##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545790_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1224814
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519439686352378	31.0	31.0	33.0	30.0	34.0
2	31.705140535624185	31.0	31.0	34.0	30.0	34.0
3	31.755915592081738	31.0	31.0	34.0	30.0	34.0
4	35.65644987728749	37.0	35.0	37.0	33.0	37.0
5	27.16309660079	35.0	26.0	37.0	0.0	37.0
6	31.367510495471148	35.0	28.0	37.0	17.0	37.0
7	14.953526821215302	0.0	0.0	32.0	0.0	37.0
8	25.080700416553043	17.0	17.0	35.0	17.0	37.0
9	33.21315889596298	32.0	32.0	37.0	28.0	39.0
10	36.008874816910975	37.0	35.0	39.0	32.0	39.0
11	36.99126397967365	39.0	37.0	39.0	34.0	39.0
12	37.01660252087256	39.0	35.0	39.0	33.0	39.0
13	36.57020331250296	39.0	35.0	39.0	33.0	39.0
14	37.85137743363482	40.0	36.0	41.0	33.0	41.0
15	38.13407178559357	40.0	37.0	41.0	33.0	41.0
16	38.19303175829146	40.0	36.0	41.0	33.0	41.0
17	38.20794667598509	40.0	36.0	41.0	34.0	41.0
18	38.198828556825774	40.0	36.0	41.0	34.0	41.0
19	38.14796532371446	40.0	36.0	41.0	34.0	41.0
20	38.182919202425836	40.0	36.0	41.0	34.0	41.0
21	38.04778439828415	40.0	36.0	41.0	34.0	41.0
22	37.96943290981324	40.0	36.0	41.0	34.0	41.0
23	37.883503127821854	40.0	36.0	41.0	34.0	41.0
24	37.74107415493291	40.0	35.0	41.0	33.0	41.0
25	37.56059777239646	39.0	35.0	41.0	33.0	41.0
26	37.33410052465109	39.0	35.0	40.0	33.0	41.0
27	37.11840491699148	39.0	35.0	40.0	33.0	41.0
28	37.15749493392466	39.0	35.0	40.0	33.0	41.0
29	37.07525550818328	39.0	35.0	40.0	32.0	41.0
30	36.870088029692674	39.0	35.0	40.0	32.0	41.0
31	36.7097151077633	38.0	35.0	40.0	31.0	41.0
32	36.49939011147815	38.0	35.0	40.0	31.0	41.0
33	36.30712500020411	38.0	35.0	40.0	31.0	41.0
34	36.08516476787496	38.0	35.0	40.0	30.0	41.0
35	35.88593696675577	38.0	35.0	40.0	30.0	41.0
36	35.70333944582606	38.0	35.0	40.0	29.0	41.0
37	35.58628085570544	38.0	35.0	40.0	29.0	41.0
38	35.397359925670344	38.0	34.0	40.0	28.0	41.0
39	35.3392678398516	38.0	34.0	40.0	28.0	41.0
40	35.10062670740211	38.0	34.0	40.0	27.0	41.0
41	34.881501191201274	38.0	34.0	40.0	26.0	40.0
42	34.897341963759395	38.0	34.0	40.0	26.0	41.0
43	34.782680472300285	37.0	34.0	40.0	26.0	41.0
44	34.581649948481974	37.0	34.0	40.0	26.0	40.0
45	34.34581087414089	37.0	33.0	40.0	25.0	40.0
46	34.28904470393056	37.0	33.0	39.0	24.0	40.0
47	34.13349618799263	36.0	33.0	39.0	24.0	40.0
48	34.01893675284574	36.0	33.0	39.0	24.0	40.0
49	33.91055376571463	36.0	33.0	39.0	24.0	40.0
50	33.98052765562771	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	5.0
14	16.0
15	34.0
16	76.0
17	180.0
18	369.0
19	834.0
20	1560.0
21	2605.0
22	4059.0
23	6463.0
24	10000.0
25	14046.0
26	18208.0
27	20163.0
28	21471.0
29	24439.0
30	29818.0
31	39573.0
32	55017.0
33	95851.0
34	102033.0
35	128414.0
36	172928.0
37	308102.0
38	134178.0
39	34369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.607948635466286	23.029129320860147	27.686326250353115	14.676595793320454
2	34.49380885587526	23.198297864002207	30.03574420279324	12.272149077329292
3	30.648571946434316	23.24475389732645	33.262111634909466	12.844562521329769
4	27.12420008262479	26.877060516943796	30.746382716069544	15.252356684361871
5	17.33855099631454	47.88751598201849	23.556229762233286	11.217703259433677
6	20.38415628822009	41.898443355480914	28.099940072533464	9.617460283765535
7	39.36842655292967	55.82210849973956	3.8669544926821544	0.9425104546486242
8	87.76589751586772	2.6635064589398882	7.611196475546492	1.9593995496459053
9	83.61408344450668	4.335760368513097	9.240015218637279	2.8101409683429486
10	46.38581858143359	26.173933348247164	15.029547343515015	12.410700726804233
11	40.58436627928812	22.405606075698024	22.126543295553446	14.883484349460407
12	36.70238909744663	21.29547833385314	26.63824874634026	15.363883822359966
13	23.55916898402533	32.6873304844654	28.083611062577663	15.669889468931608
