##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545806_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2623404
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.683282864553078	31.0	31.0	33.0	30.0	34.0
2	32.12589368621836	33.0	31.0	34.0	30.0	34.0
3	32.21803580386399	33.0	31.0	34.0	30.0	34.0
4	35.89990752472742	37.0	35.0	37.0	35.0	37.0
5	35.889715423167765	37.0	35.0	37.0	35.0	37.0
6	35.94264741534281	37.0	35.0	37.0	35.0	37.0
7	36.11026589880933	37.0	35.0	37.0	35.0	37.0
8	36.0566306218943	37.0	35.0	37.0	35.0	37.0
9	37.87679747381646	39.0	38.0	39.0	35.0	39.0
10	37.492957622996684	39.0	37.0	39.0	35.0	39.0
11	37.333136642316624	39.0	37.0	39.0	34.0	39.0
12	36.83992972489178	39.0	35.0	39.0	33.0	39.0
13	36.651440647342156	39.0	35.0	39.0	33.0	39.0
14	37.72074678547414	40.0	35.0	41.0	33.0	41.0
15	37.85988128401115	40.0	35.0	41.0	33.0	41.0
16	37.899019365679095	40.0	35.0	41.0	33.0	41.0
17	37.833584152498055	40.0	35.0	41.0	33.0	41.0
18	37.76588699262485	39.0	36.0	41.0	33.0	41.0
19	37.71957350068842	39.0	36.0	41.0	33.0	41.0
20	37.598249068767146	39.0	35.0	41.0	33.0	41.0
21	37.48786957708382	39.0	35.0	41.0	33.0	41.0
22	37.50372836208224	39.0	35.0	41.0	33.0	41.0
23	37.49432531169427	39.0	35.0	41.0	33.0	41.0
24	37.460373240263415	39.0	35.0	41.0	33.0	41.0
25	37.379702478154336	39.0	35.0	41.0	33.0	41.0
26	37.29187231551069	39.0	35.0	41.0	33.0	41.0
27	37.2109732241012	39.0	35.0	41.0	33.0	41.0
28	37.171760049157506	39.0	35.0	41.0	33.0	41.0
29	37.11546181983408	39.0	35.0	41.0	32.0	41.0
30	37.012566878757525	39.0	35.0	41.0	32.0	41.0
31	36.898553558658904	39.0	35.0	41.0	32.0	41.0
32	36.755760454737434	39.0	35.0	41.0	31.0	41.0
33	36.62059484547557	39.0	35.0	41.0	31.0	41.0
34	36.471950946175276	39.0	35.0	41.0	30.0	41.0
35	36.262621769273814	39.0	35.0	41.0	30.0	41.0
36	36.14404796211335	39.0	35.0	41.0	30.0	41.0
37	36.09629778714982	39.0	35.0	41.0	30.0	41.0
38	36.007569173486054	39.0	35.0	41.0	30.0	41.0
39	35.9493158507039	39.0	35.0	41.0	29.0	41.0
40	35.81949368072931	38.0	35.0	41.0	29.0	41.0
41	35.716466849940005	38.0	35.0	41.0	28.0	41.0
42	35.69936578582635	38.0	35.0	41.0	28.0	41.0
43	35.638391570646384	38.0	35.0	40.0	28.0	41.0
44	35.52351753675759	38.0	35.0	40.0	27.0	41.0
45	35.42337855701981	38.0	35.0	40.0	27.0	41.0
46	35.3763785524456	38.0	35.0	40.0	27.0	41.0
47	35.31745663268029	38.0	35.0	40.0	27.0	41.0
48	35.25435998420373	38.0	34.0	40.0	27.0	41.0
49	35.18701961268642	38.0	34.0	40.0	27.0	41.0
50	35.056896307240514	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	5.0
12	10.0
13	10.0
14	21.0
15	48.0
16	151.0
17	287.0
18	719.0
19	1446.0
20	2723.0
21	4321.0
22	6534.0
23	9942.0
24	14534.0
25	22225.0
26	31742.0
27	39570.0
28	42579.0
29	45132.0
30	50000.0
31	59460.0
32	73877.0
33	97795.0
34	178661.0
35	323740.0
36	155017.0
37	211288.0
38	380122.0
39	871443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.22786120628008	23.695892817118523	29.462065316664916	14.61418065993648
2	30.7788659314387	24.722802892730208	31.17064699146605	13.327684184365046
3	29.480285918600412	24.35549385454928	32.57965604992597	13.584564176924333
4	25.457154140193428	27.182927219749608	32.60389936128785	14.756019278769111
5	22.80064374377717	31.777187196482124	31.060332301086678	14.361836758654023
6	20.197461008674225	41.075716893013805	27.77505866423929	10.951763434072678
7	87.98480904961646	3.6993920875320767	6.708878998431047	1.6069198644204248
8	89.69053184336076	2.5351413659504978	6.273871656824492	1.5004551338642467
9	85.34453709760295	3.771397771749986	8.232281417578077	2.6517837130689745
10	41.514726668099925	24.191851502856593	19.733064369803508	14.560357459239981
11	36.40800273232792	21.767672840325012	24.229436259150326	17.59488816819674
12	34.481231255269876	22.39803705414797	26.465309956072343	16.65542173450982
