##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545791_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	302619
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01185979730288	31.0	31.0	33.0	30.0	33.0
2	31.166608838176057	31.0	31.0	33.0	30.0	34.0
3	31.101460912897075	31.0	31.0	34.0	28.0	34.0
4	35.06495956962385	35.0	35.0	37.0	32.0	37.0
5	32.791001225964	35.0	35.0	37.0	28.0	37.0
6	33.97321053866413	35.0	35.0	37.0	28.0	37.0
7	15.554333997534854	0.0	0.0	35.0	0.0	37.0
8	25.053542573334788	17.0	17.0	35.0	17.0	37.0
9	32.847970550428094	32.0	32.0	37.0	27.0	39.0
10	35.61957775288399	37.0	35.0	37.0	32.0	39.0
11	36.517535250595635	37.0	35.0	39.0	32.0	39.0
12	36.12503511015501	37.0	35.0	39.0	32.0	39.0
13	35.483356960402354	37.0	35.0	39.0	30.0	39.0
14	36.48224665338264	38.0	35.0	40.0	31.0	41.0
15	37.00321856856311	39.0	35.0	40.0	32.0	41.0
16	37.18420852623265	39.0	35.0	41.0	32.0	41.0
17	37.179473198972964	39.0	35.0	41.0	32.0	41.0
18	37.097921809271725	38.0	35.0	40.0	32.0	41.0
19	37.00218756918766	38.0	35.0	40.0	32.0	41.0
20	37.08144564617555	39.0	35.0	40.0	32.0	41.0
21	36.8738942366474	38.0	35.0	40.0	31.0	41.0
22	36.93824578099855	39.0	35.0	40.0	32.0	41.0
23	36.97017041230062	38.0	35.0	40.0	32.0	41.0
24	36.803746625294515	38.0	35.0	40.0	32.0	41.0
25	36.570730852986756	38.0	35.0	40.0	31.0	41.0
26	36.43176733780761	38.0	35.0	40.0	31.0	41.0
27	36.30696023712986	38.0	35.0	40.0	31.0	41.0
28	36.268092882469375	38.0	35.0	40.0	31.0	41.0
29	36.3356530819281	38.0	35.0	40.0	31.0	41.0
30	36.11298034822665	38.0	35.0	40.0	30.0	41.0
31	35.95182391059385	38.0	34.0	40.0	30.0	41.0
32	35.78275983993074	38.0	34.0	40.0	30.0	41.0
33	35.58465264904054	38.0	34.0	40.0	29.0	41.0
34	35.46987466087721	38.0	34.0	40.0	28.0	41.0
35	35.319636242271635	38.0	34.0	40.0	27.0	41.0
36	35.08899639480667	38.0	34.0	40.0	26.0	41.0
37	34.92965081505127	37.0	34.0	40.0	26.0	41.0
38	34.87682531500005	37.0	34.0	40.0	26.0	41.0
39	34.8421381340894	37.0	34.0	40.0	25.0	41.0
40	34.68151702305539	37.0	33.0	40.0	24.0	41.0
41	34.57308034194813	37.0	34.0	40.0	24.0	41.0
42	34.525594889944124	37.0	33.0	40.0	24.0	41.0
43	34.41728708375879	37.0	33.0	40.0	23.0	41.0
44	34.22191931108093	36.0	33.0	40.0	23.0	41.0
45	34.09860914218869	36.0	33.0	40.0	23.0	41.0
46	34.10694305380693	36.0	33.0	40.0	23.0	41.0
47	34.05284863144746	36.0	33.0	40.0	23.0	41.0
48	34.09959387877166	36.0	33.0	40.0	23.0	41.0
49	34.01981699761086	36.0	33.0	40.0	24.0	41.0
50	33.851714532134466	36.0	33.0	40.0	23.0	41.0
51	33.63784825143167	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	8.0
15	18.0
16	28.0
17	102.0
18	222.0
19	377.0
20	658.0
21	1026.0
22	1618.0
23	2296.0
24	3310.0
25	4409.0
26	5211.0
27	5703.0
28	6392.0
29	7397.0
30	9171.0
31	11928.0
32	16048.0
33	24925.0
34	32012.0
35	31132.0
36	32627.0
37	50299.0
38	47425.0
39	8274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.13374242859833	23.864000608025275	29.402978662939205	13.599278300437184
2	32.50423800224044	23.396746403894007	31.422680003568843	12.67633559029671
3	29.651806396822405	23.262253857160324	34.1072437619581	12.978695984059163
4	26.35326929241059	27.410374100766973	31.10974525723765	15.126611349584792
5	20.924330593915123	37.57265736784537	28.01872982198738	13.484282216252119
6	20.397926105102457	41.051288914443575	27.98535452169229	10.565430458761679
7	39.424490861446245	56.16963905108404	3.7218416556792535	0.6840284317904692
8	90.67507327695881	2.2265621127556434	6.047538323766849	1.0508262865186917
9	87.36761406256713	3.6547606065712994	6.988325253867074	1.9893000769945046
10	59.35384096834634	19.6299637497976	11.559750048741156	9.456445233114907
