##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545794_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1074807
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569062166509894	31.0	31.0	33.0	30.0	34.0
2	31.74877908312841	31.0	31.0	34.0	30.0	34.0
3	31.77476979587963	31.0	31.0	34.0	30.0	34.0
4	35.647390647809324	37.0	35.0	37.0	33.0	37.0
5	27.09064045917081	35.0	25.0	37.0	0.0	37.0
6	31.332384325744062	35.0	26.0	37.0	17.0	37.0
7	15.036825216062047	0.0	0.0	32.0	0.0	37.0
8	25.153241465677095	17.0	17.0	35.0	17.0	37.0
9	33.36492039966245	32.0	32.0	37.0	28.0	39.0
10	36.19137017157499	37.0	35.0	39.0	32.0	39.0
11	37.12583096314036	39.0	37.0	39.0	34.0	39.0
12	36.8380034741121	39.0	35.0	39.0	33.0	39.0
13	36.182426240245924	38.0	35.0	39.0	32.0	39.0
14	37.480872379878434	40.0	35.0	41.0	33.0	41.0
15	37.838559853071295	40.0	35.0	41.0	33.0	41.0
16	37.96847899204229	40.0	35.0	41.0	33.0	41.0
17	38.00803307012329	40.0	35.0	41.0	34.0	41.0
18	37.98725724711506	40.0	36.0	41.0	34.0	41.0
19	37.90961912231684	40.0	36.0	41.0	34.0	41.0
20	37.904357712593985	40.0	35.0	41.0	34.0	41.0
21	37.73495520591139	40.0	35.0	41.0	33.0	41.0
22	37.65524601160953	39.0	35.0	41.0	33.0	41.0
23	37.56550617924893	39.0	35.0	41.0	33.0	41.0
24	37.432324128890116	39.0	35.0	41.0	33.0	41.0
25	37.24999092860393	39.0	35.0	41.0	33.0	41.0
26	37.026700607643974	38.0	35.0	40.0	33.0	41.0
27	36.75694147879573	38.0	35.0	40.0	32.0	41.0
28	36.83505782898697	38.0	35.0	40.0	32.0	41.0
29	36.85270750934819	38.0	35.0	40.0	32.0	41.0
30	36.63615700307125	38.0	35.0	40.0	32.0	41.0
31	36.396732622694124	38.0	35.0	40.0	31.0	41.0
32	36.11663489352042	38.0	35.0	40.0	31.0	41.0
33	35.90358268972941	38.0	35.0	40.0	30.0	41.0
34	35.70834949902634	38.0	35.0	40.0	30.0	41.0
35	35.55357566521245	38.0	35.0	40.0	29.0	41.0
36	35.34965812466796	38.0	34.0	40.0	29.0	41.0
37	35.13792615790556	38.0	34.0	40.0	27.0	41.0
38	34.99135472694167	37.0	34.0	40.0	27.0	41.0
39	35.008568980291344	38.0	34.0	40.0	27.0	41.0
40	34.736182402980255	37.0	34.0	40.0	26.0	41.0
41	34.59317440247412	37.0	34.0	40.0	25.0	40.0
42	34.59973465003484	37.0	34.0	40.0	25.0	41.0
43	34.49053923169462	37.0	33.0	40.0	25.0	41.0
44	34.270545316507985	36.0	33.0	40.0	24.0	40.0
45	34.01107547680653	36.0	33.0	39.0	23.0	40.0
46	34.0069854401767	36.0	33.0	39.0	23.0	40.0
47	33.86522045353259	36.0	33.0	39.0	23.0	40.0
48	33.838223978816664	36.0	33.0	39.0	23.0	40.0
49	33.77041924736255	36.0	33.0	39.0	24.0	40.0
50	33.782837290788024	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	5.0
15	21.0
16	45.0
17	152.0
18	378.0
19	835.0
20	1561.0
21	2578.0
22	4080.0
23	6324.0
24	9858.0
25	14281.0
26	18031.0
27	19289.0
28	19744.0
29	22162.0
30	26927.0
31	36772.0
32	52344.0
33	103006.0
34	96307.0
35	112598.0
36	130268.0
37	252163.0
38	119109.0
39	25967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.918760298360542	23.821579130020552	29.419886547073105	15.839774024545802
2	32.856596579664995	24.787985191760008	29.870944271855322	12.484473956719672
3	28.849923753753	23.11680143504834	34.76940511180147	13.263869699397194
4	25.008117736486646	27.28685243025027	33.15144021205668	14.553589621206411
5	17.07664724922707	47.681955923249475	24.583669440187865	10.657727387335587
6	19.250432868412652	40.38492492140449	29.85363883934511	10.511003370837741
7	39.56263775729038	55.673809344375314	3.956338207696824	0.807214690637482
8	88.87716585396261	2.1937892105280294	7.532236020048251	1.3968089154611014
9	85.85662356125331	3.4874168106460046	8.666951368943447	1.9890082591572253
10	56.43738829389835	22.10145635448969	12.810392935661937	8.650762415950027
