##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545795_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	713068
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.952691748893514	31.0	31.0	33.0	30.0	33.0
2	31.120769688164383	31.0	31.0	33.0	30.0	34.0
3	31.086227400472325	31.0	31.0	34.0	28.0	34.0
4	35.06412151435768	35.0	35.0	37.0	32.0	37.0
5	32.74843633426265	35.0	35.0	37.0	28.0	37.0
6	33.941361833654014	35.0	35.0	37.0	28.0	37.0
7	15.460038874272861	0.0	0.0	35.0	0.0	37.0
8	24.98744579759574	17.0	17.0	35.0	17.0	37.0
9	32.72134494886883	32.0	32.0	37.0	27.0	39.0
10	35.37257456511861	37.0	34.0	37.0	32.0	39.0
11	36.345822558297385	37.0	35.0	39.0	32.0	39.0
12	36.335043782640646	37.0	35.0	39.0	32.0	39.0
13	36.17549097701762	38.0	35.0	39.0	32.0	39.0
14	37.206284674112425	39.0	36.0	40.0	32.0	41.0
15	37.48780901681186	39.0	36.0	41.0	32.0	41.0
16	37.459381152989614	39.0	36.0	41.0	32.0	41.0
17	37.393920355421926	39.0	36.0	41.0	32.0	41.0
18	37.34408078892896	39.0	36.0	40.0	32.0	41.0
19	37.29206611431168	39.0	36.0	40.0	32.0	41.0
20	37.41741320603364	39.0	36.0	41.0	32.0	41.0
21	37.299657255689496	39.0	35.0	41.0	32.0	41.0
22	37.36825800624905	39.0	35.0	41.0	32.0	41.0
23	37.33539017316721	39.0	35.0	41.0	32.0	41.0
24	37.16805550101814	39.0	35.0	40.0	32.0	41.0
25	36.91895022634588	39.0	35.0	40.0	31.0	41.0
26	36.799904076469566	39.0	35.0	40.0	31.0	41.0
27	36.73303527854286	39.0	35.0	40.0	31.0	41.0
28	36.632624097561525	38.0	35.0	40.0	31.0	41.0
29	36.57375874390661	38.0	35.0	40.0	31.0	41.0
30	36.290617444619585	38.0	35.0	40.0	30.0	41.0
31	36.18750245418389	38.0	35.0	40.0	30.0	41.0
32	36.12570189659331	38.0	35.0	40.0	30.0	41.0
33	35.9561248015617	38.0	35.0	40.0	29.0	41.0
34	35.789596504120226	38.0	35.0	40.0	28.0	41.0
35	35.62138253294216	38.0	34.0	40.0	27.0	41.0
36	35.422604856759804	38.0	34.0	40.0	26.0	41.0
37	35.30685713003528	38.0	34.0	40.0	26.0	41.0
38	35.14894371925258	38.0	34.0	40.0	25.0	41.0
39	35.02715589537043	38.0	34.0	40.0	25.0	41.0
40	34.861216602063195	38.0	34.0	40.0	24.0	41.0
41	34.69115708459782	38.0	33.0	40.0	24.0	41.0
42	34.652644628562776	38.0	33.0	40.0	24.0	41.0
43	34.58386577437215	38.0	33.0	40.0	23.0	41.0
44	34.36036955802252	37.0	33.0	40.0	23.0	41.0
45	34.27240038818177	37.0	33.0	40.0	23.0	41.0
46	34.229230592313776	37.0	33.0	40.0	23.0	41.0
47	34.13086129233117	37.0	33.0	40.0	23.0	41.0
48	34.04139857629286	37.0	33.0	40.0	23.0	41.0
49	33.909824869437415	36.0	33.0	40.0	23.0	41.0
50	33.79910892088833	36.0	33.0	40.0	23.0	41.0
51	33.624258836464406	36.0	32.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	3.0
14	6.0
15	48.0
16	88.0
17	216.0
18	466.0
19	841.0
20	1480.0
21	2375.0
22	3682.0
23	5457.0
24	7615.0
25	10059.0
26	11977.0
27	13336.0
28	14691.0
29	17211.0
30	20722.0
31	26703.0
32	34470.0
33	48995.0
34	62141.0
35	71092.0
36	90021.0
37	132432.0
38	112331.0
39	24605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.67392731128027	24.283097825172355	27.911082813981274	14.1318920495661
2	32.9784817156288	23.56142752163889	30.687676350642572	12.77241441208973
3	31.038554527758922	23.298339008341422	32.6288656902287	13.034240773670955
4	27.212131241340238	27.476061189115203	29.604048982705716	15.707758586838844
5	21.307505034582956	36.52624995091632	28.23798010848895	13.92826490601177
6	19.90707758586839	42.481221987243856	27.630324176656362	9.981376250231394
7	39.439997307409676	56.053279631115124	3.8334352403978302	0.6732878210773727
8	89.75960216977904	2.5251448669692094	6.310197624910948	1.4050553383408035
9	85.43350704280658	4.116998659314399	7.76545855374242	2.684035744136604
10	43.311297099294876	26.127802678005462	15.618566532224134	14.942333690475524
