##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545796_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1854068 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48531337577694 31.0 31.0 33.0 30.0 34.0 2 31.675996781132085 31.0 31.0 34.0 30.0 34.0 3 31.751290136068363 31.0 31.0 34.0 30.0 34.0 4 35.660494652839056 37.0 35.0 37.0 33.0 37.0 5 27.089734572841987 35.0 25.0 37.0 0.0 37.0 6 31.324210331012672 35.0 26.0 37.0 17.0 37.0 7 15.044159113905208 0.0 0.0 32.0 0.0 37.0 8 25.125700351874904 17.0 17.0 35.0 17.0 37.0 9 33.225657850736866 32.0 32.0 37.0 28.0 39.0 10 35.93956370532257 37.0 35.0 39.0 32.0 39.0 11 36.933116261108005 38.0 37.0 39.0 34.0 39.0 12 37.06877309785833 39.0 37.0 39.0 34.0 39.0 13 36.69693506387036 39.0 35.0 39.0 33.0 39.0 14 38.01585432681002 40.0 37.0 41.0 33.0 41.0 15 38.25852935275297 40.0 37.0 41.0 33.0 41.0 16 38.28178308454706 40.0 37.0 41.0 33.0 41.0 17 38.29395901336952 40.0 37.0 41.0 34.0 41.0 18 38.276401404910715 40.0 37.0 41.0 34.0 41.0 19 38.2251724316476 40.0 37.0 41.0 34.0 41.0 20 38.27012601479557 40.0 37.0 41.0 34.0 41.0 21 38.15494577329418 40.0 37.0 41.0 34.0 41.0 22 38.08548607710181 40.0 36.0 41.0 34.0 41.0 23 37.99234008677136 40.0 36.0 41.0 34.0 41.0 24 37.85719779425566 40.0 35.0 41.0 33.0 41.0 25 37.68090760425184 40.0 35.0 41.0 33.0 41.0 26 37.436094576897936 39.0 35.0 41.0 33.0 41.0 27 37.24134497763836 39.0 35.0 40.0 33.0 41.0 28 37.27313884927629 39.0 35.0 40.0 33.0 41.0 29 37.160543734102525 39.0 35.0 40.0 32.0 41.0 30 36.96383088430414 39.0 35.0 40.0 32.0 41.0 31 36.81855196249544 39.0 35.0 40.0 31.0 41.0 32 36.64694606670305 39.0 35.0 40.0 31.0 41.0 33 36.464255895684516 39.0 35.0 40.0 31.0 41.0 34 36.22009332991023 38.0 35.0 40.0 30.0 41.0 35 36.039054662504284 38.0 35.0 40.0 30.0 41.0 36 35.86510311380165 38.0 35.0 40.0 29.0 41.0 37 35.783208598605874 38.0 35.0 40.0 29.0 41.0 38 35.57323625670687 38.0 35.0 40.0 29.0 41.0 39 35.50012135477232 38.0 35.0 40.0 28.0 41.0 40 35.24968555630107 38.0 34.0 40.0 27.0 41.0 41 35.02566356789503 38.0 34.0 40.0 26.0 41.0 42 35.036446883285834 38.0 34.0 40.0 27.0 41.0 43 34.92079956074966 38.0 34.0 40.0 26.0 41.0 44 34.68780918499213 37.0 34.0 40.0 26.0 40.0 45 34.49711445319158 37.0 34.0 40.0 25.0 40.0 46 34.41544700625867 37.0 33.0 40.0 25.0 40.0 47 34.22412931996022 36.0 33.0 39.0 24.0 40.0 48 34.1019568861552 36.0 33.0 39.0 24.0 40.0 49 33.94934382126222 36.0 33.0 39.0 24.0 40.0 50 34.056113907364775 36.0 33.0 39.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 5.0 13 8.0 14 17.0 15 34.0 16 105.0 17 239.0 18 670.0 19 1292.0 20 2466.0 21 3889.0 22 6316.0 23 9522.0 24 14313.0 25 20737.0 26 26476.0 27 29388.0 28 31693.0 29 35771.0 30 44436.0 31 58607.0 32 79902.0 33 132946.0 34 147258.0 35 191574.0 36 270615.0 37 481979.0 38 207524.0 39 56285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.05721904482468 23.670275308133252 27.70896213083878 14.56354351620329 2 33.20805925133274 23.797724786793147 30.239074295009676 12.75514166686443 3 30.514145112261254 23.764770224177322 32.883475686975885 12.83760897658554 4 26.70166358515437 26.874203103661788 30.910247089103528 15.51388622208031 5 17.424765434708974 48.11317600001726 23.21721749148359 11.244841073790173 6 19.898515049070475 42.028933135138516 27.94271839004826 10.129833425742746 7 40.20553722948673 55.38103241089324 3.5239268462645383 0.8895035133554972 8 89.37989329409709 2.3095700912803627 6.832597294166126 1.4779393204564233 9 85.17287391832447 3.9167926958450283 8.327472347292549 2.5828610385379607 10 43.345120027960135 25.64868170962446 15.967753070545418 15.038445191869984 11 36.79325677375371 23.75452248784834 22.19659688857151 17.255623849826435 12 33.74115728225718 