##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545797_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1989603
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.659752221925682	31.0	31.0	33.0	30.0	34.0
2	32.07602370925255	33.0	31.0	34.0	30.0	34.0
3	32.07401074485714	33.0	31.0	34.0	30.0	34.0
4	35.80575371066489	37.0	35.0	37.0	35.0	37.0
5	35.8175520443023	37.0	35.0	37.0	35.0	37.0
6	35.87279824165927	37.0	35.0	37.0	35.0	37.0
7	36.054858682862864	37.0	35.0	37.0	35.0	37.0
8	35.9845913983845	37.0	35.0	37.0	35.0	37.0
9	37.73660524235237	39.0	38.0	39.0	35.0	39.0
10	37.510762197282574	39.0	37.0	39.0	35.0	39.0
11	37.288563597863494	39.0	37.0	39.0	34.0	39.0
12	36.624303441440325	39.0	35.0	39.0	33.0	39.0
13	36.39260093596562	39.0	35.0	39.0	33.0	39.0
14	37.39025423664922	40.0	35.0	41.0	33.0	41.0
15	37.57454426837917	40.0	35.0	41.0	33.0	41.0
16	37.64717835668724	40.0	35.0	41.0	33.0	41.0
17	37.567660985633815	39.0	35.0	41.0	33.0	41.0
18	37.444587689101795	39.0	35.0	41.0	33.0	41.0
19	37.340413137696316	39.0	35.0	41.0	33.0	41.0
20	37.143550748566426	39.0	35.0	41.0	33.0	41.0
21	37.025818215995855	38.0	35.0	41.0	32.0	41.0
22	37.051723886624615	39.0	35.0	41.0	33.0	41.0
23	37.02672141125642	38.0	35.0	41.0	33.0	41.0
24	36.966784328330824	38.0	35.0	41.0	33.0	41.0
25	36.86790430050618	38.0	35.0	41.0	32.0	41.0
26	36.756771074430425	38.0	35.0	41.0	32.0	41.0
27	36.65632792069574	38.0	35.0	41.0	32.0	41.0
28	36.60640188017408	38.0	35.0	41.0	32.0	41.0
29	36.52309983448959	38.0	35.0	41.0	31.0	41.0
30	36.39057691408789	38.0	35.0	41.0	31.0	41.0
31	36.18791889638285	38.0	35.0	41.0	31.0	41.0
32	35.97505180681774	38.0	35.0	41.0	30.0	41.0
33	35.746340350311094	38.0	35.0	41.0	29.0	41.0
34	35.51016760630136	38.0	35.0	41.0	28.0	41.0
35	35.24084905380621	38.0	35.0	40.0	26.0	41.0
36	35.061531873444096	38.0	34.0	40.0	25.0	41.0
37	34.96472612878046	38.0	34.0	40.0	24.0	41.0
38	34.83390455281782	37.0	34.0	40.0	24.0	41.0
39	34.76001342981489	37.0	34.0	40.0	23.0	41.0
40	34.56600839463953	37.0	34.0	40.0	23.0	41.0
41	34.44842714853164	37.0	34.0	40.0	23.0	41.0
42	34.41426254383412	37.0	34.0	40.0	23.0	41.0
43	34.35513567279502	37.0	34.0	40.0	23.0	41.0
44	34.205329907524266	36.0	34.0	40.0	23.0	41.0
45	34.121288015749876	36.0	34.0	40.0	23.0	41.0
46	34.10152075564824	36.0	34.0	40.0	23.0	41.0
47	34.01891231567303	36.0	33.0	40.0	23.0	41.0
48	33.9566079263049	36.0	33.0	40.0	22.0	41.0
49	33.87795203364691	36.0	33.0	40.0	22.0	41.0
50	33.71288392709501	35.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	7.0
12	19.0
13	25.0
14	31.0
15	86.0
16	209.0
17	423.0
18	1011.0
19	2025.0
20	3388.0
21	5225.0
22	7817.0
23	11290.0
24	16230.0
25	23522.0
26	33538.0
27	40866.0
28	42562.0
29	42184.0
30	46112.0
31	52117.0
32	62629.0
33	82345.0
34	152869.0
35	283412.0
36	119773.0
37	157442.0
38	262729.0
39	539708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.471198525534994	21.757355613154985	26.87691966688832	16.8945261944217
2	35.5420654271229	23.853904522661054	27.569871979485356	13.034158070730694
3	29.382947251285806	24.282633269049153	32.97331176119055	13.361107718474491
4	25.839979131515182	26.495285742934644	32.565541969930685	15.099193155619488
5	23.015445794965125	30.931899479443892	31.681445997015484	14.3712087285755
6	20.16005203048045	39.65901740196411	29.53865670689077	10.642273860664666
7	83.3343636896406	4.226370788544247	10.403884594062232	2.035380927752924
8	83.85904122581238	3.753663419285154	10.345179415189865	2.0421159397125956
9	79.4425320026156	4.809954548721529	12.16579387948249	3.581719569180384
10	46.378850454085566	24.07002804076994	18.13452231425063	11.416599190893862
11	40.04924600535886	21.871147158503483	24.58354757205332	13.496059264084343
12	34.95581781893172	22.118281888396833	28.438085386883717	14.487814905787738
