##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545789_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	468814
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.000853216840795	31.0	31.0	33.0	30.0	33.0
2	31.170602840358864	31.0	31.0	33.0	30.0	34.0
3	31.106323616615544	31.0	31.0	34.0	28.0	34.0
4	35.07784110542774	35.0	35.0	37.0	32.0	37.0
5	32.793988660748184	35.0	35.0	37.0	28.0	37.0
6	33.983326009888785	35.0	35.0	37.0	28.0	37.0
7	15.27317870200122	0.0	0.0	35.0	0.0	37.0
8	24.90712308079537	17.0	17.0	35.0	17.0	37.0
9	32.72229924874257	32.0	32.0	37.0	27.0	39.0
10	35.46284240658342	37.0	34.0	37.0	32.0	39.0
11	36.42924699347716	37.0	35.0	39.0	32.0	39.0
12	36.338539378090246	37.0	35.0	39.0	32.0	39.0
13	36.06847278451582	37.0	35.0	39.0	32.0	39.0
14	37.068415192379064	39.0	36.0	40.0	31.0	41.0
15	37.410450626474464	39.0	36.0	41.0	32.0	41.0
16	37.433929020891014	39.0	36.0	41.0	32.0	41.0
17	37.37296027848998	39.0	35.0	41.0	32.0	41.0
18	37.32111455715913	39.0	36.0	40.0	32.0	41.0
19	37.28327012418571	39.0	36.0	40.0	32.0	41.0
20	37.39233683294441	39.0	35.0	40.0	32.0	41.0
21	37.242593011300855	39.0	35.0	40.0	32.0	41.0
22	37.303301522565455	39.0	35.0	41.0	32.0	41.0
23	37.292450737392656	39.0	35.0	41.0	32.0	41.0
24	37.11667740297858	39.0	35.0	40.0	32.0	41.0
25	36.867169069183085	39.0	35.0	40.0	31.0	41.0
26	36.76012875042127	38.0	35.0	40.0	31.0	41.0
27	36.6598928359648	38.0	35.0	40.0	31.0	41.0
28	36.576032285725255	38.0	35.0	40.0	31.0	41.0
29	36.54998997470212	38.0	35.0	40.0	31.0	41.0
30	36.276751547522046	38.0	35.0	40.0	30.0	41.0
31	36.15791550593626	38.0	35.0	40.0	30.0	41.0
32	36.06676208475003	38.0	35.0	40.0	30.0	41.0
33	35.900265350437486	38.0	35.0	40.0	29.0	41.0
34	35.73294739491568	38.0	35.0	40.0	29.0	41.0
35	35.5386869846037	38.0	34.0	40.0	27.0	41.0
36	35.330246110397724	38.0	34.0	40.0	26.0	41.0
37	35.18528030306262	38.0	34.0	40.0	26.0	41.0
38	35.057547342869455	38.0	34.0	40.0	25.0	41.0
39	34.95643261506696	38.0	34.0	40.0	25.0	41.0
40	34.795479657177474	38.0	34.0	40.0	24.0	41.0
41	34.63655095624277	38.0	33.0	40.0	24.0	41.0
42	34.60102940611842	38.0	33.0	40.0	24.0	41.0
43	34.51497608859804	37.0	33.0	40.0	23.0	41.0
44	34.29555857973525	37.0	33.0	40.0	23.0	41.0
45	34.198801656947104	37.0	33.0	40.0	23.0	41.0
46	34.18243695794067	37.0	33.0	40.0	23.0	41.0
47	34.10539787634328	37.0	33.0	40.0	23.0	41.0
48	34.03193590635092	37.0	33.0	40.0	23.0	41.0
49	33.92403170553781	36.0	33.0	40.0	23.0	41.0
50	33.78954553404975	36.0	33.0	40.0	23.0	41.0
51	33.59741603279765	36.0	32.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	7.0
15	19.0
16	51.0
17	111.0
18	261.0
19	567.0
20	916.0
21	1585.0
22	2372.0
23	3458.0
24	4844.0
25	6617.0
26	7917.0
27	8900.0
28	9613.0
29	11208.0
30	14001.0
31	17691.0
32	23633.0
33	33945.0
34	43164.0
35	47294.0
36	57349.0
37	84603.0
38	73325.0
39	15359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.15064396541059	23.768274838208754	27.82724065407603	14.253840542304625
2	34.10286382232612	22.896500531127483	30.720285656998296	12.280349989548094
3	31.105726364826992	23.129642032874447	32.84436898215497	12.920262620143596
4	27.80121754043181	27.348586006390597	29.38777425588826	15.46242219728933
5	21.086187699172807	36.43107927664276	28.561647049789467	13.92108597439496
6	20.44968793594048	42.553976630390736	27.641452686993134	9.354882746675655
7	38.416088256750015	56.81805577478488	4.057259382185686	0.7085965862794199
8	88.29237181483488	2.847611206149987	6.9844330587397145	1.8755839202754185
9	84.05998967607623	4.443979915275568	8.584001331018271	2.9120290776299345
10	46.32604828354102	26.93136297124233	14.454346499891216	12.288242245325439
