FastQCFastQC Report
Thu 26 May 2016
SRR1545788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545788_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1129850
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42740.378280302695048No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.15497632429083505No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17130.1516130459795548No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13070.11567907244324467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC302.5276786E-644.00000427
TAGTACG302.5276786E-644.0000041
TATTTCG254.442793E-544.040
GTCGAAA207.8564254E-444.01
TATGCGA254.442793E-544.01
TGATTCG502.7284841E-1144.015
TACGACG207.8564254E-444.01
GTGTCGA207.8564254E-444.01
GCGATAC900.044.09
AATCGGC207.8564254E-444.039
CGTTTTA24650.043.0182531
AGGCGAT9700.042.412377
CGTTATT11700.042.307691
CACGACG3100.041.16129326
CGCATCG650.040.61538321
TCACGAC3150.040.50793525
CGGTCTA3050.040.39344431
CATATGC6150.040.06503733
CGATACG557.8216544E-1140.010
CGACGGT3200.039.87528
Produced by FastQC (version 0.11.5)