FastQCFastQC Report
Thu 26 May 2016
SRR1545789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545789_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences468814
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.25937791960137707No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10990.2344213270081525No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5850.12478296296612303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG207.0290075E-445.0000042
CCTAGTG207.0290075E-445.00000413
GCGAATA351.2098644E-745.0000041
TAACGCC207.0290075E-445.00000412
CGTTCGA207.0290075E-445.00000414
GTCAACG207.0290075E-445.0000041
TTCGAAT207.0290075E-445.00000416
AGTACGG207.0290075E-445.0000042
TCAACGG207.0290075E-445.0000042
TTAGGCG453.8380676E-1045.05
TAACGGG502.1827873E-1145.03
CGTTTTA7300.044.0753441
GTAAGGC800.042.1875044
GTTTTAT8600.041.5988352
ACTAACC1250.041.39999814
ACGGTCT1250.041.39999830
CACGACG1200.041.24999626
AGGACTA2200.040.9090927
AGGCGAT3850.040.9090927
CGTTTTT11000.040.9090921