##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545801_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4268405
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.653506169166235	31.0	31.0	33.0	30.0	34.0
2	32.0729829995045	33.0	31.0	34.0	30.0	34.0
3	32.13593977141344	33.0	31.0	34.0	30.0	34.0
4	35.843553505349185	37.0	35.0	37.0	35.0	37.0
5	35.83677954645822	37.0	35.0	37.0	35.0	37.0
6	35.893532830178955	37.0	35.0	37.0	35.0	37.0
7	36.070871906484975	37.0	35.0	37.0	35.0	37.0
8	36.00741049642665	37.0	35.0	37.0	35.0	37.0
9	37.73663651879332	39.0	38.0	39.0	35.0	39.0
10	37.44807908340469	39.0	37.0	39.0	35.0	39.0
11	37.28393439704058	39.0	37.0	39.0	34.0	39.0
12	36.762632880431916	39.0	35.0	39.0	33.0	39.0
13	36.58056088866919	39.0	35.0	39.0	33.0	39.0
14	37.65320160575203	40.0	35.0	41.0	33.0	41.0
15	37.78759232078493	40.0	35.0	41.0	33.0	41.0
16	37.83442152279364	40.0	35.0	41.0	33.0	41.0
17	37.76220485169519	40.0	35.0	41.0	33.0	41.0
18	37.67837142914039	39.0	36.0	41.0	33.0	41.0
19	37.59644621351535	39.0	36.0	41.0	33.0	41.0
20	37.43103524618681	39.0	35.0	41.0	33.0	41.0
21	37.32816801592164	39.0	35.0	41.0	33.0	41.0
22	37.32590581259276	39.0	35.0	41.0	33.0	41.0
23	37.30145382174372	39.0	35.0	41.0	33.0	41.0
24	37.274003286942076	39.0	35.0	41.0	33.0	41.0
25	37.17285051441932	39.0	35.0	41.0	33.0	41.0
26	37.08244625334287	39.0	35.0	41.0	33.0	41.0
27	36.977901347224545	39.0	35.0	41.0	32.0	41.0
28	36.9108990360568	39.0	35.0	41.0	32.0	41.0
29	36.833706501608916	39.0	35.0	41.0	32.0	41.0
30	36.70750807385897	38.0	35.0	41.0	31.0	41.0
31	36.5679126043569	38.0	35.0	41.0	31.0	41.0
32	36.38430959573893	38.0	35.0	41.0	31.0	41.0
33	36.22075623095746	38.0	35.0	41.0	30.0	41.0
34	36.02009298555315	38.0	35.0	41.0	30.0	41.0
35	35.80179270711191	38.0	35.0	41.0	29.0	41.0
36	35.64864908554835	38.0	35.0	41.0	27.0	41.0
37	35.58303136651747	38.0	35.0	41.0	27.0	41.0
38	35.479254897321134	38.0	35.0	41.0	27.0	41.0
39	35.41754238409898	38.0	35.0	41.0	27.0	41.0
40	35.282023378756236	38.0	35.0	40.0	26.0	41.0
41	35.171297709565984	38.0	34.0	40.0	25.0	41.0
42	35.15145048326014	38.0	34.0	40.0	25.0	41.0
43	35.085151713579194	38.0	34.0	40.0	25.0	41.0
44	34.948706601177726	38.0	34.0	40.0	24.0	41.0
45	34.82525088411245	38.0	34.0	40.0	24.0	41.0
46	34.78763683389931	37.0	34.0	40.0	24.0	41.0
47	34.71094003497794	37.0	34.0	40.0	24.0	41.0
48	34.635891392686496	37.0	34.0	40.0	23.0	41.0
49	34.563717594745576	37.0	34.0	40.0	24.0	41.0
50	34.41143331056917	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	10.0
12	18.0
13	28.0
14	84.0
15	151.0
16	301.0
17	674.0
18	1478.0
19	2998.0
20	5525.0
21	9028.0
22	13970.0
23	20235.0
24	29394.0
25	43110.0
26	61656.0
27	75602.0
28	79239.0
29	81936.0
30	89848.0
31	103149.0
32	127223.0
33	165481.0
34	303476.0
35	550982.0
36	254968.0
37	344058.0
38	593580.0
39	1310195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.07647470190856	22.02197307893698	27.901897781489808	14.999654437664656
2	33.52955026526302	24.809173450035786	28.460045379948717	13.201230904752478
3	30.063595183681024	23.737578791140955	32.2375688342601	13.96125719091792
4	26.67795113162879	26.558538845306384	31.83931702825763	14.924192994807195
5	22.69205944609286	32.15885090566617	30.911546584731298	14.237543063509673
6	21.041466308843702	40.41476851423424	28.281102660127143	10.262662516794915
7	84.99685479704948	4.377466524380887	8.580043365144592	2.0456353134250382
8	85.34581418586099	3.7410695564268153	8.712973581466613	2.2001426762455765
9	80.09774142800413	5.833724775413767	10.670355788637677	3.39817800794442
10	42.15591069732136	24.56641298096127	21.055687077491474	12.221989244225888
11	37.25602420576305	23.226685377793345	24.57316023198361	14.944130184460002
12	34.296347230405736	22.306130744388124	28.20732803002527	15.19019399518087
13	22.88536350229184	33.04140539616086	27.370575191435677	16.702655910111623
14	17.84950116026947	35.52130128232911	30.592481266421533	16.036716290979886
