FastQCFastQC Report
Thu 26 May 2016
SRR1545791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545791_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences302619
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7550.24948863091874604No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.20884346323264566No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.167537398510999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA351.2079545E-745.00000424
CGTTGAT351.2079545E-745.00000425
AGTGCAA351.2079545E-745.0000041
TGAGGTA351.2079545E-745.0000045
CGGTCTA351.2079545E-745.00000431
TCGCGGG351.2079545E-745.0000043
TTTACGG207.0250407E-445.02
TAGGTTA207.0250407E-445.06
ATTGTTA207.0250407E-445.01
ATTCGAA207.0250407E-445.01
AAGGTAT502.1827873E-1145.06
GGATATC253.8839724E-545.08
TAACGGG551.8189894E-1245.03
TATGCCA406.7866495E-945.010
CCGCTCG207.0250407E-445.019
TGCATAG551.8189894E-1245.02
ACTAGGT207.0250407E-445.04
GTAGCCG253.8839724E-545.043
TACGCAA207.0250407E-445.01
GTTCGCT207.0250407E-445.038