FastQCFastQC Report
Thu 26 May 2016
SRR1545796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545796_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1854068
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68020.3668689605774977No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.16126700854553339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA39550.043.3324931
AGGCGAT12300.042.0325167
CGTTATT21150.042.023641
CGGTCTA5350.040.71027831
GTTTTAT46750.040.1882362
CGCATCG1100.040.021
CGTTCGA1050.039.80952514
CACGACG5500.039.60000226
ACGGTCT5500.039.60000230
CGTTTTT19600.039.5102041
TTACGGG5350.039.4766353
TACGGGA4250.039.341184
CTATACG452.3523171E-839.111111
TCACGAC5650.038.93805325
CGACGGT5650.038.5486728
ACGACGG5650.038.5486727
CGTATAG1200.038.52
TTGCGAA1200.038.51
TTACTGG33450.038.27802740
CAAGGAC16100.037.987585
Produced by FastQC (version 0.11.5)