13	23.695206685664886	31.56845076091978	27.14473256883042	17.591609984584913
14	19.054823427882248	34.091546708017525	29.684676854956383	17.168953009143845
15	18.993300307539364	24.478425739992772	40.10564137281181	16.42263257965605
16	20.688578655822738	21.309794450263855	38.352727982422834	19.648898911490566
17	20.451558357004867	21.551541432429012	30.760797803159555	27.23610240740656
18	22.267443367472183	23.65194228567159	33.920166318264364	20.16044802859186
19	25.68666511143537	24.825722610775923	29.1595194640246	20.3280928137641
20	27.238427630666113	24.4321118668722	28.41979352017455	19.909666982287135
21	23.39288954350912	25.854919791233073	31.583507534485726	19.16868313077208
22	22.891822990282854	23.79827887736696	29.715209704643282	23.594688427706902
23	19.73809600046352	27.918155190736922	29.751345961201555	22.592402847598006
24	19.478204653191046	25.39265778355145	36.663205514667204	18.465932048590304
25	19.201541203718527	26.19463109761211	33.848236870874636	20.75559082779473
26	19.18484533834667	30.056560102828232	29.89604346109101	20.862551097734087
27	18.813838813998913	29.97231840768711	30.812791319979695	20.401051458334287
28	17.13449396280558	27.03174958946468	36.57431337300698	19.259443074722764
29	18.65385583005896	24.63631221115772	35.645367621609175	21.064464337174144
30	19.678898103380188	27.702976743193197	33.12631222640508	19.491812927021535
31	25.0940762459766	26.578292935438082	28.894253420365295	19.433377398220024
32	25.361476920825005	25.90683707122502	29.578174005986114	19.15351200196386
33	23.678320228222567	26.855604397950145	29.040170709505663	20.425904664321624
34	19.996767558485082	26.885946655566585	31.15623823093965	21.961047555008683
35	19.946146304572228	27.1693951827473	31.613887910516258	21.27057060216421
36	24.503164590737835	27.104327049893957	28.77269379782908	19.61981456153913
37	20.48434019312313	30.01127542688812	29.817786356962177	19.686598023026573
38	20.831027169280826	30.133444944049792	28.55195006182807	20.483577824841312
39	20.786619216864807	29.69355844543959	28.000147899446677	21.51967443824893
40	22.873564269933265	26.542347270950263	28.63462890199146	21.949459557125017
41	19.388054603865818	26.64995555392917	28.881598106887086	25.08039173531793
42	21.513956676135283	27.854726149689483	26.89787771917707	23.733439454998162
43	21.427161047250063	27.48875125600174	28.254588313504136	22.82949938324406
44	20.456475632422606	29.086446464212145	29.290075032286296	21.16700287107895
45	18.706802307231367	32.19626104099864	27.019132394400557	22.077804257369433
46	20.935281031819727	30.192680959547214	28.46980487946195	20.40223312917111
47	20.78669545369299	28.496144703598837	29.798803386744854	20.918356455963323
48	21.244955027895056	26.63325968855731	30.697368762112127	21.42441652143551
49	20.62183331274939	27.079702554391165	31.097002215442227	21.20146191741722
50	19.819364459305543	30.638170865028798	29.10371410579537	20.43875056987029
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1043.0
1	2993.0
2	4943.0
3	15670.5
4	26398.0
5	19400.0
6	12402.0
7	12229.0
8	12056.0
9	11976.0
10	11896.0
11	11363.0
12	10830.0
13	10052.5
14	9275.0
15	8755.0
16	8235.0
17	8012.5
18	7790.0
19	7792.0
20	7794.0
21	8475.5
22	9157.0
23	10736.0
24	12315.0
25	15168.0
26	18021.0
27	22837.0
28	27653.0
29	30742.0
30	33831.0
31	41059.5
32	48288.0
33	64308.0
34	80328.0
35	85663.5
36	90999.0
37	98801.0
38	106603.0
39	124098.0
40	141593.0
41	168298.0
42	195003.0
43	216523.5
44	238044.0
45	242641.5
46	247239.0
47	243410.5
48	239582.0
49	235092.5
50	230603.0
51	218597.5
52	206592.0
53	185039.5
54	163487.0
55	146780.5
56	130074.0
57	115093.0
58	100112.0
59	85549.5
60	70987.0
61	60073.5
62	49160.0
63	39657.5
64	30155.0
65	23394.0
66	16633.0
67	12997.0
68	9361.0
