FastQCFastQC Report
Thu 26 May 2016
SRR1545803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545803_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1924338
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113460.5896053603888715No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA112180.5829537222670861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA408.3182385E-944.00000418
TTCACGG207.8578055E-444.0000042
TCGGGTA207.8578055E-444.0000045
CGACCGT207.8578055E-444.0000048
ACTTACG207.8578055E-444.00000440
AACGCGC207.8578055E-444.00000440
TGTTACG408.3182385E-944.0000041
GTCCGGA207.8578055E-444.00000442
CGTACGT207.8578055E-444.00000417
TGCTCGA207.8578055E-444.00000431
GTATACG207.8578055E-444.0000041
ATCGGTC207.8578055E-444.00000426
TCGGTAT207.8578055E-444.00000434
CTAGCGT408.3182385E-944.00000441
CGAACGG254.4439625E-544.02
GCGAATC254.4439625E-544.011
CGATTCA351.4467696E-744.010
CATTACC700.044.010
TCGGATC502.7284841E-1144.039
CTAACCG454.802132E-1044.01
Produced by FastQC (version 0.11.5)