##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545803_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1924338
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.761678042007173	31.0	31.0	33.0	30.0	34.0
2	32.183294722652676	33.0	31.0	34.0	30.0	34.0
3	32.207901626429454	34.0	31.0	34.0	30.0	34.0
4	35.900113701439146	37.0	35.0	37.0	35.0	37.0
5	35.89170561512582	37.0	35.0	37.0	35.0	37.0
6	35.946730252169836	37.0	35.0	37.0	35.0	37.0
7	36.145808064903356	37.0	35.0	37.0	35.0	37.0
8	36.10626199763243	37.0	35.0	37.0	35.0	37.0
9	37.915061699140175	39.0	39.0	39.0	35.0	39.0
10	37.64360367045706	39.0	37.0	39.0	35.0	39.0
11	37.35382037874843	39.0	37.0	39.0	35.0	39.0
12	36.42396709933494	38.0	35.0	39.0	33.0	39.0
13	36.11499435130419	38.0	35.0	39.0	33.0	39.0
14	37.07183925069297	39.0	35.0	41.0	33.0	41.0
15	37.27942128669704	39.0	35.0	41.0	33.0	41.0
16	37.39930407236151	39.0	35.0	41.0	33.0	41.0
17	37.34077693211899	38.0	35.0	41.0	33.0	41.0
18	37.280194539628695	38.0	35.0	41.0	33.0	41.0
19	37.187527866726114	38.0	35.0	41.0	33.0	41.0
20	37.021128304902774	38.0	35.0	41.0	33.0	41.0
21	36.89767234238476	38.0	35.0	41.0	33.0	41.0
22	36.891624028626985	38.0	35.0	41.0	33.0	41.0
23	36.8802564830087	38.0	35.0	41.0	33.0	41.0
24	36.84493264696742	38.0	35.0	41.0	33.0	41.0
25	36.76394011862781	38.0	35.0	41.0	33.0	41.0
26	36.66281962939983	37.0	35.0	41.0	33.0	41.0
27	36.55180586778414	37.0	35.0	41.0	32.0	41.0
28	36.57781741045492	37.0	35.0	41.0	32.0	41.0
29	36.559260899072825	37.0	35.0	41.0	32.0	41.0
30	36.47994531106282	37.0	35.0	40.0	32.0	41.0
31	36.29751426204752	37.0	35.0	40.0	32.0	41.0
32	36.084852037427936	37.0	35.0	40.0	31.0	41.0
33	35.909270097041166	37.0	35.0	40.0	31.0	41.0
34	35.732020050531666	37.0	35.0	40.0	30.0	41.0
35	35.511053151785184	37.0	35.0	40.0	30.0	41.0
36	35.32809257001629	37.0	35.0	40.0	29.0	41.0
37	35.23719065985289	37.0	35.0	40.0	28.0	41.0
38	35.20201128907708	36.0	35.0	40.0	29.0	41.0
39	35.18479653782236	36.0	35.0	40.0	28.0	41.0
40	34.993141017846135	36.0	34.0	40.0	27.0	41.0
41	34.94558336425306	36.0	34.0	40.0	27.0	41.0
42	34.911284815869145	36.0	34.0	40.0	27.0	41.0
43	34.84544866858109	36.0	34.0	40.0	27.0	41.0
44	34.73433928966741	36.0	34.0	40.0	26.0	41.0
45	34.6165242280722	35.0	34.0	40.0	26.0	41.0
46	34.59580957191512	35.0	34.0	40.0	26.0	41.0
47	34.546076105133295	35.0	34.0	40.0	26.0	41.0
48	34.50712972461179	35.0	34.0	40.0	26.0	41.0
49	34.45648997213587	36.0	34.0	40.0	25.0	41.0
50	34.28191565099271	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	6.0
14	17.0
15	19.0
16	79.0
17	190.0
18	547.0
19	1100.0
20	2011.0
21	3326.0
22	5368.0
23	8215.0
24	12214.0
25	18666.0
26	26817.0
27	32920.0
28	34406.0
29	34900.0
30	39095.0
31	46679.0
32	58912.0
33	80809.0
34	169085.0
35	376944.0
36	100947.0
37	129352.0
38	226800.0
39	514905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.912221241798477	23.443750526154968	29.544965593362498	15.099062638684057
2	31.35535441279027	25.344819880914894	30.513610394847472	12.786215311447366
3	28.201074863147742	25.23995264865112	33.08005142547723	13.47892106272391
4	25.518957688306315	27.602999057338163	32.4227344676455	14.455308786710027
5	22.375279186920384	31.583900541380984	31.90385472822342	14.13696554347521
6	20.73040183169485	39.68689492178609	28.95541219889645	10.627291047622611
7	86.04132953774233	4.426041578974172	7.770776235775628	1.7618526475078702
8	87.63294182207075	3.477871351082814	7.517858089379309	1.3713287374671186
9	83.66290121589867	4.847329315328181	9.096322995232645	2.393446473540511
10	52.112726558432044	24.59313280723033	13.933778785223803	9.360361849113826
