##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545804_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1193515
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.736439005793812	31.0	31.0	33.0	30.0	34.0
2	32.15211874169994	33.0	31.0	34.0	30.0	34.0
3	32.18744632451205	33.0	31.0	34.0	30.0	34.0
4	35.86058658667884	37.0	35.0	37.0	35.0	37.0
5	35.82830462960248	37.0	35.0	37.0	35.0	37.0
6	35.87985236884329	37.0	35.0	37.0	35.0	37.0
7	36.12572359794389	37.0	35.0	37.0	35.0	37.0
8	36.104133588601734	37.0	35.0	37.0	35.0	37.0
9	37.937374058977056	39.0	39.0	39.0	35.0	39.0
10	37.65840312019539	39.0	38.0	39.0	35.0	39.0
11	37.272634193956506	39.0	37.0	39.0	34.0	39.0
12	35.938288165628414	37.0	35.0	39.0	33.0	39.0
13	35.50282903859608	35.0	35.0	39.0	32.0	39.0
14	36.27318299309184	37.0	35.0	41.0	32.0	41.0
15	36.585595488954894	37.0	35.0	41.0	33.0	41.0
16	36.79271898551757	36.0	35.0	41.0	33.0	41.0
17	36.76862796026862	36.0	35.0	41.0	33.0	41.0
18	36.74042219829663	36.0	35.0	40.0	33.0	41.0
19	36.6678123023171	36.0	35.0	40.0	33.0	41.0
20	36.44366178891761	36.0	35.0	40.0	33.0	41.0
21	36.285713208464074	35.0	35.0	40.0	32.0	41.0
22	36.258804455746265	35.0	35.0	40.0	32.0	41.0
23	36.27890893704729	35.0	35.0	40.0	32.0	41.0
24	36.23644528975338	35.0	35.0	40.0	32.0	41.0
25	36.14156168963105	35.0	35.0	40.0	32.0	41.0
26	36.04435051088591	35.0	35.0	40.0	32.0	41.0
27	35.949037087929355	35.0	35.0	40.0	32.0	41.0
28	36.00787505812663	36.0	35.0	40.0	32.0	41.0
29	36.04799772101733	36.0	35.0	40.0	32.0	41.0
30	35.980001089219655	36.0	35.0	40.0	32.0	41.0
31	35.73173525259423	35.0	35.0	40.0	31.0	41.0
32	35.49610352613918	35.0	35.0	40.0	31.0	41.0
33	35.350688512502984	35.0	35.0	40.0	30.0	41.0
34	35.22318697293289	35.0	35.0	40.0	30.0	41.0
35	35.02800132382082	35.0	35.0	40.0	29.0	41.0
36	34.83742391172294	35.0	34.0	40.0	29.0	41.0
37	34.71061695915007	35.0	34.0	40.0	27.0	41.0
38	34.68356158070908	35.0	34.0	40.0	28.0	41.0
39	34.69840596892372	35.0	34.0	40.0	28.0	41.0
40	34.49739383250315	35.0	34.0	40.0	27.0	41.0
41	34.47774682345844	35.0	34.0	40.0	27.0	41.0
42	34.44603796349438	35.0	34.0	40.0	27.0	41.0
43	34.37445528543839	35.0	34.0	40.0	27.0	41.0
44	34.25366585254479	35.0	34.0	40.0	26.0	41.0
45	34.14315278819286	35.0	34.0	40.0	26.0	41.0
46	34.15737715906377	35.0	34.0	40.0	26.0	41.0
47	34.119509180864924	35.0	34.0	39.0	26.0	41.0
48	34.112899293264014	35.0	34.0	39.0	26.0	41.0
49	34.104994909992755	35.0	34.0	39.0	26.0	41.0
50	33.85909603147007	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	5.0
15	17.0
16	61.0
17	161.0
18	381.0
19	854.0
20	1554.0
21	2468.0
22	3831.0
23	5660.0
24	8149.0
25	12072.0
26	17245.0
27	21480.0
28	22460.0
29	23196.0
30	26663.0
31	31845.0
32	40497.0
33	56137.0
34	123655.0
35	298928.0
36	57451.0
37	67147.0
38	114295.0
39	257299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58727372508934	22.87595882749693	30.062127413564138	14.474640033849596
2	30.63195686690155	25.55803655588744	30.94154660812809	12.868459969082918
3	27.580298529972392	25.006304906096698	32.682287193709335	14.731109370221574
4	24.976309472440647	26.660661994193624	33.352157283318604	15.010871250047131
5	22.566117727887793	32.46042152800761	30.8745177061034	14.098943038001199
6	20.237701243805063	40.235020087724074	28.392772608639188	11.134506059831674
7	86.29451661688374	5.118159386350403	7.021277487086463	1.5660465096793925
8	89.46565397167191	2.97172637126471	6.203692454640286	1.3589272024230947
9	86.6733974855783	3.78855732856311	7.5189670846198	2.019078101238778
10	63.75060221279163	18.352680946615667	10.74934123157229	7.147375609020415
11	57.51716568287789	15.108565874748118	17.938693690485668	9.43557475188833
12	51.30232967327599	19.67717205062358	19.79648349622753	9.224014779872897
