FastQCFastQC Report
Thu 26 May 2016
SRR1545799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545799_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2717657
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230930.8497393158886497No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA71050.2614384375953257No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA63520.23373074674250652No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA62660.2305662561537383No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA49140.18081752038612672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG254.444449E-544.021
TACGACA302.5289974E-644.023
TACGAAC254.444449E-544.012
CGTACGT351.4470606E-744.027
ATTGCGA207.85838E-444.010
ATTTCGA454.802132E-1044.013
TCGTAAG1800.042.777781
TATAGCG2400.041.251
CGTAAGG3650.040.98632
CGTTTTT132850.040.8039131
TAGTGCG1800.040.3333321
ACAATCG557.8216544E-1140.037
CTATTCG501.3496901E-939.60000242
CAATACG501.3496901E-939.6000021
CGACGGT3800.039.3684228
CGTATGT452.3530447E-839.1111123
ATCGCTA452.3530447E-839.1111140
CCGTTCG452.3530447E-839.1111142
CGTATGG1300.038.923082
CGGTCTA3800.038.78947431