15	18.149730402808544	23.92734522614419	41.58818106529254	16.334743305754724
16	18.99997774344281	21.32164122195527	40.64208996100417	19.03629107359775
17	18.70422324029702	21.993016126632785	30.801411768564602	28.501348864505594
18	21.55488994132469	24.243177486672423	34.582215136567406	19.619717435435486
19	26.846468411502656	24.65897683092396	28.886363875967717	19.60819088160566
20	27.324070700882412	23.711105202060253	28.881444005430602	20.083380091626733
21	22.300859454526925	27.492423985071706	30.79208744249901	19.414629117902354
22	22.47921647547503	24.114230022689974	29.253386218036947	24.153167283798048
23	20.01820352098735	27.933830084071214	29.301226102021715	22.74674029291972
24	19.562928072664146	25.24202834548268	36.68627976960949	18.508763812243682
25	18.817309978785985	25.870600376487236	34.61412401119387	20.6979656335329
26	18.61322906331522	30.23557980088581	30.15894696028142	20.992244175517552
27	19.069886760979806	30.499847132594027	30.66567019764994	19.76459590877623
28	17.225146161153873	28.076693753287234	35.290676493912834	19.40748359164606
29	18.223013045856707	25.453043935615295	35.08369051202967	21.24025250649833
30	20.02237369696643	28.611108833393267	32.34589969789652	19.02061777174378
31	26.08620784578783	26.534337767854737	27.998186676287744	19.381267710069686
32	25.69646038742809	26.254092570878353	29.311651541969425	18.737795499724136
33	24.749783584266254	27.29588687109119	28.188749661758898	19.765579882883653
34	20.459094204978207	27.147189641095444	30.304645412045016	22.089070741881333
35	21.74756144274032	25.684933833598265	30.745536096035874	21.82196862762554
36	25.841338860768836	27.466582950774352	27.782180931753196	18.909897256703616
37	20.88543612895215	31.487546284853472	28.736424964360225	18.890592621834152
38	21.278088653724282	31.026156140291278	26.709555442841058	20.986199763143375
39	21.348723937864378	29.634020201925544	28.146017071950762	20.871238788259312
40	23.083962276306956	26.410708449643366	28.033469176425385	22.471860097624287
41	18.94215755065417	26.81926855581886	28.391096908564208	25.847476984962768
42	21.45733593696006	27.492939400080356	26.512034354753123	24.53769030820646
43	22.58803932616516	26.924342933718805	27.308116263569175	23.17950147654686
44	20.745290102509017	28.810152738552226	28.85918744823886	21.585369710699897
45	19.054471166630158	32.97201179363252	26.39868990875983	21.574827130977496
46	20.938711298482687	31.140507988346933	28.32331983492663	19.597460878243748
47	21.83398716850908	27.592953339713546	29.241836236252183	21.331223255525188
48	21.716636542221273	26.11043235119442	31.090442448642996	21.082488657941315
49	20.87449527399579	26.764704848766698	31.70277890687505	20.658020970362465
50	20.379907717285498	30.57783879458486	28.561066721644266	20.481186766485372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2699.0
1	6431.5
2	10164.0
3	34861.5
4	59559.0
5	43058.0
6	26557.0
7	25473.0
8	24389.0
9	24428.5
10	24468.0
11	23833.5
12	23199.0
13	21895.5
14	20592.0
15	19674.0
16	18756.0
17	18534.0
18	18312.0
19	17958.5
20	17605.0
21	18074.5
22	18544.0
23	21101.5
24	23659.0
25	29903.0
26	36147.0
27	41641.5
28	47136.0
29	59664.5
30	72193.0
31	82001.0
32	91809.0
33	103023.5
34	114238.0
35	129024.0
36	143810.0
37	151131.5
38	158453.0
39	179046.0
40	199639.0
41	243233.0
42	286827.0
43	322696.0
44	358565.0
45	370226.0
46	381887.0
47	371295.5
48	360704.0
49	358526.0
50	356348.0
51	335481.5
52	314615.0
53	287830.5
54	261046.0
55	240757.0
56	220468.0
57	196632.0
58	172796.0
59	153909.0
60	135022.0
61	114905.5
62	94789.0
63	78478.0
64	62167.0
65	50670.5
66	39174.0
67	31967.5
68	24761.0
69	21226.5
70	17692.0
71	14924.5
72	12157.0
73	9734.0
74	7311.0
75	6096.5
76	4882.0
77	3776.0
78	2670.0
79	1963.5
80	1257.0
81	959.5
82	662.0
83	509.5
84	357.0
85	254.5
86	152.0
87	117.0
88	82.0
89	62.5
90	43.0
91	29.5
92	16.0
93	17.5
94	19.0
95	13.5
