##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545788_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1129850
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51047041642696	31.0	31.0	33.0	30.0	34.0
2	31.696708412621145	31.0	31.0	34.0	30.0	34.0
3	31.755262202947293	31.0	31.0	34.0	30.0	34.0
4	35.663617294331104	37.0	35.0	37.0	33.0	37.0
5	27.106245961853343	35.0	25.0	37.0	0.0	37.0
6	31.335764924547508	35.0	26.0	37.0	17.0	37.0
7	15.034837367792186	0.0	0.0	32.0	0.0	37.0
8	25.125125459131745	17.0	17.0	35.0	17.0	37.0
9	33.24440943488074	32.0	32.0	37.0	28.0	39.0
10	36.03498606009647	37.0	35.0	39.0	32.0	39.0
11	36.987493915121476	39.0	37.0	39.0	34.0	39.0
12	37.00182767624021	39.0	35.0	39.0	33.0	39.0
13	36.52001239102536	39.0	35.0	39.0	33.0	39.0
14	37.73734389520733	40.0	36.0	41.0	33.0	41.0
15	38.029919015798555	40.0	36.0	41.0	33.0	41.0
16	38.114841793158384	40.0	36.0	41.0	33.0	41.0
17	38.14685135194937	40.0	36.0	41.0	34.0	41.0
18	38.13864760808957	40.0	36.0	41.0	34.0	41.0
19	38.08436960658494	40.0	36.0	41.0	34.0	41.0
20	38.119281320529275	40.0	36.0	41.0	34.0	41.0
21	38.00228348895871	40.0	36.0	41.0	34.0	41.0
22	37.9449378236049	40.0	36.0	41.0	34.0	41.0
23	37.853990352701686	40.0	35.0	41.0	34.0	41.0
24	37.723388060362	40.0	35.0	41.0	33.0	41.0
25	37.531577643049964	39.0	35.0	41.0	33.0	41.0
26	37.29539938929946	39.0	35.0	40.0	33.0	41.0
27	37.09238482984467	39.0	35.0	40.0	32.0	41.0
28	37.12528300216843	39.0	35.0	40.0	32.0	41.0
29	37.0312917643935	39.0	35.0	40.0	32.0	41.0
30	36.82531752002478	38.0	35.0	40.0	32.0	41.0
31	36.635439217595255	38.0	35.0	40.0	31.0	41.0
32	36.454356773022965	38.0	35.0	40.0	31.0	41.0
33	36.26604505022791	38.0	35.0	40.0	30.0	41.0
34	36.01388325883967	38.0	35.0	40.0	30.0	41.0
35	35.8275611806877	38.0	35.0	40.0	30.0	41.0
36	35.63400982431296	38.0	35.0	40.0	29.0	41.0
37	35.52568748063902	38.0	35.0	40.0	29.0	41.0
38	35.29199451254591	38.0	34.0	40.0	28.0	41.0
39	35.23214497499668	38.0	34.0	40.0	27.0	41.0
40	34.97413461963978	38.0	34.0	40.0	26.0	41.0
41	34.76193477010223	37.0	34.0	40.0	26.0	40.0
42	34.769504801522324	37.0	34.0	40.0	26.0	40.0
43	34.64105146700889	37.0	34.0	40.0	26.0	40.0
44	34.443347346992965	37.0	33.0	40.0	25.0	40.0
45	34.24379873434527	36.0	33.0	39.0	24.0	40.0
46	34.21019073328318	36.0	33.0	39.0	24.0	40.0
47	34.010924458998986	36.0	33.0	39.0	24.0	40.0
48	33.907417798822856	36.0	33.0	39.0	24.0	40.0
49	33.786675222374654	36.0	33.0	39.0	24.0	40.0
50	33.85571536044608	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	2.0
13	4.0
14	11.0
15	22.0
16	53.0
17	172.0
18	361.0
19	706.0
20	1398.0
21	2422.0
22	4050.0
23	6215.0
24	9289.0
25	13338.0
26	17021.0
27	19036.0
28	20169.0
29	23069.0
30	28732.0
31	37838.0
32	52112.0
33	90704.0
34	94425.0
35	118720.0
36	158847.0
37	278479.0
38	121408.0
39	31245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.92693720405364	23.308403770412	27.492056467672697	14.272602557861664
2	34.405009514537326	23.83059698190025	29.45629950878435	12.308093994778067
3	31.08855157764305	23.558613975306457	32.57184582024163	12.780988626808867
4	27.273885914059388	26.627605434349693	30.982962340133646	15.115546311457273
5	17.33575253352215	47.74775412665398	23.555250696995174	11.361242642828694
6	20.294463866884985	41.273443377439484	28.359693764659028	10.072398991016506
7	39.690666902686196	55.54259414966588	3.7549232198964466	1.0118157277514714
8	88.00858521042616	2.5956542903925297	7.665707837323539	1.7300526618577685
9	84.30906757534186	3.8111253706244193	9.000752312253839	2.8790547417798824
10	48.78213922202062	23.872637960791256	14.766650440323936	12.578572376864186