13	22.516753342249686	32.96501864944916	28.901142589752833	15.617085418548324
14	17.06410776421226	35.013618294705026	32.603137409824974	15.319136531257744
15	16.68006129866109	21.858481315116634	45.65332882992235	15.808128556299925
16	19.253489263938583	19.340189977598545	42.35925458495991	19.047066173502955
17	19.78374580255458	19.06068698127214	31.616458157732975	29.5391090584403
18	22.778262799161443	21.71342725156727	35.83900908874785	19.669300860523432
19	27.89380595023228	22.856167788247202	29.403504116147793	19.84652214537272
20	27.89812842059446	23.886272789094107	28.384808426605712	19.830790363705724
21	23.24996494275491	25.16089893310374	31.79217160408383	19.796964520057518
22	22.974482849091	22.582997713614223	30.558206838248637	23.884312599046144
23	19.35180033403649	26.984076722843703	29.879377946253598	23.78474499686621
24	19.73288138387407	23.433720194430748	38.41680978567081	18.416588636024372
25	19.599085847779683	24.027708040247227	35.504017635679084	20.869188476294013
26	18.3965343839952	28.309416501684005	31.477184141760944	21.816864972559852
27	18.76349201323078	28.45582761988196	32.25633455518513	20.524345811702133
28	17.096224724228904	25.8548564713664	37.40193395365809	19.646984850746605
29	18.136180936598908	23.743178915592708	37.08181984044053	21.038820307367853
30	20.63592586058626	26.348623318320286	34.32870778743297	18.686743033660484
31	26.39697467283674	24.60209398558406	29.529157324350635	19.47177401722856
32	27.822786756956035	24.229808660320675	29.453614615579088	18.4937899671442
33	26.50001030356307	24.6073211590453	28.378425243629007	20.514243293762625
34	21.009920069481197	25.62415718110598	31.297399531464315	22.068523217948506
35	21.0930522320282	25.51654777360107	32.05162034838106	21.33877964598968
36	27.47588337974963	25.59575955605214	28.261668282566927	18.66668878163131
37	21.327269812118296	30.291620991725487	29.399382690918742	18.98172650523748
38	22.39401528847715	30.427829069417367	26.363450396888222	20.81470524521726
39	22.0072547136288	29.462259556303444	27.13737363685117	21.393112093216587
40	24.332542723347323	25.406023211665847	27.82736053373462	22.434073531252213
41	20.245697257191512	24.34525882801745	28.72181033100573	26.687233583785307
42	22.942014060091385	25.15451575012704	27.121340287484486	24.782129902297093
43	22.682766360927282	25.601439081062903	27.897525285195083	23.818269272814728
44	20.3027438137156	27.8884279929212	29.83560036851573	21.97322782484747
45	18.98232964063685	32.597960497647016	26.45346835524474	21.96624150647139
46	22.126474477571655	29.315496609122523	28.373449376584176	20.184579536721646
47	21.979309440124485	27.27885914928757	28.771217172471093	21.970614238116852
48	23.40728275942487	25.244734753616676	30.55262783580443	20.795354651154025
49	21.99755428595554	24.822841541754812	32.11510034916514	21.064503823124515
50	20.22101896710047	30.684262136717727	28.59228700399024	20.50243189219156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1682.0
1	3960.5
2	6239.0
3	21366.0
4	36493.0
5	25573.5
6	14654.0
7	14029.5
8	13405.0
9	13281.5
10	13158.0
11	12813.0
12	12468.0
13	12073.5
14	11679.0
15	10914.5
16	10150.0
17	9685.5
18	9221.0
19	8547.5
20	7874.0
21	8857.0
22	9840.0
23	10069.0
24	10298.0
25	12129.0
26	13960.0
27	15204.0
28	16448.0
29	19168.0
30	21888.0
31	27022.0
32	32156.0
33	36882.0
34	41608.0
35	49205.0
36	56802.0
37	60163.0
38	63524.0
39	74281.0
40	85038.0
41	108123.0
42	131208.0
43	149240.0
44	167272.0
45	174257.0
46	181242.0
47	174320.0
48	167398.0
49	162561.0
50	157724.0
51	148658.0
52	139592.0
53	131809.0
54	124026.0
55	116282.0
56	108538.0
57	98970.5
58	89403.0
59	79390.0
60	69377.0
61	61619.5
62	53862.0
63	45447.5
64	37033.0
65	31137.5
66	25242.0
67	20434.5
68	15627.0
69	13762.0
70	11897.0
71	10307.5
