FastQCFastQC Report
Thu 26 May 2016
SRR1545801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545801_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4268405
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296180.6938891693735716No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA91370.21406122427464125No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA49120.11507811465875427No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA49100.11503125874887692No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA47230.11065023117534536No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA46230.10830743568147821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG207.8588846E-444.0000041
TACCGAT207.8588846E-444.00000410
GTCGAAC351.4473153E-744.09
ATTTACG1600.042.6250041
CGACGGT4900.041.755128
CGTTTTT190150.041.2463841
TTACGCT650.040.61538335
TTACGCG1200.040.3333321
TCACGAC5150.040.1553425
CACGACG5250.039.3904826
TACGAAC404.128051E-738.50000441
ACGATTG1450.037.9310381
TAACGCG1400.037.7142871
TTAGCGG9350.037.647062
TACGGGA12750.037.443144
TAGGGTC18650.037.1581765
AGGGCGC9250.037.1027036
TTTAGCG2550.037.098041
CGACAAT3050.036.78688420
TACGCTA900.036.66666837