69	7663.5
70	5966.0
71	4868.0
72	3770.0
73	3121.0
74	2472.0
75	1847.0
76	1222.0
77	938.0
78	654.0
79	538.0
80	422.0
81	324.0
82	226.0
83	152.0
84	78.0
85	60.5
86	43.0
87	35.5
88	28.0
89	22.0
90	16.0
91	12.5
92	9.0
93	10.0
94	11.0
95	7.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2623404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.803121896030538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27058797992464	11.734521766368378
2	7.180048280782813	2.12574259839625
3	2.338692750743907	1.0385986159987504
4	1.2171491570089186	0.7207042934741533
5	0.7643958167133161	0.5657722225811518
6	0.5638369435173421	0.5007928202623501
7	0.447791886639963	0.46401025173894034
8	0.3754254436517998	0.444597488419915
9	0.3094990801254625	0.4123397348825889
>10	4.010292973548055	14.299678027156235
>50	1.4749331607086684	15.903563563994613
>100	2.0416247756755035	50.64107264197757
>500	0.004681432603318634	0.4296203684462621
>1k	7.802387672197724E-4	0.24535985376001693
>5k	0.0	0.0
>10k+	2.6007958907325743E-4	0.4736257525428727
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12302	0.46893273014754877	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3925	0.1496147753071963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019021088631411706	0.0
2	0.0	0.0	0.0	0.07364477602382248	0.0
3	0.0	0.0	0.0	0.10585483593072206	0.0
4	0.0	0.0	0.0	0.1503390251749254	0.0
5	0.0	0.0	0.0	0.27788323872342957	0.0
6	0.0	0.0	0.0	0.39220036258235486	0.0
7	0.0	0.0	0.0	0.4819692277666726	0.0
8	0.0	0.0	0.0	0.6870462955762818	0.0
9	0.0	0.0	0.0	0.7767008055183265	0.0
10	0.0	0.0	0.0	0.9471663533333028	0.0
11	0.0	0.0	0.0	1.0786367635331806	0.0
12	0.0	0.0	0.0	1.2060666218394116	0.0
13	0.0	0.0	0.0	1.249750324387704	0.0
14	0.0	0.0	0.0	1.2659887687904723	0.0
15	3.8118414091005426E-5	0.0	0.0	1.2908038563637168	0.0
16	3.8118414091005426E-5	0.0	0.0	1.3532037002306927	0.0
17	3.8118414091005426E-5	0.0	0.0	1.4310415018045257	0.0
18	3.8118414091005426E-5	0.0	0.0	1.5449393231084498	0.0
19	3.8118414091005426E-5	0.0	0.0	1.5957892875058513	0.0
20	3.8118414091005426E-5	0.0	0.0	1.6534243296114515	0.0
21	3.8118414091005426E-5	0.0	0.0	1.7329774598193797	0.0
22	3.8118414091005426E-5	0.0	0.0	1.8164567866786816	0.0
23	3.8118414091005426E-5	0.0	0.0	1.9082078093957318	0.0
24	3.8118414091005426E-5	0.0	0.0	1.9819288222477363	0.0
25	3.8118414091005426E-5	0.0	0.0	2.043566297832892	0.0
26	3.8118414091005426E-5	0.0	0.0	2.10871066751442	0.0
27	3.8118414091005426E-5	0.0	0.0	2.177590641776867	0.0
28	3.8118414091005426E-5	0.0	0.0	2.2454795372729475	0.0
29	3.8118414091005426E-5	0.0	0.0	2.330597955938163	0.0
30	3.8118414091005426E-5	0.0	0.0	2.424483609844309	0.0
31	3.8118414091005426E-5	0.0	0.0	2.508344120844521	0.0
32	3.8118414091005426E-5	0.0	0.0	2.5832468045333465	0.0
33	3.8118414091005426E-5	0.0	0.0	2.66222815852991	0.0
34	3.8118414091005426E-5	0.0	0.0	2.749176261071493	0.0
35	3.8118414091005426E-5	0.0	0.0	2.851295492421297	0.0
36	3.8118414091005426E-5	0.0	0.0	2.943351462451075	0.0
37	3.8118414091005426E-5	0.0	0.0	3.0371989979431304	0.0
38	3.8118414091005426E-5	0.0	0.0	3.146293899071588	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGA	20	7.8583293E-4	44.000004	21
TATCGGC	40	8.3200575E-9	44.000004	15
GCGTAGT	20	7.8583293E-4	44.000004	29
CGTATAT	25	4.4444063E-5	44.0	14
CGACGGT	375	0.0	42.24	28
CGTTTTT	8905	0.0	40.887142	1
AATCGTC	55	7.8216544E-11	40.0	43
TGTTACG	150	0.0	39.6	1
CATACGA	460	0.0	39.217392	18
GGGCGAT	3290	0.0	38.784195	7
CAATACG	40	4.1272324E-7	38.500004	1
GCGCGAC	460	0.0	38.26087	9
AACGGGA	675	0.0	38.133335	4
GACCGAT	1085	0.0	37.917053	9
TCACGAC	430	0.0	37.860466	25
GTTAGCG	215	0.0	37.860466	1
TATTACG	35	7.29378E-6	37.714287	1
AGCGATA	35	7.29378E-6	37.714287	43
ACGCGGT	70	0.0	37.714287	3
GCGATTT	345	0.0	37.623188	9
>>END_MODULE