11	46.4869996850865	18.636850698785764	22.30424177041663	12.571907845711097
12	42.20433208719051	20.244208657730606	25.56219333609792	11.98926591898097
13	20.59903197878959	42.506513928426294	23.98960057952397	12.904853513260145
14	13.62660821539667	43.4581139072242	28.811882320049804	14.103395557329327
15	12.400732095920779	22.2124179847823	52.25064411761343	13.136205801683488
16	15.018619390148716	17.67267496666386	48.55929675555957	18.74940888762785
17	15.164539701445381	17.858245277077103	29.715673649847375	37.261541371630145
18	20.041177797247677	22.636200085431977	37.01335212421103	20.309269993109318
19	27.150168005828494	23.72233983842755	28.118397079930862	21.009095075813082
20	29.35534194096879	21.764315832249846	28.152954418610456	20.727387808170914
21	20.11496940766123	27.622174482861116	30.913903898379598	21.348952211098048
22	22.492306445125546	23.1801273996564	27.53949669964424	26.788069455573815
23	16.428091114970446	30.78871799029069	26.806101630794586	25.977089263944276
24	17.539694170150984	23.36169633401201	42.59033496194535	16.508274533891658
25	15.72332926959817	25.73472019988173	38.13430904550032	20.407641485019784
26	16.952375310366474	33.13435581483087	29.322083750359862	20.591185124442795
27	16.535972370758152	34.56570519316253	29.826880724695975	19.071441711383343
28	13.143532996802017	28.006722311776826	40.75947156892396	18.09027312249719
29	14.540792729759533	23.21317772657402	40.40184208803235	21.844187455634092
30	17.242553023429355	31.06034386890453	33.62179617094294	18.075306936723173
31	27.661200890903782	27.43961819597181	27.11368792800433	17.785492985120076
32	28.20990907002824	25.583395432611113	28.469946547851784	17.736748949508872
33	26.816286951668573	27.83939204027567	26.681903075239383	18.66241793281638
34	18.98242408558164	26.48838197863369	31.06642388187522	23.46277005390945
35	19.47620428427854	26.221952692302498	32.62774003319583	21.674102990223133
36	29.83483151088842	24.81637841169275	27.582836279281498	17.765953798137332
37	20.008854993249628	31.468796022320404	31.103995244078742	17.41835374035123
38	20.3911682874838	31.643089727480305	26.704664149437363	21.261077835598527
39	19.82785768404511	31.761987758907217	28.515676559939052	19.894477997108616
40	23.369231392821842	25.868636383005477	27.068789370682282	23.693342853490396
41	16.978410237702523	24.986826638563496	28.38331935450009	29.651443769233886
42	21.134436881670478	25.884901716850155	25.887707876682786	27.09295352479658
43	22.26136988408481	27.298738579189312	27.688794795924625	22.751096740801252
44	19.378404417519167	30.949344657747236	28.61477557476909	21.05747534996451
45	15.884943289588419	39.01954854084885	23.128005579061476	21.967502590501255
46	21.073272990503746	33.551330379590276	27.482645980072107	17.892750649833864
47	21.36859532992645	28.3306778746769	27.777032932883934	22.52369386251272
48	22.523278135130106	25.123133254137265	31.456220268996404	20.89736834173622
49	20.44251061923633	24.619479530103337	33.74781353379708	21.190196316863254
50	18.808598073727172	34.15429098214555	27.873689549341123	19.163421394786155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1566.0
1	2800.0
2	4034.0
3	12506.0
4	20978.0
5	15594.5
6	10211.0
7	10506.0
8	10801.0
9	11168.0
10	11535.0
11	11296.0
12	11057.0
13	10355.0
14	9653.0
15	9026.0
16	8399.0
17	7741.5
18	7084.0
19	7134.0
20	7184.0
21	7142.5
22	7101.0
23	7696.0
24	8291.0
25	10626.5
26	12962.0
27	14814.5
28	16667.0
29	18728.0
30	20789.0
31	30539.0
32	40289.0
33	44166.0
34	48043.0
35	55366.0
36	62689.0
37	67338.5
38	71988.0
39	88542.0
40	105096.0
41	133104.0
42	161112.0
43	186582.5
44	212053.0
45	218870.5
46	225688.0
47	198428.5
48	171169.0
