##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545798_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2759230
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74194467297036	31.0	31.0	33.0	30.0	34.0
2	32.162032161146406	33.0	31.0	34.0	30.0	34.0
3	32.198506467384014	34.0	31.0	34.0	30.0	34.0
4	35.8717247203024	37.0	35.0	37.0	35.0	37.0
5	35.848620086038494	37.0	35.0	37.0	35.0	37.0
6	35.89972419841767	37.0	35.0	37.0	35.0	37.0
7	36.13142470906738	37.0	35.0	37.0	35.0	37.0
8	36.09554187218898	37.0	35.0	37.0	35.0	37.0
9	37.91441162933137	39.0	39.0	39.0	35.0	39.0
10	37.66492499719124	39.0	37.0	39.0	35.0	39.0
11	37.289296289182126	39.0	37.0	39.0	35.0	39.0
12	35.8770504814749	35.0	35.0	39.0	33.0	39.0
13	35.38731421447288	35.0	35.0	39.0	32.0	39.0
14	36.16631705222109	36.0	35.0	41.0	32.0	41.0
15	36.49638558583373	36.0	35.0	41.0	33.0	41.0
16	36.70000688597906	36.0	35.0	41.0	33.0	41.0
17	36.66487860743758	36.0	35.0	41.0	33.0	41.0
18	36.64743968425974	36.0	35.0	40.0	33.0	41.0
19	36.567686999633956	36.0	35.0	40.0	33.0	41.0
20	36.35921905749068	36.0	35.0	40.0	33.0	41.0
21	36.17929386096846	35.0	35.0	40.0	32.0	41.0
22	36.14251657165224	35.0	35.0	40.0	32.0	41.0
23	36.20460961935033	35.0	35.0	40.0	32.0	41.0
24	36.16689076300272	35.0	35.0	40.0	32.0	41.0
25	36.07751039239208	35.0	35.0	40.0	32.0	41.0
26	35.94315769254466	35.0	35.0	40.0	32.0	41.0
27	35.85081526367863	35.0	35.0	40.0	32.0	41.0
28	35.893583717196464	36.0	35.0	40.0	32.0	41.0
29	35.92077717334184	36.0	35.0	40.0	32.0	41.0
30	35.862688866096704	36.0	35.0	40.0	32.0	41.0
31	35.615171623967555	35.0	35.0	40.0	31.0	41.0
32	35.359818862508746	35.0	35.0	40.0	31.0	41.0
33	35.19738042859783	35.0	35.0	40.0	30.0	41.0
34	35.046350974728455	35.0	35.0	40.0	30.0	41.0
35	34.82950243364997	35.0	34.0	40.0	29.0	41.0
36	34.62379286974989	35.0	34.0	40.0	27.0	41.0
37	34.523795406689544	35.0	34.0	40.0	27.0	41.0
38	34.475149226414615	35.0	34.0	40.0	27.0	41.0
39	34.49558898678254	35.0	34.0	40.0	27.0	41.0
40	34.26080754413369	35.0	34.0	40.0	25.0	41.0
41	34.20671382958289	35.0	34.0	40.0	25.0	41.0
42	34.191400136994744	35.0	34.0	40.0	25.0	41.0
43	34.056778521544054	35.0	34.0	40.0	25.0	41.0
44	33.93218397886367	35.0	34.0	39.0	24.0	41.0
45	33.80948453010441	35.0	34.0	39.0	23.0	41.0
46	33.81082077246188	35.0	34.0	39.0	24.0	41.0
47	33.75521178009807	35.0	33.0	39.0	23.0	41.0
48	33.744874475850146	35.0	34.0	39.0	23.0	41.0
49	33.75643059839158	35.0	34.0	39.0	24.0	41.0
50	33.48954273474846	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	6.0
13	3.0
14	6.0
15	33.0
16	98.0
17	341.0
18	817.0
19	1773.0
20	3169.0
21	5468.0
22	8740.0
23	13287.0
24	19905.0
25	30256.0
26	42060.0
27	50946.0
28	54400.0
29	56629.0
30	63715.0
31	77195.0
32	97453.0
33	134387.0
34	296506.0
35	726398.0
36	128734.0
37	147183.0
38	243356.0
39	556360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.503462922626962	22.983296064481756	30.095243962989677	15.417997049901603
2	29.73119312271902	25.29053395331306	31.118138031262344	13.860134892705572
3	26.395697350347742	25.231314533402433	33.997999441873276	14.374988674376546
4	24.944712836552227	27.359843144645424	32.793243042442995	14.902200976359348
5	21.557354769265338	32.81154524994292	31.56279106852274	14.068308912269003
6	20.543412473769855	38.94561163802945	28.96597963924718	11.544996248953513
7	85.81361466786024	4.8832101709534905	7.308234543695161	1.9949406174911117
8	88.4685220151999	3.1835330871293803	6.656639714703015	1.6913051829677122
9	85.24823954509047	4.187617559971441	8.330186320096548	2.2339565748415318
10	64.1620307114666	16.749817883974877	11.433515872181733	7.654635532376785
11	59.05821551664776	14.779195645161874	17.158482620151275	9.004106218039091
12	52.05484863530767	19.5721632484425	19.396896960383874	8.976091155865948
13	19.325282778166372	51.827683810338385	19.544909268165394	9.302124143329841