11	37.11932101847229	24.119719297458307	21.71532027800995	17.04563940605945
12	34.02971385618202	21.280859609462212	27.085635591556485	17.603790942799286
13	24.404825346250288	29.421597940168397	27.213954349374813	18.9596223642065
14	19.37164478002098	33.39793680266118	28.92094442605754	18.3094739912603
15	18.728087643815176	24.18535118670309	38.19930217033999	18.887258999141736
16	20.95957187813785	19.642165964536343	38.25217230334274	21.14608985398307
17	20.46649127432447	20.453028322684514	29.402946142583875	29.67753426040714
18	22.783521347192696	23.65721081299399	31.902146779830254	21.65712105998306
19	27.88401667162178	25.414967436485718	25.774961153774957	20.926054738117543
20	29.141540498241405	24.24887948975413	25.101673332697583	21.50790667930688
21	25.022858969972006	26.12822339524421	28.78813801769256	20.060779617091217
22	23.918335979177304	22.81423370562134	26.91987299948953	26.34755731571183
23	22.20251084048085	27.8642429614006	26.032580342968693	23.90066585514986
24	21.784458144244308	25.86681775090174	32.63209118905911	19.716632915794847
25	23.120375616350756	24.402721760056544	30.28575114855806	22.19115147503464
26	20.620754261865628	28.62167423022769	26.727605221381413	24.029966286525266
27	20.54614707152754	28.22087094077984	29.031733298928014	22.201248688764604
28	19.30138500114996	26.901641919143753	33.275227608026164	20.52174547168012
29	20.290491229447962	25.8728480313238	32.137327716290734	21.699333022937502
30	22.142067797180633	27.089001329466473	30.453477087739177	20.315453785613713
31	28.75041370528477	24.68376087554062	25.614247168572984	20.951578250601628
32	27.919076441517497	25.03267570554281	26.182075201804032	20.866172651135656
33	26.643041056392942	24.770709104882002	25.948997851537303	22.637251987187756
34	21.609439772924883	26.35456926969097	27.777154492979633	24.258836464404517
35	22.36196267396658	24.29067073546983	29.130461610954356	24.21690497960924
36	28.242888476274352	25.69839061632271	25.247662214543354	20.81105869285959
37	22.3707977359803	29.231013031015273	26.340264883573518	22.05792434943091
38	22.872292684568652	30.192211682476284	24.083397375846342	22.852098257108718
39	22.304043934098853	27.761026998827603	25.04571793994402	24.889211127129528
40	24.04553282435897	24.507340113425368	27.296414928169543	24.15071213404612
41	21.32713850572456	23.776974986957768	27.844188773020246	27.05169773429743
42	22.39365670595231	26.408982032569124	24.694278806509338	26.503082454969228
43	22.326201708672947	25.500092557792524	26.34236846976726	25.831337263767267
44	21.902960166491837	26.37490393623049	28.304874149449983	23.4172617478277
45	21.498370421895245	29.565763713979592	25.634020878794168	23.30184498533099
46	23.179556507934727	27.78865409750543	27.347181474978544	21.684607919581303
47	22.949283939259647	27.227136822855602	26.69422832044069	23.129350917444057
48	22.312458278873823	26.869807648078442	28.149208771112992	22.66852530193474
49	22.54539539006098	25.72040815181722	29.346710271671146	22.387486186450662
50	21.907447817038488	27.664542512074586	27.969983227406082	22.458026443480847
51	21.74841670079151	28.94240100523372	26.469986032187677	22.839196261787094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	678.5
2	1027.0
3	3902.5
4	6778.0
5	5012.0
6	3246.0
7	3173.0
8	3100.0
9	3191.0
10	3282.0
11	3217.5
12	3153.0
13	3002.5
14	2852.0
15	2804.5
16	2757.0
17	2642.5
18	2528.0
19	2463.5
20	2399.0
21	2561.0
22	2723.0
23	2914.5
24	3106.0
25	3647.5
26	4969.0
27	5749.0
28	6912.0
29	8075.0
30	9687.5
31	11300.0
32	12929.5
33	14559.0
34	15861.0
35	17163.0
