FastQCFastQC Report
Thu 26 May 2016
SRR1545805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545805_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2456353
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146370.5958834092656877No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA82780.33700367984568996No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA77510.3155491087803748No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA52680.21446428913108173No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA38000.15470089193206352No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA34790.1416327376399076No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAAC26010.10588868945139399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC207.8582315E-444.0000048
ATAGCCG207.8582315E-444.00000415
ACGATTC207.8582315E-444.00000415
CCCGTAG207.8582315E-444.00000442
TACGAAC207.8582315E-444.00000434
TCTAACG408.3200575E-944.00000419
CGTAATA207.8582315E-444.00000427
CGCGTAC207.8582315E-444.00000426
GTGCGTA551.8189894E-1244.00000416
TGTAACG551.8189894E-1244.0000041
TACCGGT408.3200575E-944.00000417
CTATCGA302.5288973E-644.011
GTAATCG254.4443237E-544.041
CTACGTC302.5288973E-644.016
CGTAGCA302.5288973E-644.015
ACGTGTA351.4469879E-744.022
GTCGTAC351.4469879E-744.039
TATTACG254.4443237E-544.01
CGTACAG254.4443237E-544.010
ACATCGG351.4469879E-744.032
Produced by FastQC (version 0.11.5)