##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545805_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2456353
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.724946699436114	31.0	31.0	33.0	30.0	34.0
2	32.16099396137282	33.0	31.0	34.0	30.0	34.0
3	32.22471525875963	34.0	31.0	34.0	30.0	34.0
4	35.92107608311998	37.0	35.0	37.0	35.0	37.0
5	35.9089670743578	37.0	35.0	37.0	35.0	37.0
6	35.95048757242953	37.0	35.0	37.0	35.0	37.0
7	36.10220517979297	37.0	35.0	37.0	35.0	37.0
8	36.046118371423	37.0	35.0	37.0	35.0	37.0
9	37.809102763324326	39.0	38.0	39.0	35.0	39.0
10	37.59244579260391	39.0	37.0	39.0	35.0	39.0
11	37.34730309527987	39.0	37.0	39.0	35.0	39.0
12	36.594519191663416	39.0	35.0	39.0	33.0	39.0
13	36.37224820699631	38.0	35.0	39.0	33.0	39.0
14	37.42263143774531	39.0	35.0	41.0	33.0	41.0
15	37.59082224745385	40.0	35.0	41.0	33.0	41.0
16	37.66760966359477	40.0	35.0	41.0	33.0	41.0
17	37.60458167046837	39.0	35.0	41.0	33.0	41.0
18	37.54188547004441	39.0	35.0	41.0	33.0	41.0
19	37.44069683795448	39.0	35.0	41.0	33.0	41.0
20	37.283964071939174	39.0	35.0	41.0	33.0	41.0
21	37.16676634017993	38.0	35.0	41.0	33.0	41.0
22	37.17079955527564	39.0	35.0	41.0	33.0	41.0
23	37.15292468142812	38.0	35.0	41.0	33.0	41.0
24	37.118083190811745	38.0	35.0	41.0	33.0	41.0
25	37.022046098423154	38.0	35.0	41.0	33.0	41.0
26	36.938006467311496	38.0	35.0	41.0	33.0	41.0
27	36.837566099009386	38.0	35.0	41.0	33.0	41.0
28	36.81391640370908	38.0	35.0	41.0	32.0	41.0
29	36.78858657530086	38.0	35.0	41.0	32.0	41.0
30	36.688165748164046	38.0	35.0	41.0	32.0	41.0
31	36.53026254776899	38.0	35.0	41.0	32.0	41.0
32	36.345618483988254	38.0	35.0	41.0	31.0	41.0
33	36.19636102791415	38.0	35.0	41.0	31.0	41.0
34	35.99048426671574	38.0	35.0	41.0	30.0	41.0
35	35.78060848746088	38.0	35.0	41.0	30.0	41.0
36	35.61407379151124	38.0	35.0	40.0	29.0	41.0
37	35.53828663876894	38.0	35.0	40.0	29.0	41.0
38	35.4916394345601	38.0	35.0	40.0	29.0	41.0
39	35.450733668979986	38.0	35.0	40.0	29.0	41.0
40	35.283348118124714	37.0	35.0	40.0	27.0	41.0
41	35.20239517691472	37.0	34.0	40.0	27.0	41.0
42	35.17239582421582	37.0	35.0	40.0	27.0	41.0
43	35.08608249710038	37.0	34.0	40.0	27.0	41.0
44	34.97848761965402	37.0	34.0	40.0	27.0	41.0
45	34.88842890252337	37.0	34.0	40.0	26.0	41.0
46	34.83438862411062	36.0	34.0	40.0	26.0	41.0
47	34.80263300918068	36.0	34.0	40.0	26.0	41.0
48	34.74553738815227	36.0	34.0	40.0	26.0	41.0
49	34.67192419004923	36.0	34.0	40.0	26.0	41.0
50	34.50625378355635	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	7.0
12	9.0
13	13.0
14	25.0
15	59.0
16	135.0
17	312.0
18	675.0
19	1506.0
20	2665.0
21	4329.0
22	6484.0
23	9921.0
24	14566.0
25	22129.0
26	32373.0
27	40479.0
28	42293.0
29	44003.0
30	48444.0
31	58001.0
32	73020.0
33	98973.0
34	197623.0
35	409928.0
36	139789.0
37	182030.0
38	307301.0
39	719252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.68202900804567	23.459046806383284	29.465512489450823	14.393411696120223
2	31.882103264473795	25.69826079557783	29.389016969466525	13.03061897048185
3	28.454012920781334	24.54117954544807	32.79361720404192	14.211190329728668
4	26.634771142421304	27.757614642520846	31.17878415683739	14.42883005822046
5	23.11756494282377	32.15356261905353	30.777620317600928	13.951252120521765
6	21.168374415240805	40.08304181035869	28.487599298635008	10.260984475765495
7	85.52581815398682	5.054525957791897	7.550543427593673	1.8691124606276053
8	86.59602264006843	4.24206129982132	7.492204907030871	1.6697111530793822
9	82.01630628822485	6.30878379451162	8.980427487417321	2.694482429846199
10	49.082766198506484	25.977984434647627	14.851611311566376	10.087638055279514
11	43.227866678771335	19.94851717159545	23.80602462268249	13.017591526950728
12	40.74593513228758	21.517347058830712	25.767916907708297	11.968800901173406
13	21.58419412844978	39.329037805234016	24.545413464595683	14.541354601720519
