FastQCFastQC Report
Thu 26 May 2016
SRR1545797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545797_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1989603
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200541.0079397749199213No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA61500.3091068921789925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA254.4440174E-544.023
CTATACG254.4440174E-544.01
GTTAGCG408.3182385E-944.01
GTTACGC254.4440174E-544.038
CGTTTTT108700.041.712971
CACGACG2200.040.026
CGTAAGG2650.039.849062
ATCGACG501.3496901E-939.6000021
GTTACGG2300.039.2173922
CGTGCGG2750.039.22
ATTAACG1300.038.923081
TATAGCG404.1265594E-738.51
TAGGGAC13300.038.375945
TCGATAG1150.038.260871
CGGTCTA2300.038.2608731
AGGGCGA14450.038.2145356
CGACGGT2250.038.13333528
CGTTAGG3000.038.133332
ATACCGG750.038.133332
GCGTAAG1850.038.0540541
Produced by FastQC (version 0.11.5)