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4268405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.598789186478033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40445353695283	11.738076668399426
2	7.432103875411561	2.169994353782796
3	2.5864224461003267	1.1327590811338053
4	1.3157406972143453	0.7683288425080741
5	0.7768497682135349	0.5670532997856861
6	0.5541365619312971	0.4853833708892841
7	0.4086042128287124	0.4175588734655223
8	0.32017671552773525	0.3739353897926684
9	0.2835898517665291	0.37260616152277026
>10	3.1183280715771495	10.787943858462008
>50	1.0175863525729552	10.710326402059959
>100	1.7469708620890096	55.746409522483106
>500	0.031288898512966914	2.7662551233451165
>1k	0.003422223274855756	1.0413633133439042
>5k	1.6296301308836936E-4	0.21737495645671323
>10k+	1.6296301308836936E-4	0.7046307825692166
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29618	0.6938891693735716	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	9137	0.21406122427464125	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4912	0.11507811465875427	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	4910	0.11503125874887692	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4723	0.11065023117534536	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4623	0.10830743568147821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018906359635507877	0.0
2	0.0	0.0	0.0	0.06721480271904845	0.0
3	0.0	0.0	0.0	0.09572662387941162	0.0
4	0.0	0.0	0.0	0.1457687356284139	0.0
5	0.0	0.0	0.0	0.2711317225052449	0.0
6	2.342795493867147E-5	0.0	0.0	0.4036870915482481	0.0
7	2.342795493867147E-5	0.0	0.0	0.5095580199161045	0.0
8	2.342795493867147E-5	0.0	0.0	0.8071867594569868	0.0
9	2.342795493867147E-5	0.0	0.0	0.9420849239938572	0.0
10	2.342795493867147E-5	0.0	0.0	1.180839212773858	0.0
11	2.342795493867147E-5	0.0	0.0	1.3210086671719297	0.0
12	2.342795493867147E-5	0.0	0.0	1.4703384519510214	0.0
13	4.685590987734294E-5	0.0	0.0	1.5257455653809795	0.0
14	4.685590987734294E-5	0.0	0.0	1.5451204841152608	0.0
15	4.685590987734294E-5	0.0	0.0	1.573070034357096	0.0
16	4.685590987734294E-5	0.0	0.0	1.6398162779773708	0.0
17	4.685590987734294E-5	0.0	0.0	1.718042219517595	0.0
18	4.685590987734294E-5	0.0	0.0	1.8374076499301262	0.0
19	7.028386481601442E-5	0.0	0.0	1.8865126434815815	0.0
20	7.028386481601442E-5	0.0	0.0	1.939108402318899	0.0
21	7.028386481601442E-5	0.0	0.0	2.0114539271695167	0.0
22	7.028386481601442E-5	0.0	0.0	2.090148427808514	0.0
23	7.028386481601442E-5	0.0	0.0	2.1787529533865695	0.0
24	7.028386481601442E-5	0.0	0.0	2.2468580183932874	0.0
25	7.028386481601442E-5	0.0	0.0	2.3069741507659183	0.0
26	7.028386481601442E-5	0.0	0.0	2.367558842237323	0.0
27	7.028386481601442E-5	0.0	0.0	2.4259881618543693	0.0
28	7.028386481601442E-5	0.0	0.0	2.4922892743308096	0.0
29	7.028386481601442E-5	0.0	0.0	2.564142812127715	0.0
30	7.028386481601442E-5	0.0	0.0	2.6539421634076428	0.0
31	7.028386481601442E-5	0.0	0.0	2.7417735664727223	0.0
32	7.028386481601442E-5	0.0	0.0	2.8215691809938375	0.0
33	7.028386481601442E-5	0.0	0.0	2.899677982759368	0.0
34	7.028386481601442E-5	0.0	0.0	2.9850025946460095	0.0
35	7.028386481601442E-5	0.0	0.0	3.088718151159508	0.0
36	7.028386481601442E-5	0.0	0.0	3.1812585731672605	0.0
37	7.028386481601442E-5	0.0	0.0	3.2740098467694607	0.0
38	9.371181975468588E-5	0.0	0.0	3.367955946073533	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	20	7.8588846E-4	44.000004	1
TACCGAT	20	7.8588846E-4	44.000004	10
GTCGAAC	35	1.4473153E-7	44.0	9
ATTTACG	160	0.0	42.625004	1
CGACGGT	490	0.0	41.7551	28
CGTTTTT	19015	0.0	41.246384	1
TTACGCT	65	0.0	40.615383	35
TTACGCG	120	0.0	40.333332	1
TCACGAC	515	0.0	40.15534	25
CACGACG	525	0.0	39.39048	26
TACGAAC	40	4.128051E-7	38.500004	41
ACGATTG	145	0.0	37.931038	1
TAACGCG	140	0.0	37.714287	1
TTAGCGG	935	0.0	37.64706	2
TACGGGA	1275	0.0	37.44314	4
TAGGGTC	1865	0.0	37.158176	5
AGGGCGC	925	0.0	37.102703	6
TTTAGCG	255	0.0	37.09804	1
CGACAAT	305	0.0	36.786884	20
TACGCTA	90	0.0	36.666668	37
>>END_MODULE