14	11.194971060766953	52.2165241752228	26.99687956422625	9.591625199783998
15	8.343378406294509	20.511664486106632	61.29075865368237	9.85419845391649
16	10.023992200722667	13.526309876306069	58.09541067616691	18.35428724680436
17	10.896373263555411	13.788049564552429	30.02203513299	45.29354203890216
18	20.618324677536847	19.448614287319288	38.8713880321684	21.061673002975468
19	30.608974242814117	22.522406613439255	26.091373317918404	20.77724582582822
20	33.94892778057647	20.53268484323525	24.784813154394524	20.73357422179376
21	19.188614214835297	29.111563733360395	28.487005432675055	23.21281661912925
22	19.723328609793313	24.47204473711869	25.694632198113244	30.10999445497476
23	14.839719776894277	32.69615073770581	24.8935753815376	27.57055410386231
24	16.941501795790856	21.240853426499424	46.948242806870034	14.869401970839691
25	11.995049343476259	24.123976616664795	42.74177940947293	21.13919463038601
26	12.352975286583575	38.62900156927839	28.98863088615302	20.02939225798502
27	14.611938838009156	37.84153550084625	30.289319846478907	17.257205814665685
28	10.462447856829622	28.010604407751437	45.64490818090554	15.882039554513398
29	10.115756932187603	24.29199450571355	44.23864628900092	21.35360227309793
30	14.946633662289841	32.90591940505141	34.09197493503623	18.055471997622526
31	32.74968016439369	24.909413133374166	24.459505006831616	17.881401695400527
32	33.10130000036242	26.396603400223977	25.769580643875283	14.73251595553832
33	29.820638366500802	27.06280375322101	24.40463462632691	18.711923253951284
34	16.947590450959144	27.320194402061443	29.671828734828193	26.060386412151214
35	18.233963823240543	24.518470732776898	34.44229005918318	22.80527538479938
36	33.4943081946775	22.79121349072024	26.301649373194696	17.412828941407565
37	18.62552233775365	34.376220902208225	30.25546982310282	16.742786936935307
38	17.828560866618588	36.11496685669553	23.651997115137195	22.404475161548692
39	19.40885681875016	33.149429369787946	28.85290461469323	18.588809196768665
40	24.897851936953426	25.20938812639758	24.14688155753598	25.745878379113012
41	14.015685535457356	23.84484077079475	27.09842963435451	35.041044059393386
42	22.44278295031585	24.028442717714725	23.24750020839147	30.281274123577955
43	22.675601526512832	24.897054613062338	25.884250316211403	26.54309354421342
44	17.613283416025485	32.245119109316725	29.452600906774716	20.68899656788307
45	13.494308194677501	43.304400140618945	21.61429094348786	21.587000721215702
46	21.01923362677269	35.57467119449991	24.723962844706676	18.682132334020725
47	20.546203107388656	27.84599326623732	27.557035839708906	24.05076778666512
48	23.63840636699369	21.713304073962664	33.88517811128467	20.763111447758973
49	19.85869246130261	22.21869869492576	35.34221503825343	22.580393805518206
50	17.458421371179643	36.97999079453326	26.50250250975816	19.059085324528947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3024.0
1	4058.0
2	5092.0
3	13697.0
4	22302.0
5	17401.5
6	12501.0
7	13776.0
8	15051.0
9	15737.0
10	16423.0
11	15917.5
12	15412.0
13	14267.5
14	13123.0
15	12198.5
16	11274.0
17	10750.5
18	10227.0
19	9446.5
20	8666.0
21	9321.0
22	9976.0
23	9889.0
24	9802.0
25	10301.5
26	10801.0
27	10792.5
28	10784.0
29	19143.5
30	27503.0
31	30018.5
32	32534.0
33	41906.0
34	51278.0
35	56415.5
36	61553.0
37	65909.5
38	70266.0
39	103892.0
40	137518.0
41	210654.5
42	283791.0
43	331592.5
44	379394.0
45	391936.0
46	404478.0
47	336597.0
48	268716.0
49	258241.5
50	247767.0
51	220840.0
52	193913.0
53	171799.5
54	149686.0
55	120007.0
56	90328.0
57	80114.5
58	69901.0
59	56705.0
60	43509.0
61	36223.5
62	28938.0
63	24023.0
64	19108.0
65	14584.0
66	10060.0
67	7893.0
68	5726.0
69	4593.0
70	3460.0
71	2781.0
72	2102.0
73	1957.5
74	1813.0
75	1240.0
76	667.0
77	484.5
78	302.0
79	247.0
80	192.0
81	173.5
82	155.0
83	102.5
84	50.0
85	44.0
86	38.0
87	26.5
88	15.0
