FastQCFastQC Report
Thu 26 May 2016
SRR1545794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545794_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1074807
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.3511328080297207No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22090.2055252710486627No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17180.15984265082010074No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15400.14328153798775037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC750.044.00000410
TATTTCG207.856254E-444.010
TATCACC207.856254E-444.044
AACCGAT207.856254E-444.019
ATCTAGC254.4426462E-544.010
CACGGTA207.856254E-444.018
GCGAAAA254.4426462E-544.01
TTTCGTA254.4426462E-544.01
CGTTGAC254.4426462E-544.042
TAGCGAA800.044.01
AAGCGTA302.527564E-644.036
GTTACGT302.527564E-644.025
GTTACGA207.856254E-444.037
ACCTATC302.527564E-644.03
TACGCAA302.527564E-644.01
GTTCGAC207.856254E-444.038
TACGATT207.856254E-444.015
TGCGATA207.856254E-444.01
GTCGTAA408.310963E-944.01
TATTACG254.4426462E-544.01
Produced by FastQC (version 0.11.5)