14	18.371524166118284	35.877202579330415	29.998187479894906	15.753085774656398
15	16.671021069321547	23.338890639721622	43.31637293499258	16.67371535596425
16	19.207651120904888	18.56061410140642	41.96629039184725	20.26544438584144
17	19.54517175669122	18.862129270240217	30.173805981969508	31.418892991099057
18	22.90159975310537	22.928787554681772	33.25166106853775	20.917951623675105
19	28.632837312440913	24.512130005045666	26.6528632102507	20.202169472262728
20	29.648420086641725	23.704007302333252	25.420022958587996	21.227549652437023
21	24.80997114663941	25.820573572803706	29.76190670583452	19.607548574722365
22	23.349096270944	22.667605040438794	27.382688310225063	26.600610378392147
23	20.878598709681633	28.195464780774877	26.59554838530585	24.33038812423764
24	21.255472259461435	25.10781228823315	34.83541174415054	18.80130370815487
25	21.196524533521007	23.656898108610775	33.34571616588315	21.800861191985067
26	19.21467259518588	29.407567189793717	27.964327644850567	23.41343257016984
27	19.330363630722704	28.920554467862058	30.393349520825204	21.35573238059003
28	18.33772311550978	26.08689972518276	36.25276980831375	19.3226073509937
29	18.858210307850825	24.76947520194903	35.07863234744214	21.293682142758
30	21.73954575960105	26.228472241499524	32.28816783609593	19.743814162803496
31	28.992565401767123	23.994010519148212	26.590404747169778	20.423019331914887
32	28.513962119962706	24.719345141384732	27.64199298832313	19.124699750329437
33	27.27418203906879	23.809411061597924	27.086561714676677	21.82984518465661
34	21.657900709822062	25.94777656035937	28.900959655915102	23.493363073903467
35	21.03935781269646	24.647579142629002	30.97147811831021	23.341584926364327
36	29.29097805870932	24.074675828329852	26.537906980161885	20.096439132798942
37	21.456155791818183	29.762559866232753	27.37305419435114	21.40823014759792
38	21.969703154928013	30.70727473722541	25.120140690749782	22.2028814170968
39	21.769999363168612	27.699471103367536	26.28056178325852	24.249967750205336
40	24.198857949043692	24.222045143180924	27.35941947103805	24.21967743673733
41	20.302347948341545	23.517285073488708	28.08801989526573	28.092347082904016
42	22.681403053851444	25.757135369125432	25.48329787216671	26.07816370485641
43	22.430507815880617	24.850630381429344	26.98948575048946	25.729376052200582
44	21.515185162808397	26.989404105439686	29.15724346717134	22.33816726458058
45	20.382196807025394	31.283362208465938	25.790609839534817	22.54383114497385
46	23.22899640271911	28.349610634757603	28.033970872312043	20.38742209021125
47	22.787949843813017	26.33844812355182	27.50205337300194	23.371548659633216
48	22.69528271231387	24.972934665998267	29.78664515591755	22.545137465770313
49	22.192757430924207	23.99825606173672	31.319775900667366	22.489210606671705
50	21.092345450003023	28.484161676793374	27.929220273445598	22.494272599758002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	600.0
1	1189.0
2	1778.0
3	8133.5
4	14489.0
5	10495.0
6	6501.0
7	6596.5
8	6692.0
9	6879.5
10	7067.0
11	7002.5
12	6938.0
13	6681.5
14	6425.0
15	6121.0
16	5817.0
17	5496.0
18	5175.0
19	5093.5
20	5012.0
21	5167.5
22	5323.0
23	5819.0
24	6315.0
25	7359.0
26	8403.0
27	9478.0
28	10553.0
29	12720.5
30	14888.0
31	17703.5
32	20519.0
33	23185.0
34	25851.0
35	28401.5
36	30952.0
37	36845.0
38	42738.0
39	54503.5
40	66269.0
41	80556.5
42	94844.0
43	99404.0
44	103964.0
45	105369.5
46	106775.0
47	106237.5
48	105700.0
49	101744.5
50	97789.0
51	88697.5
52	79606.0
53	73027.5
54	66449.0
55	62166.0
56	57883.0
57	55089.0
58	52295.0
59	48924.0
60	45553.0
61	40588.0
62	35623.0
63	30613.5
64	25604.0
65	21906.0
66	18208.0
67	16008.0
68	13808.0
69	11897.5
70	9987.0
71	8027.0
72	6067.0
73	4554.0
74	3041.0
75	2260.0
76	1479.0
77	1257.0
78	1035.0
79	760.5
80	486.0
81	325.5
82	165.0
83	117.0
84	69.0
85	55.5
86	42.0
87	27.5
88	13.0