11	54.60364352535697	16.60305532699533	17.53260700749127	11.260694140156433
12	48.494311328766535	19.853346947812263	20.68872080074285	10.96362092267835
13	21.164897114853993	45.734074859807215	20.839735773365188	12.261292251973604
14	13.666689798062912	47.81061334549384	25.797454885516114	12.72524197092714
15	11.884250493194413	21.312277153780826	53.23195172808053	13.571520624944236
16	13.083778612711033	14.83713844801549	52.85127503560582	19.22780790366765
17	14.232748109008355	14.997736427653255	28.846833807526956	41.92268165581143
18	20.82023931081657	21.157627247462983	36.346693366906905	21.675440074813544
19	31.427967179853216	22.834653475161836	24.032198903571818	21.70518044141313
20	34.74996612902693	21.16985384262059	23.182946212894763	20.89723381545772
21	22.02670684920643	29.420492434381185	26.68768319239704	21.865117524015346
22	22.959563014880096	23.428799910118002	24.139925120365874	29.471711954636028
23	18.32403120755802	31.87440312736477	23.458209828199816	26.343355836877393
24	18.980301963855542	22.324110515202282	42.29311444423516	16.402473076707015
25	16.953330755834894	22.47677773041349	39.19119420789838	21.378697305853233
26	15.305053549182304	36.91109943526348	26.68107422204158	21.102772793512635
27	16.58950693776663	36.10150056671921	28.739768487768448	18.569224007745714
28	13.905604076412914	27.255063297413578	42.698574775542845	16.14075785063066
29	14.370545140919772	22.2672733701453	41.41907811472512	21.943103374209816
30	17.541859565988915	31.508266169672094	32.23954873950413	18.710325524834857
31	33.25501703462109	25.365228224268797	23.007478049957207	18.3722766911529
32	33.99356947184413	24.99578678139839	24.652450771432065	16.35819297532541
33	30.527494968921314	26.50197112540852	23.469775526321875	19.50075837934829
34	18.747005310307678	27.07298616412056	27.496951612423544	26.683056913148217
35	19.703323320743245	23.888123349822717	32.77355354422558	23.634999785208464
36	33.95358520119358	23.125778619320002	24.81205740551651	18.10857877396991
37	20.634196795310274	33.26360869608319	27.884567723771475	18.217626784835055
38	19.807745052359568	35.116103086719605	21.767304762754485	23.30884709816634
39	20.29846110125273	33.18892733106646	25.8407436413444	20.671867926336414
40	25.446518559641003	25.16927225322931	23.924803135295537	25.459406051834154
41	17.283448825090293	22.5246927654906	27.211774541585292	32.98008386783381
42	21.92492870573229	24.8811211457311	22.253724980916598	30.94022516762001
43	22.1760695792399	25.449823044818732	24.378839398715876	27.99526797722549
44	19.39765844180306	29.199752824508707	28.632703168009936	22.769885565678294
45	16.153314894306043	39.898023587415196	21.75838265277461	22.190278865504148
46	22.940727449367028	34.06263321205873	24.418162772330884	18.578476566243364
47	21.250483280957244	27.31553537616607	25.77432348927199	25.6596578536047
48	22.570955557978845	23.021356887703682	31.611035658699553	22.796651895617924
49	20.2495547206223	23.220947792438675	34.16540270108619	22.36409478585284
50	18.563275934425796	34.449588426371115	26.56706948340983	20.420066155793258
51	17.598035814010355	36.63286178329847	22.588469329420825	23.18063307327035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	140.0
1	208.5
2	277.0
3	1266.5
4	2256.0
5	1770.5
6	1285.0
7	1366.0
8	1447.0
9	1534.0
10	1621.0
11	1589.0
12	1557.0
13	1494.5
14	1432.0
15	1316.0
16	1200.0
17	1132.0
18	1064.0
19	1046.5
20	1029.0
21	988.5
22	948.0
23	951.0
24	954.0
25	1155.0
26	1719.0
27	2082.0
28	2502.5
29	2923.0
30	3496.0
31	4069.0
32	4468.0
33	4867.0
34	5411.0
35	5955.0
