FastQCFastQC Report
Thu 26 May 2016
SRR1545793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545793_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences414284
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.24234583039653956No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.2235181662820674No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9000.21724227824391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG551.8189894E-1245.05
TCGTTAG207.028057E-445.02
GATCGAC253.8864713E-545.09
ACGCATG207.028057E-445.01
CTCCCGT253.8864713E-545.036
GTTAGGT253.8864713E-545.04
TTCCGTC253.8864713E-545.027
TAACGCC351.2094097E-745.012
ACGGGCT207.028057E-445.05
ATATCCG253.8864713E-545.018
CTTGCGG453.8380676E-1045.02
TGCGACA207.028057E-445.01
ACGGAGA207.028057E-445.024
AGCGAAC207.028057E-445.030
GATGCAC253.8864713E-545.019
GCTAGGC351.2094097E-745.04
TCGTAGC207.028057E-445.033
CGTAAAT207.028057E-445.016
CGGTCTA1750.045.031
TAAGCCG207.028057E-445.018