20.96115137093138 27.50303656608064 17.794654780730802 13 24.198950631799914 28.79791895442886 27.9974089407724 19.005721472998832 14 19.4770094732232 32.491958223754466 29.470871618516686 18.56016068450564 15 18.711935053083273 23.845997018448085 38.327396837656444 19.1146710908122 16 21.021289402546184 19.35899869907684 38.37394313477176 21.24576876360522 17 20.614993624829296 20.223961580697146 29.818323815523485 29.34272097895007 18 22.823380803724564 23.34358826105623 31.942625621066757 21.890405314152446 19 27.53911938504952 25.27086385181126 26.135449185251026 21.05456757788819 20 28.726184800125992 24.087250305814027 25.53396099819424 21.65260389586574 21 24.994121035474425 25.70439703398149 29.142728314171862 20.158753616372216 22 23.97360830347107 22.508505621153056 27.271977079589316 26.24590899578656 23 22.24465337840899 27.489984186124783 26.344556941816588 23.920805493649638 24 21.974922171139355 25.512278945540295 32.62507092512249 19.887727958197864 25 23.20324820880356 24.167775939178064 30.32164947563951 22.30732637637886 26 20.838502147709793 28.021572024327046 27.101594979256426 24.038330848706735 27 20.64778638108203 27.475421613446755 29.46914568397707 22.40764632149414 28 19.551548271152946 26.258853504833695 33.43243074148305 20.757167482530306 29 20.359663183874595 25.473121805672715 32.29358362260715 21.87363138784554 30 22.330842234481153 26.421846447918846 30.697795334367456 20.549515983232546 31 28.421665224792186 24.433084439189933 25.968465018543007 21.176785317474874 32 27.46263891076271 24.920876688449397 26.3727112489941 21.243773151793786 33 26.21689172133924 24.6421921957555 26.336736300933943 22.80417978197132 34 21.564635169799597 26.14995782247469 28.181005227424237 24.104401780301476 35 22.23181674027058 24.275107493360544 29.205455247596095 24.287620518772773 36 27.85728463033718 25.329060207069 25.671280665002577 21.14237449759124 37 22.393784909722836 28.577862300627594 26.56321127380441 22.465141515845158 38 23.003417350388446 29.545518287355154 24.413182256529968 23.037882105726435 39 22.291523288250485 27.331575756660488 25.16493461944222 25.211966335646807 40 23.82248116034579 24.46927512906754 27.494245087019465 24.213998623567203 41 21.369820308640243 23.57529497299991 28.08780476228488 26.96707995607497 42 22.530403415624452 26.312411411016207 25.032037659891653 26.125147513467684 43 22.54636831011592 25.377602115995746 26.47384022592483 25.602189347963506 44 22.068122636278712 26.314299151918913 28.17081142654962 23.446766785252752 45 21.48896372732823 29.087498408904093 25.86523255889212 23.55830530487555 46 23.239708575952985 27.29349732588017 27.530327905988344 21.936466192178496 47 22.971325755042425 26.95106112612914 27.069719125727858 23.007893993100577 48 22.577003648194133 26.449353529644004 28.07119264234106 22.902450179820804 49 22.649438963403714 25.345402649741 29.313056479050392 22.692101907804894 50 21.962139468455312 26.880351745459173 28.280947624358976 22.87656116172654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 770.0 1 1696.0 2 2622.0 3 11893.0 4 21164.0 5 15314.0 6 9464.0 7 9405.0 8 9346.0 9 9471.0 10 9596.0 11 9458.5 12 9321.0 13 8880.5 14 8440.0 15 8001.0 16 7562.0 17 7298.5 18 7035.0 19 6907.0 20 6779.0 21 6897.0 22 7015.0 23 7868.0 24 8721.0 25 10308.0 26 11895.0 27 13966.5 28 16038.0 29 19422.0 30 22806.0 31 27165.5 32 31525.0 33 35540.5 34 39556.0 35 42498.0 36 45440.0 37 52340.0 38 59240.0 39 73988.5 40 88737.0 41 105489.5 42 122242.0 43 128651.0 44 135060.0 45 140930.5 46 146801.0 47 152301.0 48 157801.0 49 154039.5 50 150278.0 51 139748.0 52 129218.0 53 120686.0 54 112154.0 55 107630.5 56 103107.0 57 99298.0 58 95489.0 59 88204.5 60 80920.0 61 71092.5 62 61265.0 63 53053.5 64 44842.0 65 37726.0 66 30610.0 67 26293.0 68 21976.0 69 19146.0 70 16316.0 71 13578.0 72 10840.0 73 8531.0 74 6222.0 75 4554.0 76 2886.0 77 2247.5 78 1609.0 79 1174.0 80 739.0 81 556.0 82 373.0 83 261.5 84 150.0 85 103.0 86 56.0 87 39.5 88 23.0 89 18.0 90 13.0 91 8.5 92 4.0 93 3.