36	19085.0
37	21007.0
38	25798.0
39	30589.0
40	37006.0
41	43423.0
42	46521.5
43	49620.0
44	51834.0
45	54048.0
46	55752.5
47	57457.0
48	56941.5
49	56426.0
50	54635.5
51	52845.0
52	49821.5
53	46798.0
54	43883.5
55	40969.0
56	39417.0
57	37865.0
58	35469.5
59	33074.0
60	29735.5
61	26397.0
62	23046.5
63	19696.0
64	17040.5
65	14385.0
66	12037.0
67	9689.0
68	8362.5
69	7036.0
70	6172.0
71	5308.0
72	4608.0
73	3908.0
74	3011.5
75	1534.5
76	954.0
77	762.5
78	571.0
79	436.5
80	302.0
81	226.5
82	151.0
83	108.0
84	65.0
85	51.0
86	37.0
87	23.0
88	9.0
89	8.0
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	713068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.48096826698185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.92549932735183	23.745815178856848
2	8.827672793334477	6.264287565031992
3	5.1197755894737105	5.44963785672555
4	3.6053347344917297	5.116830692253939
5	2.6114363962842413	4.632814595390131
6	2.2137792921460098	4.712821968884047
7	1.7351442078246113	4.309521760352367
8	1.3934906772020401	3.9553918798512506
9	1.2135596588094306	3.8752444569876054
>10	6.278505518574989	34.20265113057116
>50	0.046616083837019984	1.1070185323213644
>100	0.027564291743825183	1.944941021686159
>500	8.107144630536775E-4	0.17733581895578104
>1k	8.107144630536775E-4	0.5056875421318137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1790	0.2510279524533425	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1726	0.2420526513600386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008975301093303864	0.0
2	0.0	0.0	0.0	0.035620726214049714	0.0
3	0.0	0.0	0.0	0.04726056981942816	0.0
4	0.0	0.0	0.0	0.06731475819977899	0.0
5	0.0	0.0	0.0	0.11611795789461875	0.0
6	0.0	0.0	0.0	0.18217056437815188	0.0
7	0.0	0.0	0.0	0.22410204917343088	0.0
8	0.0	0.0	0.0	0.49153797393796944	0.0
9	0.0	0.0	0.0	0.606814497355091	0.0
10	0.0	0.0	0.0	0.7899667352903229	0.0
11	0.0	0.0	0.0	0.9512416768106268	0.0
12	0.0	0.0	0.0	1.0802616300268697	0.0
13	0.0	0.0	0.0	1.1398632388495908	0.0
14	0.0	0.0	0.0	1.1669293812090853	0.0
15	0.0	0.0	0.0	1.1984831741152318	0.0
16	0.0	0.0	0.0	1.2606090863704444	0.0
17	0.0	0.0	0.0	1.338301536459356	0.0
18	0.0	0.0	0.0	1.4645167080839414	0.0
19	0.0	0.0	0.0	1.519069710041679	0.0
20	0.0	0.0	0.0	1.5848418383660465	0.0
21	0.0	0.0	0.0	1.6727717412645078	0.0
22	0.0	0.0	0.0	1.7578968625713116	0.0
23	0.0	0.0	0.0	1.8618140205422204	0.0
24	0.0	0.0	0.0	1.9434331648594525	0.0
25	0.0	0.0	0.0	2.0040164472392537	0.0
26	0.0	0.0	0.0	2.0692276192452894	0.0
27	0.0	0.0	0.0	2.1354204648084054	0.0
28	0.0	0.0	0.0	2.2150762620114772	0.0
29	0.0	0.0	0.0	2.3004818614774467	0.0
30	0.0	0.0	0.0	2.4067830838012645	0.0
31	0.0	0.0	0.0	2.495694660256806	0.0
32	0.0	0.0	0.0	2.5843257585531814	0.0
33	0.0	0.0	0.0	2.675340921202466	0.0
34	0.0	0.0	0.0	2.77463018954714	0.0
35	0.0	0.0	0.0	2.8920102991580046	0.0
36	0.0	0.0	0.0	2.9859704824785296	0.0
37	0.0	0.0	0.0	3.084278077266123	0.0
38	0.0	0.0	0.0	3.1974510144895016	0.0
39	0.0	0.0	0.0	3.360549064044383	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	25	3.88931E-5	45.000004	21
GCGTTAA	25	3.88931E-5	45.000004	1
CGACTAG	25	3.88931E-5	45.000004	2
TACGTTC	20	7.0314837E-4	45.0	31
TGCGTAG	20	7.0314837E-4	45.0	2
TGCGATA	30	2.164339E-6	44.999996	1
CGTTTTA	1030	0.0	43.470875	1
CGTTTTT	1465	0.0	42.696243	1
TCACGAC	250	0.0	42.3	25
AGGCGAT	495	0.0	42.27273	7
AGGTACC	295	0.0	41.949154	7
CGGTCTA	245	0.0	41.32653	31
CGACGGT	240	0.0	41.249996	28
GACGGTC	240	0.0	41.249996	29
AAGGTAC	170	0.0	41.029415	6
TTTAGGC	165	0.0	40.90909	4
CGAAGGA	160	0.0	40.78125	4
ACGGTCT	245	0.0	40.408165	30
GTAAGGC	195	0.0	40.384617	4
TAGGCGA	140	0.0	40.178574	6
>>END_MODULE