FastQCFastQC Report
Thu 26 May 2016
SRR1545804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545804_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1193515
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA61980.5193064184362995No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59790.5009572565070401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG207.856603E-444.0000042
ACCGGAC408.312782E-944.00000439
CATATTG207.856603E-444.0000041
GTCGACC207.856603E-444.00000419
ATAGGCG207.856603E-444.0000042
TGCCAAC207.856603E-444.00000428
TTGTCGA207.856603E-444.00000411
TTCACGA207.856603E-444.00000441
CCTACGG408.312782E-944.00000438
GTTAGCG551.8189894E-1244.0000041
GCGCATT207.856603E-444.0000049
TATGCGC207.856603E-444.0000041
ACGGGCC800.044.0000045
CTGCGAT207.856603E-444.00000428
TGCATCA408.312782E-944.00000424
AATGCGC207.856603E-444.00000433
ATATCCG207.856603E-444.0000041
ACGCTAG207.856603E-444.0000041
CCCTTCG207.856603E-444.00000439
ACCACGT207.856603E-444.00000441
Produced by FastQC (version 0.11.5)