72	8718.0
73	7029.0
74	5340.0
75	4391.0
76	3442.0
77	2711.5
78	1981.0
79	1505.5
80	1030.0
81	761.5
82	493.0
83	390.0
84	287.0
85	228.0
86	169.0
87	110.0
88	51.0
89	45.0
90	39.0
91	29.0
92	19.0
93	11.0
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1989603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.556722240515214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5934150673019	14.769928955779598
2	6.571235378472323	2.4388117939071554
3	2.327878177121632	1.2959336622780904
4	1.343386657183363	0.997154122358636
5	0.9204924085696373	0.8540660975164803
6	0.6476710588152141	0.7211191164992594
7	0.5214601053635785	0.6773613234319206
8	0.44028077324956333	0.6536134413625126
9	0.36313297639804826	0.6064702001451133
>10	4.619248033995383	19.893691910900106
>50	1.36764196430068	18.425892595211064
>100	1.2789572733588443	36.71970798952265
>500	0.004379053364079927	0.5544531210642021
>1k	2.736908352549954E-4	0.060945657715218704
>5k	2.736908352549954E-4	0.3123464957904959
>10k+	2.736908352549954E-4	1.0185035165174967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20054	1.0079397749199213	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6150	0.3091068921789925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01638517834965066	0.0
2	0.0	0.0	0.0	0.05865491758908687	0.0
3	0.0	0.0	0.0	0.0892640391072993	0.0
4	0.0	0.0	0.0	0.14656190204779546	0.0
5	0.0	0.0	0.0	0.3077498375304018	0.0
6	0.0	0.0	0.0	0.4520499818305461	0.0
7	0.0	0.0	0.0	0.5693598170087198	0.0
8	0.0	0.0	0.0	0.8257928843090807	0.0
9	0.0	0.0	0.0	0.953908895392699	0.0
10	0.0	0.0	0.0	1.1969724613402775	0.0
11	0.0	0.0	0.0	1.340217118691518	0.0
12	5.026128328113699E-5	0.0	0.0	1.4920061942005516	0.0
13	1.0052256656227398E-4	0.0	0.0	1.5483993540419874	0.0
14	1.0052256656227398E-4	0.0	0.0	1.5684033447878798	0.0
15	1.0052256656227398E-4	0.0	0.0	1.5999171694051526	0.0
16	1.0052256656227398E-4	0.0	0.0	1.6546014456150298	0.0
17	1.0052256656227398E-4	0.0	0.0	1.7194887623309776	0.0
18	1.0052256656227398E-4	0.0	0.0	1.8218207350913724	0.0
19	1.0052256656227398E-4	0.0	0.0	1.8628339422487803	0.0
20	1.0052256656227398E-4	0.0	0.0	1.9159601186769422	0.0
21	1.0052256656227398E-4	0.0	0.0	1.9812495256591391	0.0
22	1.0052256656227398E-4	0.0	0.0	2.0470415454741473	0.0
23	1.0052256656227398E-4	0.0	0.0	2.1188146579996108	0.0
24	1.0052256656227398E-4	0.0	0.0	2.176313566073232	0.0
25	1.0052256656227398E-4	0.0	0.0	2.223157082091251	0.0
26	1.0052256656227398E-4	0.0	0.0	2.273267581522545	0.0
27	1.0052256656227398E-4	0.0	0.0	2.333882689159596	0.0
28	1.0052256656227398E-4	0.0	0.0	2.388918794352441	0.0
29	1.0052256656227398E-4	0.0	0.0	2.462149484093058	0.0
30	1.0052256656227398E-4	0.0	0.0	2.5471915754047414	0.0
31	1.0052256656227398E-4	0.0	0.0	2.6191154717800487	0.0
32	1.0052256656227398E-4	0.0	0.0	2.689682313506765	0.0
33	1.0052256656227398E-4	0.0	0.0	2.7615056873155095	0.0
34	1.0052256656227398E-4	0.0	0.0	2.839410676401272	0.0
35	1.0052256656227398E-4	0.0	0.0	2.9294788960410694	0.0
36	1.0052256656227398E-4	0.0	0.0	3.003061414764654	0.0
37	1.0052256656227398E-4	0.0	0.0	3.0877014158100886	0.0
38	1.0052256656227398E-4	0.0	0.0	3.1980249326121846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	25	4.4440174E-5	44.0	23
CTATACG	25	4.4440174E-5	44.0	1
GTTAGCG	40	8.3182385E-9	44.0	1
GTTACGC	25	4.4440174E-5	44.0	38
CGTTTTT	10870	0.0	41.71297	1
CACGACG	220	0.0	40.0	26
CGTAAGG	265	0.0	39.84906	2
ATCGACG	50	1.3496901E-9	39.600002	1
GTTACGG	230	0.0	39.217392	2
CGTGCGG	275	0.0	39.2	2
ATTAACG	130	0.0	38.92308	1
TATAGCG	40	4.1265594E-7	38.5	1
TAGGGAC	1330	0.0	38.37594	5
TCGATAG	115	0.0	38.26087	1
CGGTCTA	230	0.0	38.26087	31
AGGGCGA	1445	0.0	38.214535	6
CGACGGT	225	0.0	38.133335	28
CGTTAGG	300	0.0	38.13333	2
ATACCGG	75	0.0	38.13333	2
GCGTAAG	185	0.0	38.054054	1
>>END_MODULE