13	20.178548237768272	50.220315622342405	20.059739508929507	9.541396630959811
14	11.835041872117232	51.35385814170748	26.182075633737323	10.629024352437968
15	10.433635103035991	20.69106797987457	58.62775080329949	10.24754611378994
16	11.515062651076862	14.419676334189349	56.19820446328701	17.867056551446776
17	12.112038809734273	13.747962949774406	30.61251848531439	43.527479755176934
18	20.86442147773593	19.679266703811848	38.563486843483325	20.892824974968892
19	30.55856021918451	21.56671679869964	26.482113756425345	21.392609225690503
20	33.261500693330206	20.232757862280742	24.99013418348324	21.51560726090581
21	19.836784623569876	27.92842988986314	30.02090463881895	22.213880847748037
22	22.211451050049643	24.098398428172246	25.993305488410286	27.696845033367822
23	16.03415122558158	31.881040456131675	24.768771234546698	27.316037083740046
24	17.57933498950579	21.799977377745567	45.234957248128424	15.385730384620219
25	12.351583348345015	24.027096433643482	42.912238220717796	20.70908199729371
26	13.46836864220391	37.05768255949862	29.62643955040364	19.84750924789383
27	14.42939552498293	37.04913637449047	29.64621307650092	18.875255024025673
28	11.370280222703528	28.341327926335236	43.778838137769526	16.509553713191707
29	11.900227479336246	23.054758423647797	43.59367079592632	21.45134330108964
30	15.436923708541578	32.28338144053489	34.089056274952554	18.190638575970976
31	31.87324834627131	26.488649074372756	23.74507232837459	17.893030250981347
32	31.753936900667355	26.109684419550653	27.338407979790784	14.797970699991204
33	28.469855845967583	27.947784485322764	25.317151439236206	18.265208229473444
34	17.823236406748137	27.467941332953504	30.189817471921177	24.519004788377188
35	18.480119646590115	25.848941990674607	33.88486948215984	21.786068880575442
36	33.685877429274036	22.373577206821864	26.941429307549548	16.99911605635455
37	18.443505108859128	33.804099655220085	30.674185075177103	17.078210160743687
38	19.943276791661603	34.711419630251825	23.562250998102243	21.783052579984332
39	19.89426190705605	33.193717716157735	27.939322086442147	18.972698290344066
40	24.415780279259163	25.37404221982966	23.963418976720025	26.24675852419115
41	15.176767782558242	23.931831606640888	27.375273875904366	33.5161267348965
42	21.976347176198036	24.921848489545585	23.761997126135824	29.33980720812055
43	23.362923800706316	25.503910717502503	26.347720807865844	24.78544467392534
44	18.5574542423011	31.60245158209155	29.14869105122265	20.691403124384696
45	14.658466797652311	42.41857035730594	20.880257055839266	22.042705789202483
46	21.890717753861495	34.3714993108591	26.133563465896952	17.604219469382453
47	21.560097694624698	28.16713656719857	26.924588295915846	23.348177442260884
48	23.618722848058045	22.820324838816436	32.51069320452613	21.05025910859939
49	20.295932602439017	23.80120903382027	34.19672144882972	21.706136914911
50	19.221459302983206	36.19946125519998	25.730971123111146	18.848108318705673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	790.0
1	1210.5
2	1631.0
3	5851.0
4	10071.0
5	7531.5
6	4992.0
7	5333.5
8	5675.0
9	6038.5
10	6402.0
11	6353.0
12	6304.0
13	5985.0
14	5666.0
15	5045.0
16	4424.0
17	4289.0
18	4154.0
19	4105.0
20	4056.0
21	3573.0
22	3090.0
23	3179.0
24	3268.0
25	4407.0
26	5546.0
27	6130.0
28	6714.0
29	7877.5
30	9041.0
31	11118.0
32	13195.0
33	17276.5
34	21358.0
35	24329.5
36	27301.0
37	30699.5
38	34098.0
39	46009.0
40	57920.0
41	89638.5
42	121357.0
43	144365.5
44	167374.0
45	165903.5
46	164433.0
47	142901.5
48	121370.0
49	109324.0
50	97278.0
51	91247.0
52	85216.0
53	75631.0
54	66046.0
55	57446.5
56	48847.0
57	40693.5
58	32540.0
59	26188.5
60	19837.0
61	16708.0
62	13579.0
63	10703.5
64	7828.0
65	6505.5
66	5183.0
67	3833.5
68	2484.0
69	2131.0
70	1778.0
71	1526.0
