FastQCFastQC Report
Thu 26 May 2016
SRR1545798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545798_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2759230
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA194660.70548667563052No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123490.4475523968643426No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA31050.11253139462821149No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTC207.8584003E-444.0000049
AAATCGT800.044.00000412
GTCGACA207.8584003E-444.00000440
ACGTTGC207.8584003E-444.00000412
CGCGATC408.3200575E-944.00000440
CGACACT207.8584003E-444.00000442
TCGATAT650.044.00000417
CCTCGCG207.8584003E-444.00000416
ATCGTAA800.044.00000414
TCGGAGC408.3200575E-944.00000432
TGCGATA207.8584003E-444.00000429
CTACGCA800.044.00000428
TACGACA207.8584003E-444.0000044
TTAACGC207.8584003E-444.00000415
ACGTCCA408.3200575E-944.00000430
ATTGGAC408.3200575E-944.0000041
GTATAGT207.8584003E-444.00000420
CATCGGA207.8584003E-444.00000426
CGTAACG207.8584003E-444.0000041
CGTAAAT800.044.00000416
Produced by FastQC (version 0.11.5)