11	52.77207908024417	16.177136918535144	19.36682585803777	11.683958143182915
12	46.08334333512901	19.287555812345843	22.637552602467235	11.991548250057917
13	19.509176996428195	45.65424304084361	23.219238430713606	11.617341532014585
14	13.265637458632106	47.3030041672598	27.648405713770007	11.78295266033809
15	11.212059467420662	21.13039829476362	55.198468190102965	12.459074047712752
16	13.28266377126312	14.905745868793188	53.82743134348772	17.984159016455976
17	13.912916458489757	15.026790856404917	29.708217382283518	41.35207530282181
18	20.1482684798294	21.73339027378869	37.68564960965085	20.43269163673106
19	29.2755815695283	22.562748474842458	26.787413926407254	21.374256029221993
20	31.632004629668398	21.555125710941592	25.488017848785876	21.324851810604137
21	20.419014762650413	28.096672239760256	30.737611496761748	20.74670150082759
22	22.19961351200727	22.227246380047767	26.77029457381651	28.80284553412845
23	17.423500219109105	30.892243909836836	25.05277691715815	26.63147895389591
24	18.6852151130389	22.42477021455945	42.35318526954141	16.536829402860235
25	15.844612102451883	22.70323881403824	40.98940553978528	20.462743543724592
26	15.358106153011658	35.06071322572332	28.252421132352133	21.328759488912894
27	16.334653570361933	34.94925135396401	30.461654976195728	18.254440099478327
28	13.521125188057018	27.62672740315238	42.65389041939623	16.198256989394373
29	14.635650865690305	23.163135334995026	40.91981164990552	21.28140214940915
30	17.39949590949817	29.509111868456383	34.55141248614868	18.539979735896768
31	31.146242999906033	24.522635226603473	25.387534692274986	18.94358708121551
32	31.37270226189446	24.669824442899984	27.8635141006711	16.09395919453446
33	28.87048558485384	25.231320599884445	26.168419074308225	19.72977474095349
34	18.557285168406978	26.821838711508207	29.419700467153636	25.20117565293118
35	19.11710660611626	23.842699200879785	33.14827685342578	23.891917339578175
36	33.845518311659674	20.64538098467911	27.554714474319574	17.95438622934164
37	20.073371312244895	31.40228896908933	30.097031374004825	18.427308344660947
38	19.829606617746258	33.03914098065978	23.90410557430311	23.227146827290852
39	19.98312255130456	30.526038628330483	27.879982173543716	21.610856646821244
40	24.11046820498936	24.47248668830776	25.718756948921996	25.698288157780887
41	16.847582868366136	22.084988281617072	28.56661707636813	32.500811773648664
42	22.59987141877565	24.72713705809508	24.281010451178677	28.39198107195059
43	22.686119461447497	25.143304797977684	26.417952246310268	25.752623494264554
44	19.77582952102098	30.33093383277184	28.99841552948576	20.89482111672142
45	16.906198042997485	38.562458190168094	22.244086612759315	22.287257154075103
46	23.59223562928042	30.97551467379725	27.114821544705237	18.317428152217097
47	22.600987898292438	26.846215180958072	26.34780011667211	24.204996804077382
48	23.789945543711568	23.174765329961566	30.68039192152638	22.35489720480049
49	21.44440815886015	21.6510499094256	33.53188060740207	23.37266132431218
50	20.533826072960075	32.073758358477384	26.740242666822972	20.65217290173957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	740.0
1	1069.5
2	1399.0
3	6191.5
4	10984.0
5	8253.5
6	5523.0
7	6056.0
8	6589.0
9	7198.5
10	7808.0
11	7976.0
12	8144.0
13	7541.5
14	6939.0
15	6448.5
16	5958.0
17	5405.5
18	4853.0
19	4613.5
20	4374.0
21	4338.0
22	4302.0
23	4568.5
24	4835.0
25	5566.5
26	6298.0
27	6961.5
28	7625.0
29	8900.0
30	10175.0
31	12575.0
32	14975.0
33	17055.0
34	19135.0
35	20152.0
36	21169.0
37	27738.0
38	34307.0
39	54329.5
40	74352.0
41	95709.5
42	117067.0
43	120829.0
44	124591.0
45	119787.0
46	114983.0
47	107107.5