11	41.26937204053635	21.843519051201486	21.492587511616588	15.394521396645573
12	37.4649732265345	20.235340974465636	26.971190866044164	15.328494932955703
13	24.38828163030491	30.94233747842634	28.270301367438154	16.399079523830597
14	18.938620170819135	34.211178475018805	30.33066336239324	16.519537991768818
15	17.369650838606894	22.535911846705314	42.82099393724831	17.27344337743948
16	20.713280523963356	17.738549364959947	40.69398592733548	20.854184183741204
17	20.555117936009204	18.023188918883037	29.51551090852768	31.906182236580076
18	23.52799044120901	21.94317829800416	33.76103022525114	20.76780103553569
19	28.78346683188034	24.090631499756604	25.998229853520378	21.127671814842678
20	29.41284241270965	24.01584281099261	25.29919900871797	21.272115767579766
21	25.388768420586803	25.34026640704518	29.69774748860468	19.573217683763332
22	24.104969686241535	21.509049873877064	28.088949860600966	26.297030579280435
23	21.6490684604151	27.765544098774175	26.38713103509315	24.198256405717572
24	22.401380714254103	24.897818294463868	33.444882063990796	19.255918927291233
25	22.66654865690136	22.85798999867239	32.05159976988096	22.423861574545292
26	19.890870469531354	28.672744169580035	27.131477629773865	24.30490773111475
27	20.379607912554764	27.301942735761386	30.337743948311726	21.980705403372127
28	18.758950303137585	25.943886356596007	34.92437049165819	20.372792848608224
29	20.438111253706246	24.92667168208169	33.17697039430013	21.458246669911933
30	22.41598442271098	25.887772713192014	31.328583440279683	20.367659423817322
31	29.31486480506262	23.720139841571893	26.375536575651637	20.58945877771386
32	29.360180554940925	24.711510377483737	26.10461565694561	19.82369341062973
33	28.154445280346952	24.071425410452715	26.161437358941452	21.612691950258885
34	21.100677081028454	25.671283798734347	29.021108996769485	24.206930123467718
35	21.916803115457803	24.31004115590565	29.87219542417135	23.900960304465194
36	30.31048369252556	23.510997035004646	25.399035270168607	20.779484002301192
37	22.48634774527592	29.115723326105236	26.66336239323804	21.734566535380804
38	22.856662388812673	29.653316811966192	24.338540514227553	23.151480284993582
39	22.04584679382219	27.23202194981635	25.217683763331415	25.504447493030046
40	24.720538124529806	23.157410275700315	26.910386334469177	25.211665265300702
41	20.229942027702794	22.294906403504893	27.982741071823693	29.492410496968624
42	22.4528034694871	25.196707527547908	25.62065760941718	26.729831393547816
43	21.99168031154578	24.41837412045847	27.259193698278533	26.33075186971722
44	21.852546798247555	25.784042129486217	29.08695844581139	23.276452626454837
45	20.85710492543258	29.18192680444307	25.37611187325751	24.58485639686684
46	23.720139841571893	26.982077266893832	27.871133336283577	21.426649555250695
47	22.60238084701509	26.634243483648273	27.447094747090322	23.316280922246317
48	22.445103332300747	25.80448732132584	28.72354737354516	23.026861972828254
49	22.4794441740054	24.00070805859185	30.504668761340003	23.015179006062752
50	21.340620436341105	27.415674647077044	28.15957870513785	23.084126211443998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	1028.5
2	1659.0
3	7388.0
4	13117.0
5	9397.0
6	5677.0
7	5745.0
8	5813.0
9	5885.0
10	5957.0
11	5894.5
12	5832.0
13	5544.5
14	5257.0
15	5015.0
16	4773.0
17	4564.5
18	4356.0
19	4240.5
20	4125.0
21	4471.0
22	4817.0
23	5203.5
24	5590.0
25	6276.5
26	6963.0
27	8002.0
28	9041.0
29	10536.0
30	12031.0
31	14307.0
32	16583.0
33	19008.5
34	21434.0
35	23842.5
36	26251.0
37	30373.0
38	34495.0
39	45175.0
40	55855.0
41	67391.5
42	78928.0
43	83521.0
44	88114.0
45	89665.5
46	91217.0
