FastQCFastQC Report
Thu 26 May 2016
SRR1545790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545790_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1224814
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48340.39467217063162247No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.17063815403808252No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.15422749903250618No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15570.12712134250588253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA207.856685E-444.012
CTATACG207.856685E-444.01
TCGCTAA207.856685E-444.017
AATTCGC700.044.013
CATACGT207.856685E-444.030
ATCGGTA207.856685E-444.044
CGCAATA207.856685E-444.033
CGATACG302.5278532E-644.012
CGTTTTA25350.043.5660741
TTACGGG2800.041.6428573
CGCATGG750.041.0666662
CGTTATT14950.041.0568581
TAGGCCG700.040.8571436
GTTTTAT29950.040.6210372
ACACGAC1200.040.33333226
TGCGTAA603.6379788E-1240.3333321
CACGACC1150.040.17391627
CGACTAG557.8216544E-1140.0000042
GTACGGG3550.039.6619723
TTACTGG18500.039.4810840
Produced by FastQC (version 0.11.5)