FastQCFastQC Report
Thu 26 May 2016
SRR1545792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545792_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences783078
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.28873241235228164No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18890.24122756609175589No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.12272085283969157No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.11454797606368715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAT254.4415356E-544.039
GCGACTA207.8549434E-444.01
CGTATGG408.307325E-944.02
CGCATAT207.8549434E-444.041
ATTAACG207.8549434E-444.01
CGATTTA207.8549434E-444.013
TATGCTA302.5266781E-644.01
CCTAATC207.8549434E-444.029
TTATCGC207.8549434E-444.036
ACGGGTA254.4415356E-544.05
CGTTATT9800.044.01
ATACTCG207.8549434E-444.044
CATTACC254.4415356E-544.014
TGCGATG302.5266781E-644.01
TATTACG351.4452962E-744.01
TGTTACG302.5266781E-644.01
GAACGTT351.4452962E-744.022
TTGCGAA254.4415356E-544.01
GCGATAC302.5266781E-644.09
CGTGTTG207.8549434E-444.020
Produced by FastQC (version 0.11.5)