89	9.5
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2759230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.917418053186585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70644100948243	10.166840019979535
2	9.559257219599917	2.4696184356702835
3	3.55590623990846	1.3779938237649711
4	1.6870615259516262	0.8716991604865618
5	0.9750736958491744	0.6297717280974746
6	0.6474905561279445	0.5018343719396959
7	0.452641448179386	0.40928711700330456
8	0.3340391347661394	0.3451938519919165
9	0.2548614532844094	0.29629367439456594
>10	1.8761389722645436	5.063911956455837
>50	0.4530026089708574	4.2481094160487105
>100	1.112069245801935	38.4953633129914
>500	0.3712574204967504	31.531534043492048
>1k	0.01419179741960055	2.4260751455021516
>5k	0.0	0.0
>10k+	5.67671896784022E-4	1.1664739421815167
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	19466	0.70548667563052	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12349	0.4475523968643426	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3105	0.11253139462821149	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06570673702445973	0.0
2	0.0	0.0	0.0	0.26790082740474697	0.0
3	0.0	0.0	0.0	0.3736187269636819	0.0
4	3.624199504934347E-5	0.0	0.0	0.5315250993936714	0.0
5	3.624199504934347E-5	0.0	0.0	0.8911181742732573	0.0
6	3.624199504934347E-5	0.0	0.0	1.2309231198559019	0.0
7	3.624199504934347E-5	0.0	0.0	1.4774049281864867	0.0
8	3.624199504934347E-5	0.0	0.0	2.0302765626642216	0.0
9	3.624199504934347E-5	0.0	0.0	2.2732428974750203	0.0
10	3.624199504934347E-5	0.0	0.0	2.6719773270078973	0.0
11	3.624199504934347E-5	0.0	0.0	2.9838034524124484	0.0
12	3.624199504934347E-5	0.0	0.0	3.307371984212987	0.0
13	3.624199504934347E-5	0.0	0.0	3.4201208308114945	0.0
14	3.624199504934347E-5	0.0	0.0	3.4559641639152954	0.0
15	3.624199504934347E-5	0.0	0.0	3.525367584434788	0.0
16	3.624199504934347E-5	0.0	0.0	3.6884565621568335	0.0
17	3.624199504934347E-5	0.0	0.0	3.8926077202697855	0.0
18	7.248399009868695E-5	0.0	0.0	4.264269379500803	0.0
19	7.248399009868695E-5	0.0	0.0	4.367631549381531	0.0
20	7.248399009868695E-5	0.0	0.0	4.483678417529529	0.0
21	7.248399009868695E-5	0.0	0.0	4.63259677518728	0.0
22	7.248399009868695E-5	0.0	0.0	4.776006349597533	0.0
23	7.248399009868695E-5	0.0	0.0	4.9576149867897925	0.0
24	7.248399009868695E-5	0.0	0.0	5.077068602472429	0.0
25	7.248399009868695E-5	0.0	0.0	5.166006458323518	0.0
26	7.248399009868695E-5	0.0	0.0	5.26744780246663	0.0
27	7.248399009868695E-5	0.0	0.0	5.3616045056048245	0.0
28	7.248399009868695E-5	0.0	0.0	5.460436426104384	0.0
29	7.248399009868695E-5	0.0	0.0	5.572786610757349	0.0
30	7.248399009868695E-5	0.0	0.0	5.731200371118029	0.0
31	7.248399009868695E-5	0.0	0.0	5.877545547127278	0.0
32	7.248399009868695E-5	0.0	0.0	5.989424585844602	0.0
33	7.248399009868695E-5	0.0	0.0	6.100832478626283	0.0
34	7.248399009868695E-5	0.0	0.0	6.222351888026732	0.0
35	7.248399009868695E-5	0.0	0.0	6.40852701659521	0.0
36	7.248399009868695E-5	0.0	0.0	6.542767366257978	0.0
37	7.248399009868695E-5	0.0	0.0	6.665663971470302	0.0
38	7.248399009868695E-5	0.0	0.0	6.783994085306408	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACTC	20	7.8584003E-4	44.000004	9
AAATCGT	80	0.0	44.000004	12
GTCGACA	20	7.8584003E-4	44.000004	40
ACGTTGC	20	7.8584003E-4	44.000004	12
CGCGATC	40	8.3200575E-9	44.000004	40
CGACACT	20	7.8584003E-4	44.000004	42
TCGATAT	65	0.0	44.000004	17
CCTCGCG	20	7.8584003E-4	44.000004	16
ATCGTAA	80	0.0	44.000004	14
TCGGAGC	40	8.3200575E-9	44.000004	32
TGCGATA	20	7.8584003E-4	44.000004	29
CTACGCA	80	0.0	44.000004	28
TACGACA	20	7.8584003E-4	44.000004	4
TTAACGC	20	7.8584003E-4	44.000004	15
ACGTCCA	40	8.3200575E-9	44.000004	30
ATTGGAC	40	8.3200575E-9	44.000004	1
GTATAGT	20	7.8584003E-4	44.000004	20
CATCGGA	20	7.8584003E-4	44.000004	26
CGTAACG	20	7.8584003E-4	44.000004	1
CGTAAAT	80	0.0	44.000004	16
>>END_MODULE