36	7232.5
37	8510.0
38	12537.0
39	16564.0
40	22245.5
41	27927.0
42	30976.0
43	34025.0
44	33030.5
45	32036.0
46	29551.0
47	27066.0
48	24922.5
49	22779.0
50	21622.5
51	20466.0
52	18622.0
53	16778.0
54	15145.5
55	13513.0
56	12342.5
57	11172.0
58	10538.5
59	9905.0
60	9004.5
61	8104.0
62	6914.5
63	5725.0
64	4865.0
65	4005.0
66	3346.5
67	2688.0
68	2339.0
69	1990.0
70	1623.0
71	1256.0
72	1013.0
73	770.0
74	624.0
75	353.0
76	228.0
77	161.0
78	94.0
79	68.0
80	42.0
81	32.5
82	23.0
83	15.0
84	7.0
85	4.5
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.004662628585777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.17221239315329	20.82651783265426
2	7.799543836463241	4.836444506128168
3	4.378317310766739	4.07244753303659
4	3.0982883209344956	3.8424553646664616
5	2.3671476989320657	3.6696307898710923
6	1.880075885149106	3.497467112111269
7	1.6274806556817938	3.532164206477452
8	1.3610299916867392	3.3758620575707408
9	1.2405942915609747	3.461778672191766
>10	8.998571824440987	46.71980278832459
>50	0.068211369982734	1.350212643621187
>100	0.005329013279901094	0.18934700068402843
>500	0.003197407967940656	0.6258694926623907
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	755	0.24948863091874604	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	632	0.20884346323264566	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.167537398510999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006939418873236644	0.0
2	0.0	0.0	0.0	0.034036197330636876	0.0
3	0.0	0.0	0.0	0.04130606472164669	0.0
4	0.0	0.0	0.0	0.06344611541244932	0.0
5	0.0	0.0	0.0	0.10376083458077649	0.0
6	0.0	0.0	0.0	0.15332811224675252	0.0
7	0.0	0.0	0.0	0.18439027291743082	0.0
8	0.0	0.0	0.0	0.3724154795303666	0.0
9	0.0	0.0	0.0	0.43487024938949637	0.0
10	0.0	0.0	0.0	0.5402833265591387	0.0
11	0.0	0.0	0.0	0.6757672188461399	0.0
12	0.0	0.0	0.0	0.7722581860359066	0.0
13	0.0	0.0	0.0	0.8165382874175118	0.0
14	0.0	0.0	0.0	0.8287648825751192	0.0
15	0.0	0.0	0.0	0.8548703154791999	0.0
16	0.0	0.0	0.0	0.9249254012471128	0.0
17	0.0	0.0	0.0	1.011172464385911	0.0
18	0.0	0.0	0.0	1.1509521874039634	0.0
19	0.0	0.0	0.0	1.1939104947144759	0.0
20	0.0	0.0	0.0	1.2332338683294837	0.0
21	0.0	0.0	0.0	1.3132024096305916	0.0
22	0.0	0.0	0.0	1.3905273627895143	0.0
23	0.0	0.0	0.0	1.4906532636747858	0.0
24	0.0	0.0	0.0	1.5550907246405545	0.0
25	0.0	0.0	0.0	1.6039971052709843	0.0
26	0.0	0.0	0.0	1.664469184023475	0.0
27	0.0	0.0	0.0	1.7180018439027291	0.0
28	0.0	0.0	0.0	1.7665777760153858	0.0
29	0.0	0.0	0.0	1.82638895773233	0.0
30	0.0	0.0	0.0	1.9136273664244479	0.0
31	0.0	0.0	0.0	1.997891738456607	0.0
32	0.0	0.0	0.0	2.0725731034733443	0.0
33	0.0	0.0	0.0	2.1432890862768037	0.0
34	0.0	0.0	0.0	2.2169791057402213	0.0
35	0.0	0.0	0.0	2.329001153265327	0.0
36	0.0	0.0	0.0	2.4112828341908474	0.0
37	0.0	0.0	0.0	2.4879468903142237	0.0
38	0.0	0.0	0.0	2.561306461259868	0.0
39	0.0	0.0	0.0	2.643588142185388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	35	1.2079545E-7	45.000004	24
CGTTGAT	35	1.2079545E-7	45.000004	25
AGTGCAA	35	1.2079545E-7	45.000004	1
TGAGGTA	35	1.2079545E-7	45.000004	5
CGGTCTA	35	1.2079545E-7	45.000004	31
TCGCGGG	35	1.2079545E-7	45.000004	3
TTTACGG	20	7.0250407E-4	45.0	2
TAGGTTA	20	7.0250407E-4	45.0	6
ATTGTTA	20	7.0250407E-4	45.0	1
ATTCGAA	20	7.0250407E-4	45.0	1
AAGGTAT	50	2.1827873E-11	45.0	6
GGATATC	25	3.8839724E-5	45.0	8
TAACGGG	55	1.8189894E-12	45.0	3
TATGCCA	40	6.7866495E-9	45.0	10
CCGCTCG	20	7.0250407E-4	45.0	19
TGCATAG	55	1.8189894E-12	45.0	2
ACTAGGT	20	7.0250407E-4	45.0	4
GTAGCCG	25	3.8839724E-5	45.0	43
TACGCAA	20	7.0250407E-4	45.0	1
GTTCGCT	20	7.0250407E-4	45.0	38
>>END_MODULE