89	12.0
90	11.0
91	11.0
92	11.0
93	6.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1224814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.821893756678488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7084323999737	19.950643876900298
2	7.652193152678121	4.257970097987867
3	3.6339832481310674	3.0331288752915593
4	2.287870463701542	2.5461155588058824
5	1.6842353866695532	2.342930899457931
6	1.3637460015627587	2.27651978199445
7	1.1085439413274896	2.1589254232156136
8	0.9469341125481069	2.107640021911045
9	0.7996851107499557	2.0023878771074646
>10	8.328793318240756	48.02120029346561
>50	0.4319256079310882	7.524708442316606
>100	0.04899140809585881	2.27469006667484
>500	0.003332748849998951	0.5415972884110237
>1k	0.0013330995399995803	0.9615414964599068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4834	0.39467217063162247	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2090	0.17063815403808252	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1889	0.15422749903250618	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1557	0.12712134250588253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013308143113974857	0.0
2	0.0	0.0	0.0	0.047762354120707305	0.0
3	8.164504977898685E-5	0.0	0.0	0.06286668832981987	0.0
4	8.164504977898685E-5	0.0	0.0	0.08556401216837822	0.0
5	8.164504977898685E-5	0.0	0.0	0.14630792920394445	0.0
6	8.164504977898685E-5	0.0	0.0	0.26902043902176165	0.0
7	8.164504977898685E-5	0.0	0.0	0.3459300759135673	0.0
8	8.164504977898685E-5	0.0	0.0	0.7902424368108137	0.0
9	8.164504977898685E-5	0.0	0.0	0.9909259691675634	0.0
10	8.164504977898685E-5	0.0	0.0	1.3235479019671559	0.0
11	8.164504977898685E-5	0.0	0.0	1.6120815078860953	0.0
12	8.164504977898685E-5	0.0	0.0	1.8473825413491354	0.0
13	8.164504977898685E-5	0.0	0.0	1.949275563473311	0.0
14	8.164504977898685E-5	0.0	0.0	1.9856892556747392	0.0
15	8.164504977898685E-5	0.0	0.0	2.0405547291262183	0.0
16	8.164504977898685E-5	0.0	0.0	2.1622874983466875	0.0
17	8.164504977898685E-5	0.0	0.0	2.296430315133563	0.0
18	8.164504977898685E-5	0.0	0.0	2.5190763658808604	0.0
19	8.164504977898685E-5	0.0	0.0	2.6094574359861986	0.0
20	8.164504977898685E-5	0.0	0.0	2.706451755123635	0.0
21	8.164504977898685E-5	0.0	0.0	2.845819855096366	0.0
22	8.164504977898685E-5	0.0	0.0	2.9786563510867774	0.0
23	8.164504977898685E-5	0.0	0.0	3.1338635907166315	0.0
24	8.164504977898685E-5	0.0	0.0	3.2573925510322383	0.0
25	8.164504977898685E-5	0.0	0.0	3.350304617680725	0.0
26	8.164504977898685E-5	0.0	0.0	3.4450945204741292	0.0
27	8.164504977898685E-5	0.0	0.0	3.5485387985441053	0.0
28	8.164504977898685E-5	0.0	0.0	3.657208359799937	0.0
29	8.164504977898685E-5	0.0	0.0	3.774532296332341	0.0
30	8.164504977898685E-5	0.0	0.0	3.9214933859345176	0.0
31	8.164504977898685E-5	0.0	0.0	4.048696373490179	0.0
32	8.164504977898685E-5	0.0	0.0	4.16226463773275	0.0
33	8.164504977898685E-5	0.0	0.0	4.284405632202114	0.0
34	8.164504977898685E-5	0.0	0.0	4.410955459359544	0.0
35	8.164504977898685E-5	0.0	0.0	4.57163291732459	0.0
36	8.164504977898685E-5	0.0	0.0	4.696631488536219	0.0
37	8.164504977898685E-5	0.0	0.0	4.826447117684808	0.0
38	8.164504977898685E-5	0.0	0.0	4.979286650871071	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTA	20	7.856685E-4	44.0	12
CTATACG	20	7.856685E-4	44.0	1
TCGCTAA	20	7.856685E-4	44.0	17
AATTCGC	70	0.0	44.0	13
CATACGT	20	7.856685E-4	44.0	30
ATCGGTA	20	7.856685E-4	44.0	44
CGCAATA	20	7.856685E-4	44.0	33
CGATACG	30	2.5278532E-6	44.0	12
CGTTTTA	2535	0.0	43.566074	1
TTACGGG	280	0.0	41.642857	3
CGCATGG	75	0.0	41.066666	2
CGTTATT	1495	0.0	41.056858	1
TAGGCCG	70	0.0	40.857143	6
GTTTTAT	2995	0.0	40.621037	2
ACACGAC	120	0.0	40.333332	26
TGCGTAA	60	3.6379788E-12	40.333332	1
CACGACC	115	0.0	40.173916	27
CGACTAG	55	7.8216544E-11	40.000004	2
GTACGGG	355	0.0	39.661972	3
TTACTGG	1850	0.0	39.48108	40
>>END_MODULE