11	40.907694736078696	22.807126067054313	21.544151838468988	14.741027358398002
12	37.00230795155435	21.81398166437009	26.02162051474572	15.16208986932984
13	23.757396323488635	33.53611453582871	27.081955743642467	15.62453339704019
14	18.280170814011527	36.84638257389924	29.26576424765472	15.607682364434508
15	16.800266203654328	23.739692074042157	42.93963064242962	16.520411079873895
16	19.174128758953444	18.960184636124346	41.763897835815484	20.101788769106722
17	19.605429871974813	19.124002269556797	29.666349554407507	31.60421830406089
18	22.899486790070263	23.232454662190122	33.01458574189337	20.85347280584624
19	29.11623799630557	24.653487310532533	26.174772937668244	20.055501755493648
20	30.222220326184797	23.906922574837782	24.78509600822501	21.08576109075241
21	24.960432069008178	26.439056854104187	29.182575605677304	19.41793547121033
22	23.253571778999774	23.006778807800107	26.769678379911866	26.969971033288253
23	20.864564624776563	28.80353402415457	26.141710785087476	24.190190565981393
24	20.963111169888272	25.51758266604666	34.88505036112402	18.634255802941038
25	21.10837133703345	23.9617417568588	33.388721326581546	21.541165579526208
26	19.183940752622576	30.087412065339347	27.586420200761925	23.142226981276156
27	19.23342732938863	29.97905352655851	29.9090897456134	20.878429398439465
28	18.15880071840858	26.744508483108444	35.94495898160038	19.151731816882602
29	18.776956319563837	25.350565469461234	34.814233363338126	21.058244847636804
30	21.688345484563172	27.119497284637404	31.77422175958909	19.41793547121033
31	29.467123422082103	24.377684966745875	26.1099284577679	20.04526315340412
32	29.055659600609197	24.75224715985444	27.383141288442754	18.80895195109361
33	27.885046095039822	23.942970986361328	26.522032191871403	21.649950726727443
34	21.75041700973094	26.03527198419842	28.30973477754504	23.9045762285256
35	21.06123110657958	24.938888343778128	30.798141693720748	23.20173885592154
36	29.938099118200395	24.35720776256682	25.99709053057289	19.707602588659896
37	21.30205155989369	30.639016752912667	26.88635578289044	21.172575904303198
38	21.809715580166124	31.575422235684087	24.684843029431715	21.930019154718075
39	21.840857994855103	28.46054085415538	25.74261860780608	23.95598254318344
40	24.515052878113707	24.481350812902345	26.985968849053144	24.017627459930804
41	20.260700405704608	23.8478373086128	27.64785181329909	28.2436104723835
42	22.557773445332263	25.94589752012525	25.13043552453638	26.3658935100061
43	22.201342110090568	24.93611538904555	26.683716783201866	26.178825717662015
44	21.20393162320238	27.191807411894697	29.4389672663359	22.165293698567023
45	20.394228841288868	32.156676208475005	25.404744738851655	22.044350211384472
46	23.18958051594022	29.017691451193862	27.564876475531875	20.227851557334038
47	22.595101682117004	26.81852504404732	26.920911064942597	23.66546220889308
48	22.530470506426855	25.473002086115176	29.89927775194427	22.0972496555137
49	22.222885835320618	24.460233696092693	31.30644562662378	22.010434841962912
50	21.022623044533653	29.343406980166975	27.529681280849122	22.10428869445025
51	20.669177968234738	30.490130414194116	26.427325122543273	22.41336649502788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	451.0
2	644.0
3	2554.0
4	4464.0
5	3340.0
6	2216.0
7	2246.5
8	2277.0
9	2300.0
10	2323.0
11	2314.0
12	2305.0
13	2230.0
14	2155.0
15	2129.0
16	2103.0
17	2043.0
18	1983.0
19	1928.0
20	1873.0
21	1895.5
22	1918.0
23	2120.0
24	2322.0
25	2658.0
26	3499.0
27	4004.0
28	4626.0
29	5248.0
30	6144.5
31	7041.0
32	8307.5
33	9574.0
34	10441.5
35	11309.0
36	13162.5
37	15016.0
38	18808.5
39	22601.0
