FastQCFastQC Report
Thu 26 May 2016
SRR1545795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545795_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences713068
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.2510279524533425No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17260.2420526513600386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG253.88931E-545.00000421
GCGTTAA253.88931E-545.0000041
CGACTAG253.88931E-545.0000042
TACGTTC207.0314837E-445.031
TGCGTAG207.0314837E-445.02
TGCGATA302.164339E-644.9999961
CGTTTTA10300.043.4708751
CGTTTTT14650.042.6962431
TCACGAC2500.042.325
AGGCGAT4950.042.272737
AGGTACC2950.041.9491547
CGGTCTA2450.041.3265331
CGACGGT2400.041.24999628
GACGGTC2400.041.24999629
AAGGTAC1700.041.0294156
TTTAGGC1650.040.909094
CGAAGGA1600.040.781254
ACGGTCT2450.040.40816530
GTAAGGC1950.040.3846174
TAGGCGA1400.040.1785746
Produced by FastQC (version 0.11.5)