FastQCFastQC Report
Thu 26 May 2016
SRR1545802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545802_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2423114
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142550.5882925854912315No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA69670.2875225845750551No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA34640.14295654269671176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCA207.8582106E-444.00000427
ACGTCCA207.8582106E-444.00000433
TCGCACA254.4443048E-544.029
GTCGATA454.802132E-1044.01
GTCGACC254.4443048E-544.038
CGCATCG302.5288846E-644.021
CCAATCG302.5288846E-644.028
ATTCGGT254.4443048E-544.014
ATCGTCC351.4469697E-744.010
ATCCGTT302.5288846E-644.026
ATGTACG551.8189894E-1244.01
CGCTACT454.802132E-1044.017
CGGTATG700.044.06
CAATCGT254.4443048E-544.013
TGTCGAT254.4443048E-544.015
TAATGCG2450.044.01
TCGCGAC254.4443048E-544.038
CGCCCCA254.4443048E-544.041
AATCGTC551.8189894E-1244.039
GTACGAT254.4443048E-544.029
Produced by FastQC (version 0.11.5)