47	94305.0
48	97393.0
49	94410.0
50	91427.0
51	82690.0
52	73953.0
53	68532.0
54	63111.0
55	62649.0
56	62187.0
57	60411.5
58	58636.0
59	53720.5
60	48805.0
61	43937.5
62	39070.0
63	33912.5
64	28755.0
65	24329.5
66	19904.0
67	17001.5
68	14099.0
69	12000.0
70	9901.0
71	8450.5
72	7000.0
73	5439.5
74	3879.0
75	2831.0
76	1783.0
77	1316.5
78	850.0
79	644.0
80	438.0
81	337.5
82	237.0
83	156.5
84	76.0
85	53.5
86	31.0
87	22.5
88	14.0
89	11.0
90	8.0
91	7.5
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1129850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.323471004734184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11065023019319	19.85776968917937
2	7.863685491263123	4.4545373600428
3	3.8345265672113813	3.258213061298834
4	2.6042455062356478	2.950450883402987
5	1.9205803535130885	2.7198750977495045
6	1.5312437326039021	2.6022082476952546
7	1.2462041954086813	2.470777987624512
8	1.1113671630884026	2.5182220495478447
9	0.9527136259223816	2.428574108366498
>10	8.470709181234941	47.44903804309741
>50	0.3045385672393219	5.446766542711844
>100	0.04430277487972071	2.2894818355684463
>500	0.0038372482179097884	0.660404651687239
>1k	0.0013953629883308321	0.8936804420273904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4274	0.378280302695048	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.15497632429083505	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1713	0.1516130459795548	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1307	0.11567907244324467	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013630127893083153	0.0
2	0.0	0.0	0.0	0.0436341107226623	0.0
3	0.0	0.0	0.0	0.06080453157498783	0.0
4	0.0	0.0	0.0	0.08284285524627163	0.0
5	0.0	0.0	0.0	0.13904500597424438	0.0
6	0.0	0.0	0.0	0.2520688586980573	0.0
7	0.0	0.0	0.0	0.3279196353498252	0.0
8	0.0	0.0	0.0	0.7106253042439262	0.0
9	0.0	0.0	0.0	0.8784351905120149	0.0
10	0.0	0.0	0.0	1.1926361906447758	0.0
11	0.0	0.0	0.0	1.4779838031597115	0.0
12	0.0	0.0	0.0	1.7501438244014693	0.0
13	0.0	0.0	0.0	1.858476788954286	0.0
14	0.0	0.0	0.0	1.9044120901004558	0.0
15	0.0	0.0	0.0	1.9646855777315573	0.0
16	0.0	0.0	0.0	2.0857635969376465	0.0
17	0.0	0.0	0.0	2.2327742620701865	0.0
18	0.0	0.0	0.0	2.457051821038191	0.0
19	0.0	0.0	0.0	2.5519316723458867	0.0
20	0.0	0.0	0.0	2.6560162853476124	0.0
21	0.0	0.0	0.0	2.787538168783467	0.0
22	0.0	0.0	0.0	2.92649466743373	0.0
23	0.0	0.0	0.0	3.0922688852502547	0.0
24	0.0	0.0	0.0	3.2143204850201355	0.0
25	0.0	0.0	0.0	3.3128291366110547	0.0
26	0.0	0.0	0.0	3.414435544541311	0.0
27	0.0	0.0	0.0	3.5386998274107184	0.0
28	0.0	0.0	0.0	3.650307562950834	0.0
29	0.0	0.0	0.0	3.7806788511749345	0.0
30	0.0	0.0	0.0	3.9484887374430233	0.0
31	0.0	0.0	0.0	4.090277470460681	0.0
32	0.0	0.0	0.0	4.216488914457671	0.0
33	0.0	0.0	0.0	4.352170642120636	0.0
34	0.0	0.0	0.0	4.504314732044077	0.0
35	0.0	0.0	0.0	4.696464132406956	0.0
36	0.0	0.0	0.0	4.841439129087932	0.0
37	0.0	0.0	0.0	4.998539629154313	0.0
38	0.0	0.0	0.0	5.204407664734257	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	30	2.5276786E-6	44.000004	27
TAGTACG	30	2.5276786E-6	44.000004	1
TATTTCG	25	4.442793E-5	44.0	40
GTCGAAA	20	7.8564254E-4	44.0	1
TATGCGA	25	4.442793E-5	44.0	1
TGATTCG	50	2.7284841E-11	44.0	15
TACGACG	20	7.8564254E-4	44.0	1
GTGTCGA	20	7.8564254E-4	44.0	1
GCGATAC	90	0.0	44.0	9
AATCGGC	20	7.8564254E-4	44.0	39
CGTTTTA	2465	0.0	43.018253	1
AGGCGAT	970	0.0	42.41237	7
CGTTATT	1170	0.0	42.30769	1
CACGACG	310	0.0	41.161293	26
CGCATCG	65	0.0	40.615383	21
TCACGAC	315	0.0	40.507935	25
CGGTCTA	305	0.0	40.393444	31
CATATGC	615	0.0	40.065037	33
CGATACG	55	7.8216544E-11	40.0	10
CGACGGT	320	0.0	39.875	28
>>END_MODULE