FastQCFastQC Report
Thu 26 May 2016
SRR1545806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545806_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2623404
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123020.46893273014754877No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39250.1496147753071963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA207.8583293E-444.00000421
TATCGGC408.3200575E-944.00000415
GCGTAGT207.8583293E-444.00000429
CGTATAT254.4444063E-544.014
CGACGGT3750.042.2428
CGTTTTT89050.040.8871421
AATCGTC557.8216544E-1140.043
TGTTACG1500.039.61
CATACGA4600.039.21739218
GGGCGAT32900.038.7841957
CAATACG404.1272324E-738.5000041
GCGCGAC4600.038.260879
AACGGGA6750.038.1333354
GACCGAT10850.037.9170539
TCACGAC4300.037.86046625
GTTAGCG2150.037.8604661
TATTACG357.29378E-637.7142871
AGCGATA357.29378E-637.71428743
ACGCGGT700.037.7142873
GCGATTT3450.037.6231889
Produced by FastQC (version 0.11.5)