49	164323.5
50	157478.0
51	147774.5
52	138071.0
53	124253.5
54	110436.0
55	95735.0
56	81034.0
57	67374.0
58	53714.0
59	46196.5
60	38679.0
61	34919.0
62	31159.0
63	25235.0
64	19311.0
65	15013.0
66	10715.0
67	8694.5
68	6674.0
69	5554.0
70	4434.0
71	3515.0
72	2596.0
73	1907.5
74	1219.0
75	1154.5
76	1090.0
77	877.0
78	664.0
79	459.0
80	254.0
81	222.5
82	191.0
83	133.0
84	75.0
85	68.5
86	62.0
87	38.0
88	14.0
89	15.5
90	17.0
91	12.5
92	8.0
93	5.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1924338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.871015280532118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01090667705607	11.8984341580373
2	7.704984230146014	2.2916187644552077
3	2.6121169521230287	1.1653449332867563
4	1.2587720203483062	0.7487687179722379
5	0.8006392247186036	0.5953159072491873
6	0.535702526019466	0.47798642701530797
7	0.4151037651138746	0.4321110103810391
8	0.32138003594240655	0.38233979402859913
9	0.28055049053874737	0.3754863568586244
>10	3.2011818127959786	11.160059602800137
>50	0.9809100696107673	10.48902266899725
>100	1.8593844163974809	57.028055438482575
>500	0.016954873097849356	1.6139867024937915
>1k	7.064530457437232E-4	0.15621937294853008
>5k	0.0	0.0
>10k+	7.064530457437232E-4	1.1852501449934878
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11346	0.5896053603888715	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	11218	0.5829537222670861	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02276107419798393	0.0
2	0.0	0.0	0.0	0.08912155764735717	0.0
3	0.0	0.0	0.0	0.125809499162829	0.0
4	0.0	0.0	0.0	0.18245235504365656	0.0
5	0.0	0.0	0.0	0.318187345466337	0.0
6	0.0	0.0	0.0	0.43422725113779387	0.0
7	0.0	0.0	0.0	0.5250636842384238	0.0
8	0.0	0.0	0.0	0.7509075848421639	0.0
9	0.0	0.0	0.0	0.8740668219408441	0.0
10	0.0	0.0	0.0	1.121372648671907	0.0
11	0.0	0.0	0.0	1.3301717265885722	0.0
12	0.0	0.0	0.0	1.52972087024213	0.0
13	0.0	0.0	0.0	1.5987835816784786	0.0
14	0.0	0.0	0.0	1.629287578377603	0.0
15	0.0	0.0	0.0	1.6695092026452734	0.0
16	0.0	0.0	0.0	1.7616447838165645	0.0
17	0.0	0.0	0.0	1.8766973369543187	0.0
18	0.0	0.0	0.0	2.0539530997153306	0.0
19	0.0	0.0	0.0	2.128680096739762	0.0
20	0.0	0.0	0.0	2.2108901866512016	0.0
21	0.0	0.0	0.0	2.3123796339312532	0.0
22	0.0	0.0	0.0	2.4148564337450074	0.0
23	0.0	0.0	0.0	2.5390549893002166	0.0
24	0.0	0.0	0.0	2.6337369006900033	0.0
25	0.0	0.0	0.0	2.7046184194252776	0.0
26	0.0	0.0	0.0	2.7817358488997255	0.0
27	0.0	0.0	0.0	2.8599965286763553	0.0
28	0.0	0.0	0.0	2.9427782437388856	0.0
29	0.0	0.0	0.0	3.029717232627532	0.0
30	0.0	0.0	0.0	3.14399029692289	0.0
31	0.0	0.0	0.0	3.250884200176892	0.0
32	0.0	0.0	0.0	3.3404214852068606	0.0
33	0.0	0.0	0.0	3.4368182720499205	0.0
34	0.0	0.0	0.0	3.5389313104038895	0.0
35	0.0	0.0	0.0	3.6745104030580906	0.0
36	0.0	0.0	0.0	3.77574002072401	0.0
37	0.0	0.0	0.0	3.8796718663769045	0.0
38	0.0	0.0	0.0	3.9890601339265763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	40	8.3182385E-9	44.000004	18
TTCACGG	20	7.8578055E-4	44.000004	2
TCGGGTA	20	7.8578055E-4	44.000004	5
CGACCGT	20	7.8578055E-4	44.000004	8
ACTTACG	20	7.8578055E-4	44.000004	40
AACGCGC	20	7.8578055E-4	44.000004	40
TGTTACG	40	8.3182385E-9	44.000004	1
GTCCGGA	20	7.8578055E-4	44.000004	42
CGTACGT	20	7.8578055E-4	44.000004	17
TGCTCGA	20	7.8578055E-4	44.000004	31
GTATACG	20	7.8578055E-4	44.000004	1
ATCGGTC	20	7.8578055E-4	44.000004	26
TCGGTAT	20	7.8578055E-4	44.000004	34
CTAGCGT	40	8.3182385E-9	44.000004	41
CGAACGG	25	4.4439625E-5	44.0	2
GCGAATC	25	4.4439625E-5	44.0	11
CGATTCA	35	1.4467696E-7	44.0	10
CATTACC	70	0.0	44.0	10
TCGGATC	50	2.7284841E-11	44.0	39
CTAACCG	45	4.802132E-10	44.0	1
>>END_MODULE