14	14.991778461809032	39.11465493762501	29.12496697339511	16.76859962717085
15	14.737295494580788	23.58325533830032	46.42553411500708	15.253915052111811
16	16.013944249869624	19.263151509575373	44.307393929129894	20.41551031142511
17	16.094632978240504	20.943732435851036	30.524440094725797	32.43719449118266
18	20.847980725897294	24.33135628307495	34.867382660391236	19.953280330636517
19	26.408744997156354	25.06301008039154	27.11723437144417	21.411010551007937
20	29.061865293791243	21.5475544435185	28.543576595057797	20.84700366763246
21	20.85066763612559	27.958359405183213	29.576367891748458	21.61460506694274
22	22.65472430061966	25.114997722232918	26.4330493214941	25.79722865565332
23	19.028250418404845	29.257602632846336	27.64004196465247	24.07410498409634
24	18.841143760689118	23.78086537236301	40.23660280098178	17.14138806596609
25	16.588820906441377	27.807444614027382	35.36840999644595	20.23532448308529
26	16.923544783669122	32.34868929669311	28.854688230885383	21.87307768875239
27	17.56929887520238	32.5673875049718	30.181777619096277	19.681536000729537
28	14.82519002765482	27.99052090640067	37.78076685232131	19.403522213623205
29	15.571621831227025	23.619162229532968	37.52754591868514	23.281670020554863
30	17.937934816372078	30.896780715149653	32.11049063387876	19.054793834599508
31	27.095169138963332	28.53926125438811	25.550440022260645	18.81512958438791
32	27.3579978122037	26.59157702496343	27.709331679933623	18.34109348289924
33	26.25009516140392	28.845039780520143	25.928358016946262	18.976507041129675
34	18.494369498195088	27.586018784759357	30.454946825639475	23.46466489140608
35	21.409707806654826	25.425742961211196	31.53777164764185	21.62677758449213
36	27.0732260387656	27.839158296873457	26.326794235193397	18.76082142916755
37	19.551261565418326	33.32721314892444	29.022457277109602	18.09906800854763
38	19.95584510858171	31.830522730242762	27.234807049312536	20.978825111862996
39	20.718927613417126	31.85193659054704	27.825968010298197	19.603167785737636
40	23.92380085435603	28.15975554002214	25.858091243400278	22.05835236222155
41	18.501453170615136	26.04878044808706	28.30545935376552	27.14430702753228
42	21.015912615165654	26.62394207998606	25.157866153602516	27.20227915124577
43	21.74923555368467	27.018307222129717	26.467246360763298	24.76521086342232
44	18.838701115027035	30.763534394282903	27.87270396396609	22.52506052672397
45	17.115984551080402	37.0000158771968	24.12051525167596	21.76348432004683
46	20.290243299721173	34.35475275744162	26.449781444279385	18.905222498557823
47	21.070790721040503	27.519823087316848	29.50052374394071	21.90886244770194
48	21.583298491707016	24.87142523896199	32.3513762069214	21.193900062409597
49	19.86090761384866	26.24744896193666	32.447779289051695	21.443864135162983
50	19.116470637567158	32.53152132450018	29.23313546546445	19.118872572468206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1167.0
1	3287.5
2	5408.0
3	17278.5
4	29149.0
5	21250.5
6	13352.0
7	12946.5
8	12541.0
9	12533.5
10	12526.0
11	12183.5
12	11841.0
13	11310.0
14	10779.0
15	10339.0
16	9899.0
17	8608.5
18	7318.0
19	7383.5
20	7449.0
21	7867.5
22	8286.0
23	9288.5
24	10291.0
25	12008.5
26	13726.0
27	21695.5
28	29665.0
29	39701.0
30	49737.0
31	56771.0
32	63805.0
33	66849.5
34	69894.0
35	77019.0
36	84144.0
37	87594.5
38	91045.0
39	106817.5
40	122590.0
41	157860.0
42	193130.0
43	213221.0
44	233312.0
45	244709.5
46	256107.0
47	231526.5
48	206946.0
49	200207.5
50	193469.0
51	183124.0
52	172779.0
53	155077.0
54	137375.0
55	124754.0
56	112133.0
57	97670.0
58	83207.0
59	74163.0
60	65119.0
61	57634.0
62	50149.0
63	42742.0
64	35335.0
65	28264.5
66	21194.0
67	15949.5
68	10705.0
69	9354.5
70	8004.0
71	6668.0
72	5332.0
73	4136.5
74	2941.0
75	2622.5
76	2304.0
77	1726.5
78	1149.0
79	819.5
80	490.0
81	355.5
82	221.0
83	183.5
84	146.0
85	128.5
86	111.0
87	72.5
88	34.0
89	28.0
