FastQCFastQC Report
Thu 26 May 2016
SRR1545800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545800_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences124926
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.6363767350271361No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4900.3922322014632663No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA4090.32739381713974675No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA3410.2729615932632118No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA1540.12327297760274082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCG750.044.0000047
TAAGGGT454.7293724E-1044.0000044
TAAGGGC454.7293724E-1044.0000044
ATACATC1300.044.00000423
GTTGAAG207.829527E-444.01
CTATGCT207.829527E-444.036
TAATAGG254.4200176E-544.02
CGGCGCA302.5095687E-644.022
GATACAT1250.044.022
ACACGGG207.829527E-444.03
ATAGGGT254.4200176E-544.04
TCACAGG207.829527E-444.02
ACACCGG302.5095687E-644.018
CATGAGG207.829527E-444.02
TATGGCC302.5095687E-644.010
ATAGCGG302.5095687E-644.02
CACGCCA302.5095687E-644.027
CAATTGG254.4200176E-544.02
ACGGGTA302.5095687E-644.05
ACGGGCG207.829527E-444.05
Produced by FastQC (version 0.11.5)