589446E-4	44.000004	28
TAATCGC	40	8.3218765E-9	44.000004	25
ATCGTTC	60	0.0	44.0	14
CGGTAGT	120	0.0	44.0	12
ACCTAGT	65	0.0	43.999996	14
CGGTCTA	585	0.0	43.623936	31
ACGTAGG	235	0.0	43.063828	2
TCGTAAG	280	0.0	42.428574	1
TAATGCG	135	0.0	42.37037	1
CTATGCG	85	0.0	41.411766	1
CGTTTTT	17515	0.0	41.18641	1
ACGAACT	70	0.0	40.857143	10
CGACGGT	625	0.0	40.832	28
CGTATGG	620	0.0	40.80645	2
CAATCGT	65	0.0	40.615383	12
TATCGCG	65	0.0	40.615383	32
TACGTAG	65	0.0	40.615383	1
CACGACG	630	0.0	40.507935	26
CGTAATC	60	3.6379788E-12	40.333332	30
AGGGCGA	3170	0.0	40.32177	6
>>END_MODULE