40	28076.0
41	33551.0
42	36060.0
43	38569.0
44	39298.5
45	40028.0
46	39777.5
47	39527.0
48	38268.5
49	37010.0
50	35140.5
51	33271.0
52	30502.0
53	27733.0
54	25526.0
55	23319.0
56	22002.5
57	20686.0
58	19437.0
59	18188.0
60	16503.5
61	14819.0
62	13067.5
63	11316.0
64	9826.5
65	8337.0
66	7138.0
67	5939.0
68	5163.0
69	4387.0
70	3770.0
71	3153.0
72	2640.0
73	2127.0
74	1607.5
75	797.5
76	507.0
77	424.0
78	341.0
79	258.0
80	175.0
81	119.0
82	63.0
83	41.0
84	19.0
85	18.5
86	18.0
87	14.0
88	10.0
89	6.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.27848478483761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.92071216014047	26.381804317821096
2	9.371148170111178	7.1742670529211905
3	4.934746025849315	5.6668380200253265
4	3.255214692364784	4.984187442922608
5	2.3642922287347714	4.525076205226687
6	1.8585957147112742	4.2686536672043935
7	1.4375823085795654	3.8519930768140225
8	1.189556330909596	3.6427531130744177
9	1.0010886574909013	3.448814124963507
>10	5.6099014005440155	33.322416605396334
>50	0.03773822445006977	0.9525612108937807
>100	0.017759164447090147	1.1645719353522193
>500	5.549738889715671E-4	0.12427482345513467
>1k	0.0011099477779431342	0.4917884039292936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1216	0.25937791960137707	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1099	0.2344213270081525	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	585	0.12478296296612303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012798252611910053	0.0
2	0.0	0.0	0.0	0.04991318518644921	0.0
3	0.0	0.0	0.0	0.062071525167763764	0.0
4	0.0	0.0	0.0	0.08553498828959887	0.0
5	0.0	0.0	0.0	0.14291382083299561	0.0
6	0.0	0.0	0.0	0.21757029440247092	0.0
7	0.0	0.0	0.0	0.26599035011753064	0.0
8	0.0	0.0	0.0	0.6475915821626487	0.0
9	0.0	0.0	0.0	0.8231409471560149	0.0
10	0.0	0.0	0.0	1.0972368572610887	0.0
11	0.0	0.0	0.0	1.335924268473211	0.0
12	0.0	0.0	0.0	1.547095436569727	0.0
13	0.0	0.0	0.0	1.6366832048530975	0.0
14	0.0	0.0	0.0	1.666332490070689	0.0
15	0.0	0.0	0.0	1.7126195036837637	0.0
16	0.0	0.0	0.0	1.8235376929869842	0.0
17	0.0	0.0	0.0	1.9380820538635792	0.0
18	0.0	0.0	0.0	2.1277094967300463	0.0
19	0.0	0.0	0.0	2.213884397650241	0.0
20	0.0	0.0	0.0	2.303472165933611	0.0
21	0.0	0.0	0.0	2.4075646205104797	0.0
22	0.0	0.0	0.0	2.520189243495288	0.0
23	0.0	0.0	0.0	2.6588369801243137	0.0
24	0.0	0.0	0.0	2.7661289978541594	0.0
25	0.0	0.0	0.0	2.8414253840542303	0.0
26	0.0	0.0	0.0	2.918428203935889	0.0
27	0.0	0.0	0.0	3.010149014321245	0.0
28	0.0	0.0	0.0	3.1042161710187837	0.0
29	0.0	0.0	0.0	3.2115081887486294	0.0
30	0.0	0.0	0.0	3.340770540128921	0.0
31	0.0	0.0	0.0	3.4508355125913477	0.0
32	0.0	0.0	0.0	3.562180310314965	0.0
33	0.0	0.0	0.0	3.6790710175037433	0.0
34	0.0	0.0	0.0	3.7848699057622	0.0
35	0.0	0.0	0.0	3.927570422384997	0.0
36	0.0	0.0	0.0	4.032942702223057	0.0
37	0.0	0.0	0.0	4.154952710456599	0.0
38	0.0	0.0	0.0	4.288694450251059	0.0
39	0.0	0.0	0.0	4.483654498372489	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.0290075E-4	45.000004	2
CCTAGTG	20	7.0290075E-4	45.000004	13
GCGAATA	35	1.2098644E-7	45.000004	1
TAACGCC	20	7.0290075E-4	45.000004	12
CGTTCGA	20	7.0290075E-4	45.000004	14
GTCAACG	20	7.0290075E-4	45.000004	1
TTCGAAT	20	7.0290075E-4	45.000004	16
AGTACGG	20	7.0290075E-4	45.000004	2
TCAACGG	20	7.0290075E-4	45.000004	2
TTAGGCG	45	3.8380676E-10	45.0	5
TAACGGG	50	2.1827873E-11	45.0	3
CGTTTTA	730	0.0	44.075344	1
GTAAGGC	80	0.0	42.187504	4
GTTTTAT	860	0.0	41.598835	2
ACTAACC	125	0.0	41.399998	14
ACGGTCT	125	0.0	41.399998	30
CACGACG	120	0.0	41.249996	26
AGGACTA	220	0.0	40.909092	7
AGGCGAT	385	0.0	40.909092	7
CGTTTTT	1100	0.0	40.909092	1
>>END_MODULE