48	99232.0
49	93538.0
50	87844.0
51	77151.5
52	66459.0
53	58657.0
54	50855.0
55	45260.5
56	39666.0
57	35874.0
58	32082.0
59	28733.0
60	25384.0
61	22229.0
62	19074.0
63	16166.5
64	13259.0
65	11006.5
66	8754.0
67	7377.5
68	6001.0
69	5104.5
70	4208.0
71	3316.0
72	2424.0
73	1850.5
74	1277.0
75	964.0
76	651.0
77	465.5
78	280.0
79	211.5
80	143.0
81	100.5
82	58.0
83	35.5
84	13.0
85	10.5
86	8.0
87	5.0
88	2.0
89	2.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1074807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.120902330470734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27707151422437	15.687987728453232
2	6.179390090765558	2.610285891378761
3	2.6198305606873316	1.6599955618397852
4	1.6614312694273312	1.403637102814587
5	1.207297137274659	1.2749602460117497
6	0.9618507990990722	1.2189094070554038
7	0.8242232628684553	1.2185837323482585
8	0.6814360817002137	1.1514035940839111
9	0.6432900177832805	1.2228179072190706
>10	9.240464252113563	44.891069480570515
>50	1.5280410166917602	21.428641478193317
>100	0.17336856432815037	5.2705114850148105
>500	4.610866072557191E-4	0.061255575093760464
>1k	0.0018443464290228764	0.8999408099227988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3774	0.3511328080297207	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2209	0.2055252710486627	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1718	0.15984265082010074	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1540	0.14328153798775037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011071755208144346	0.0
2	0.0	0.0	0.0	0.05470749632259559	0.0
3	0.0	0.0	0.0	0.07666492681941968	0.0
4	0.0	0.0	0.0	0.11509043018886181	0.0
5	0.0	0.0	0.0	0.18766159878006006	0.0
6	0.0	0.0	0.0	0.32005746147913067	0.0
7	0.0	0.0	0.0	0.40044398668784253	0.0
8	0.0	0.0	0.0	0.8027487725703313	0.0
9	0.0	0.0	0.0	0.9710580597260717	0.0
10	0.0	0.0	0.0	1.240408743151096	0.0
11	0.0	0.0	0.0	1.5070612677438833	0.0
12	0.0	0.0	0.0	1.7380794877591976	0.0
13	0.0	0.0	0.0	1.8352132057197246	0.0
14	0.0	0.0	0.0	1.8638695133172747	0.0
15	0.0	0.0	0.0	1.9154136510089719	0.0
16	0.0	0.0	0.0	2.0423201560838367	0.0
17	0.0	0.0	0.0	2.1869972934675714	0.0
18	0.0	0.0	0.0	2.4215510319527134	0.0
19	0.0	0.0	0.0	2.505566115590985	0.0
20	0.0	0.0	0.0	2.5959079164910537	0.0
21	0.0	0.0	0.0	2.7189067432571616	0.0
22	0.0	0.0	0.0	2.8315781344929833	0.0
23	0.0	0.0	0.0	2.9810003098230657	0.0
24	0.0	0.0	0.0	3.0734820297969776	0.0
25	0.0	0.0	0.0	3.1484722373412155	0.0
26	0.0	0.0	0.0	3.228021402912337	0.0
27	0.0	0.0	0.0	3.303848970094166	0.0
28	0.0	0.0	0.0	3.3847937350612716	0.0
29	0.0	0.0	0.0	3.4785780144714353	0.0
30	0.0	0.0	0.0	3.5994369221637	0.0
31	0.0	0.0	0.0	3.711829193520325	0.0
32	0.0	0.0	0.0	3.8017988345814646	0.0
33	0.0	0.0	0.0	3.896792633468148	0.0
34	0.0	0.0	0.0	3.996624510260912	0.0
35	0.0	0.0	0.0	4.143255486799025	0.0
36	0.0	0.0	0.0	4.246529842101884	0.0
37	0.0	0.0	0.0	4.3536188357537675	0.0
38	0.0	0.0	0.0	4.456055831419036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATC	75	0.0	44.000004	10
TATTTCG	20	7.856254E-4	44.0	10
TATCACC	20	7.856254E-4	44.0	44
AACCGAT	20	7.856254E-4	44.0	19
ATCTAGC	25	4.4426462E-5	44.0	10
CACGGTA	20	7.856254E-4	44.0	18
GCGAAAA	25	4.4426462E-5	44.0	1
TTTCGTA	25	4.4426462E-5	44.0	1
CGTTGAC	25	4.4426462E-5	44.0	42
TAGCGAA	80	0.0	44.0	1
AAGCGTA	30	2.527564E-6	44.0	36
GTTACGT	30	2.527564E-6	44.0	25
GTTACGA	20	7.856254E-4	44.0	37
ACCTATC	30	2.527564E-6	44.0	3
TACGCAA	30	2.527564E-6	44.0	1
GTTCGAC	20	7.856254E-4	44.0	38
TACGATT	20	7.856254E-4	44.0	15
TGCGATA	20	7.856254E-4	44.0	1
GTCGTAA	40	8.310963E-9	44.0	1
TATTACG	25	4.4426462E-5	44.0	1
>>END_MODULE