72	1274.0
73	935.5
74	597.0
75	460.5
76	324.0
77	225.0
78	126.0
79	155.5
80	185.0
81	134.0
82	83.0
83	56.5
84	30.0
85	30.5
86	31.0
87	24.5
88	18.0
89	9.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1193515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.496477538364338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.04868395878717	11.604239489841838
2	7.900244347243526	2.290514294548112
3	2.5438988725934415	1.1063271859926358
4	1.265972722489044	0.7340858054297751
5	0.7646091967437665	0.5542070023111406
6	0.5028327627125965	0.4373582310130104
7	0.38926841031654646	0.39501145365840257
8	0.31583075136844185	0.3662746714509879
9	0.2661522997460718	0.34724437515476475
>10	3.003754618883946	10.194989199094548
>50	0.9717475027007051	10.128018414276415
>100	2.003645241445985	58.89499089427896
>500	0.022191349220328605	1.9158713492050012
>1k	0.0	0.0
>5k	0.0011679657484383475	1.0308676337443903
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6198	0.5193064184362995	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5979	0.5009572565070401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034687456797777995	0.0
2	0.0	0.0	0.0	0.13682274625790208	0.0
3	0.0	0.0	0.0	0.18935664821975426	0.0
4	0.0	0.0	0.0	0.27607529021419924	0.0
5	0.0	0.0	0.0	0.4723024008914844	0.0
6	0.0	0.0	0.0	0.6352664189390163	0.0
7	0.0	0.0	0.0	0.7580968818992639	0.0
8	0.0	0.0	0.0	1.058386362969883	0.0
9	0.0	0.0	0.0	1.1827249762256864	0.0
10	0.0	0.0	0.0	1.3772763643523542	0.0
11	0.0	0.0	0.0	1.5638680703635899	0.0
12	0.0	0.0	0.0	1.7307700364050724	0.0
13	8.378612753086472E-5	0.0	0.0	1.8008990251484063	0.0
14	8.378612753086472E-5	0.0	0.0	1.8245267131121101	0.0
15	8.378612753086472E-5	0.0	0.0	1.8651629849645794	0.0
16	8.378612753086472E-5	0.0	0.0	1.9600088813295182	0.0
17	8.378612753086472E-5	0.0	0.0	2.0845988529679143	0.0
18	8.378612753086472E-5	0.0	0.0	2.2818313971755697	0.0
19	8.378612753086472E-5	0.0	0.0	2.354055039107175	0.0
20	8.378612753086472E-5	0.0	0.0	2.430970704180509	0.0
21	8.378612753086472E-5	0.0	0.0	2.5378818029098924	0.0
22	8.378612753086472E-5	0.0	0.0	2.6423631039408804	0.0
23	8.378612753086472E-5	0.0	0.0	2.7749127576947084	0.0
24	8.378612753086472E-5	0.0	0.0	2.8638936251324867	0.0
25	8.378612753086472E-5	0.0	0.0	2.934860475151129	0.0
26	8.378612753086472E-5	0.0	0.0	3.0146248685605124	0.0
27	8.378612753086472E-5	0.0	0.0	3.0875187995123645	0.0
28	8.378612753086472E-5	0.0	0.0	3.173483366359032	0.0
29	8.378612753086472E-5	0.0	0.0	3.261626372521502	0.0
30	8.378612753086472E-5	0.0	0.0	3.3763295811112553	0.0
31	8.378612753086472E-5	0.0	0.0	3.495473454460145	0.0
32	8.378612753086472E-5	0.0	0.0	3.5901517785700223	0.0
33	8.378612753086472E-5	0.0	0.0	3.687595044888418	0.0
34	8.378612753086472E-5	0.0	0.0	3.793500710087431	0.0
35	8.378612753086472E-5	0.0	0.0	3.9495104795499008	0.0
36	8.378612753086472E-5	0.0	0.0	4.0612811736760746	0.0
37	8.378612753086472E-5	0.0	0.0	4.178833110601878	0.0
38	8.378612753086472E-5	0.0	0.0	4.290603804728051	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	20	7.856603E-4	44.000004	2
ACCGGAC	40	8.312782E-9	44.000004	39
CATATTG	20	7.856603E-4	44.000004	1
GTCGACC	20	7.856603E-4	44.000004	19
ATAGGCG	20	7.856603E-4	44.000004	2
TGCCAAC	20	7.856603E-4	44.000004	28
TTGTCGA	20	7.856603E-4	44.000004	11
TTCACGA	20	7.856603E-4	44.000004	41
CCTACGG	40	8.312782E-9	44.000004	38
GTTAGCG	55	1.8189894E-12	44.000004	1
GCGCATT	20	7.856603E-4	44.000004	9
TATGCGC	20	7.856603E-4	44.000004	1
ACGGGCC	80	0.0	44.000004	5
CTGCGAT	20	7.856603E-4	44.000004	28
TGCATCA	40	8.312782E-9	44.000004	24
AATGCGC	20	7.856603E-4	44.000004	33
ATATCCG	20	7.856603E-4	44.000004	1
ACGCTAG	20	7.856603E-4	44.000004	1
CCCTTCG	20	7.856603E-4	44.000004	39
ACCACGT	20	7.856603E-4	44.000004	41
>>END_MODULE