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1854068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.607872070672673 #Duplication Level Percentage of deduplicated Percentage of total 1 71.15790238687192 18.933603635273016 2 7.288669480412864 3.878719702004835 3 3.596785301104667 2.8710840947240657 4 2.4307257062509526 2.587057545232833 5 1.8098942509903797 2.4078717345898975 6 1.4414422655966492 2.3012226840153733 7 1.1918664599034887 2.219912120330628 8 1.001913983397048 2.1327039276837376 9 0.8845071182553436 2.1181367023323774 >10 8.67893354366945 48.391661490715265 >50 0.4557485308204029 7.509840316754447 >100 0.055099244814620994 2.567827852781447 >500 0.004257668250238437 0.8580527932700742 >1k 0.0020036085883474997 0.7690221524026605 >5k 2.5045107354343746E-4 0.4532832478893325 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6802 0.3668689605774977 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2990 0.16126700854553339 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009924123602802055 0.0 2 0.0 0.0 0.0 0.030797144441304204 0.0 3 0.0 0.0 0.0 0.04282475076426539 0.0 4 0.0 0.0 0.0 0.05992228979735371 0.0 5 0.0 0.0 0.0 0.10134471874817969 0.0 6 0.0 0.0 0.0 0.19864427841913027 0.0 7 0.0 0.0 0.0 0.26703443455148357 0.0 8 0.0 0.0 0.0 0.6110886979334091 0.0 9 0.0 0.0 0.0 0.7536940392693257 0.0 10 0.0 0.0 0.0 0.9933831984587405 0.0 11 0.0 0.0 0.0 1.193160121419495 0.0 12 0.0 0.0 0.0 1.3588498372227988 0.0 13 0.0 0.0 0.0 1.4264309615397062 0.0 14 0.0 0.0 0.0 1.4570123641635582 0.0 15 0.0 0.0 0.0 1.4957380203962314 0.0 16 0.0 0.0 0.0 1.575292815581737 0.0 17 0.0 0.0 0.0 1.671837278891605 0.0 18 0.0 0.0 0.0 1.8203215847530942 0.0 19 0.0 0.0 0.0 1.887093677254556 0.0 20 5.3935454363054645E-5 0.0 0.0 1.9619560879104758 0.0 21 5.3935454363054645E-5 0.0 0.0 2.0594713893988787 0.0 22 5.3935454363054645E-5 0.0 0.0 2.1638904290457526 0.0 23 5.3935454363054645E-5 0.0 0.0 2.278934753202148 0.0 24 5.3935454363054645E-5 0.0 0.0 2.3704092838018886 0.0 25 5.3935454363054645E-5 0.0 0.0 2.445864984455802 0.0 26 5.3935454363054645E-5 0.0 0.0 2.5222915232882506 0.0 27 5.3935454363054645E-5 0.0 0.0 2.5971539339441705 0.0 28 5.3935454363054645E-5 0.0 0.0 2.676924470947128 0.0 29 5.3935454363054645E-5 0.0 0.0 2.7770826096993204 0.0 30 5.3935454363054645E-5 0.0 0.0 2.895039448391321 0.0 31 5.3935454363054645E-5 0.0 0.0 2.99530545805224 0.0 32 5.3935454363054645E-5 0.0 0.0 3.0938994686279035 0.0 33 5.3935454363054645E-5 0.0 0.0 3.1964847028264334 0.0 34 5.3935454363054645E-5 0.0 0.0 3.3058658042747084 0.0 35 5.3935454363054645E-5 0.0 0.0 3.4390324410970905 0.0 36 5.3935454363054645E-5 0.0 0.0 3.544044770741958 0.0 37 1.0787090872610929E-4 0.0 0.0 3.654666387640583 0.0 38 1.0787090872610929E-4 0.0 0.0 3.7853519935622644 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTA 3955 0.0 43.332493 1 AGGCGAT 1230 0.0 42.032516 7 CGTTATT 2115 0.0 42.02364 1 CGGTCTA 535 0.0 40.710278 31 GTTTTAT 4675 0.0 40.188236 2 CGCATCG 110 0.0 40.0 21 CGTTCGA 105 0.0 39.809525 14 CACGACG 550 0.0 39.600002 26 ACGGTCT 550 0.0 39.600002 30 CGTTTTT 1960 0.0 39.510204 1 TTACGGG 535 0.0 39.476635 3 TACGGGA 425 0.0 39.34118 4 CTATACG 45 2.3523171E-8 39.11111 1 TCACGAC 565 0.0 38.938053 25 CGACGGT 565 0.0 38.54867 28 ACGACGG 565 0.0 38.54867 27 CGTATAG 120 0.0 38.5 2 TTGCGAA 120 0.0 38.5 1 TTACTGG 3345 0.0 38.278027 40 CAAGGAC 1610 0.0 37.98758 5 >>END_MODULE