90	22.0
91	19.5
92	17.0
93	10.0
94	3.0
95	3.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2456353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.846707285017997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.50175083893357	11.146841797981653
2	7.47989423323271	2.07143811940935
3	2.48176421843068	1.030927880491233
4	1.2593259776423298	0.6975007275532985
5	0.7163442226237027	0.49595043829920893
6	0.518912971534494	0.431114161394221
7	0.41449852934784753	0.4017607864165061
8	0.3156002690092549	0.34960196352352724
9	0.2633574933383831	0.32819707094154016
>10	3.1477504105257035	10.28971373779454
>50	0.9970578614630283	9.851713392702646
>100	1.86123278571249	57.369437539955385
>500	0.03805107755485844	3.244811847269075
>1k	0.0032700144773706473	0.8118512738165897
>5k	8.918221301919947E-4	0.8766413110820825
>10k+	2.9727404339733156E-4	0.6024979513691338
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14637	0.5958834092656877	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	8278	0.33700367984568996	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7751	0.3155491087803748	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	5268	0.21446428913108173	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3800	0.15470089193206352	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3479	0.1416327376399076	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAAC	2601	0.10588868945139399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01933761149150794	0.0
2	0.0	0.0	0.0	0.07336079138462591	0.0
3	0.0	0.0	0.0	0.10434168053207336	0.0
4	0.0	0.0	0.0	0.15518942106448055	0.0
5	0.0	0.0	0.0	0.2725178343666403	0.0
6	0.0	0.0	0.0	0.3818262277449536	0.0
7	0.0	0.0	0.0	0.46060155034720174	0.0
8	0.0	0.0	0.0	0.6873197785497442	0.0
9	0.0	0.0	0.0	0.8003735619432549	0.0
10	0.0	0.0	0.0	0.9819435561582558	0.0
11	0.0	0.0	0.0	1.1212150696581478	0.0
12	0.0	0.0	0.0	1.2532400676938535	0.0
13	0.0	0.0	0.0	1.3047391804028168	0.0
14	0.0	0.0	0.0	1.3216341462322394	0.0
15	0.0	0.0	0.0	1.3486660915593158	0.0
16	0.0	0.0	0.0	1.4144546813914776	0.0
17	0.0	0.0	0.0	1.4937999546482121	0.0
18	0.0	0.0	0.0	1.6200847353780177	0.0
19	0.0	0.0	0.0	1.6734565430945796	0.0
20	0.0	0.0	0.0	1.7338306017091192	0.0
21	0.0	0.0	0.0	1.8104889647375602	0.0
22	0.0	0.0	0.0	1.8903634778877465	0.0
23	0.0	0.0	0.0	1.977688060307293	0.0
24	0.0	0.0	0.0	2.0462042711287833	0.0
25	0.0	0.0	0.0	2.1018151707022565	0.0
26	0.0	0.0	0.0	2.1574667810367645	0.0
27	0.0	0.0	0.0	2.2179222611733738	0.0
28	8.142152206950711E-5	0.0	0.0	2.2825302389355278	0.0
29	1.2213228310426066E-4	0.0	0.0	2.3514128466063307	0.0
30	1.2213228310426066E-4	0.0	0.0	2.4414243392541706	0.0
31	1.2213228310426066E-4	0.0	0.0	2.521298852404357	0.0
32	1.2213228310426066E-4	0.0	0.0	2.5992599597859103	0.0
33	1.2213228310426066E-4	0.0	0.0	2.674330603133996	0.0
34	1.2213228310426066E-4	0.0	0.0	2.754408670089356	0.0
35	1.2213228310426066E-4	0.0	0.0	2.863432088140426	0.0
36	1.2213228310426066E-4	0.0	0.0	2.953972820681718	0.0
37	1.2213228310426066E-4	0.0	0.0	3.0395875511378048	0.0
38	1.2213228310426066E-4	0.0	0.0	3.129802597590819	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	20	7.8582315E-4	44.000004	8
ATAGCCG	20	7.8582315E-4	44.000004	15
ACGATTC	20	7.8582315E-4	44.000004	15
CCCGTAG	20	7.8582315E-4	44.000004	42
TACGAAC	20	7.8582315E-4	44.000004	34
TCTAACG	40	8.3200575E-9	44.000004	19
CGTAATA	20	7.8582315E-4	44.000004	27
CGCGTAC	20	7.8582315E-4	44.000004	26
GTGCGTA	55	1.8189894E-12	44.000004	16
TGTAACG	55	1.8189894E-12	44.000004	1
TACCGGT	40	8.3200575E-9	44.000004	17
CTATCGA	30	2.5288973E-6	44.0	11
GTAATCG	25	4.4443237E-5	44.0	41
CTACGTC	30	2.5288973E-6	44.0	16
CGTAGCA	30	2.5288973E-6	44.0	15
ACGTGTA	35	1.4469879E-7	44.0	22
GTCGTAC	35	1.4469879E-7	44.0	39
TATTACG	25	4.4443237E-5	44.0	1
CGTACAG	25	4.4443237E-5	44.0	10
ACATCGG	35	1.4469879E-7	44.0	32
>>END_MODULE